Reviewed, UniProtKB/Swiss-Prot A2QGD9 (ATG15_ASPNC)

Names and origin

Protein names

Recommended name:
    Putative lipase atg15
    EC=3.1.1.3
Alternative name(s):
    Autophagy-related protein 15

Gene names

Name:	atg15
ORF Names:	An03g02820

Organism

Aspergillus niger (strain CBS 513.88 / FGSC A1513) [Complete proteome]

Taxonomic identifier

425011 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	595 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Autophagy Lipid degradation
Cellular component	Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endosome Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 595	595	Putative lipase atg15		PRO_5000219762
Regions
	Topological domain	1 – 20	20	Cytoplasmic By similarity
	Transmembrane	21 – 41	21	Signal-anchor for type II membrane protein
	Topological domain	42 – 595	554	Lumenal By similarity
	Compositional bias	470 – 588	119	Thr-rich
Sites
	Active site	319	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	164	1	N-linked (GlcNAc...) Potential
	Glycosylation	199	1	N-linked (GlcNAc...) Potential
	Glycosylation	221	1	N-linked (GlcNAc...) Potential
	Glycosylation	279	1	N-linked (GlcNAc...) Potential
	Glycosylation	303	1	N-linked (GlcNAc...) Potential
	Glycosylation	465	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A2QGD9-1 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: EC1983AFC1D703BA	FASTA	595	64,654
	10 20 30 40 50 60 MKGLRGHNKK SFWGNTRLSD LLWPVTLLPG LISAYQPVYL GSRQSSPFLP PQIPLGDSPP 70 80 90 100 110 120 LSDTHEFTLR HIFHRGTYQD PDLHRRLDIT PDTRLRAVSD AGIESDVVTP NTPLVASSQP 130 140 150 160 170 180 LIIERLADRR LSVIEEHLVT ARSSGSAVAL SPSDWVMDTL PGPNVTEKKT VVALAMMTAN 190 200 210 220 230 240 DYIEVPGTGD WQDIHGRFNH SGSFGWQGDG LRGHVYADKT NSTVVISLKG TSPALFDGEG 250 260 270 280 290 300 TTTNDKINDN LFFSCCCGQG GSYLWRQVCD CQTTLYNANL TCIVEAMLDE NRYYRASLDL 310 320 330 340 350 360 YSNITEMYPN ANVWLTGHSL GGAVSSLLGL TFGVPVVTFE AVPEALPAAR LGLPSPPGHD 370 380 390 400 410 420 SRYPQSRRNT GSYHFGHTAD PVYMGTCNGV SSVCTWGGYA MESACHTGQM CVYDTVEDKG 430 440 450 460 470 480 WRVGLSKHRI NYVIANVLAG YDDVPPCAPE EECYDCELWK FFRSNGSEIT TTTSATSTST 490 500 510 520 530 540 STTTSRTSTC KTPGWWGCLD QTTTTETTST STSTSTCKTP GWFGCKDPTT TSDITSVPTI 550 560 570 580 590 TTTEPPMTST TTCKTPGWFG CKDETTTQVI APAATLTITE PPVTSTTTGK HLGRF

References

[1]

"Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88." Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G., Schaap P.J., Turner G., de Vries R.P., Albang R., Albermann K., Andersen M.R., Bendtsen J.D., Benen J.A.E., van den Berg M., Breestraat S., Caddick M.X., Contreras R., Cornell M. , Coutinho P.M., Danchin E.G.J., Debets A.J.M., Dekker P., van Dijck P.W.M., van Dijk A., Dijkhuizen L., Driessen A.J.M., d'Enfert C., Geysens S., Goosen C., Groot G.S.P., de Groot P.W.J., Guillemette T., Henrissat B., Herweijer M., van den Hombergh J.P.T.W., van den Hondel C.A.M.J.J., van der Heijden R.T.J.M., van der Kaaij R.M., Klis F.M., Kools H.J., Kubicek C.P., van Kuyk P.A., Lauber J., Lu X., van der Maarel M.J.E.C., Meulenberg R., Menke H., Mortimer M.A., Nielsen J., Oliver S.G., Olsthoorn M., Pal K., van Peij N.N.M.E., Ram A.F.J., Rinas U., Roubos J.A., Sagt C.M.J., Schmoll M., Sun J., Ussery D., Varga J., Vervecken W., van de Vondervoort P.J.J., Wedler H., Woesten H.A.B., Zeng A.-P., van Ooyen A.J.J., Visser J., Stam H. Nat. Biotechnol. 25:221-231(2007) [PubMed: 17259976] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM270049 Genomic DNA. Translation: CAK44586.1.
RefSeq	XP_001390178.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4980272.
GenomeReviews	Gene locus atg15 in contig AM270982_GR.
KEGG	ang:An03g02820.
Enzyme and pathway databases
BRENDA	3.1.1.3. 277.
Family and domain databases
InterPro	IPR002921. Lipase_3. IPR008262. Lipase_Ser_AS. [Graphical view]
Pfam	PF01764. Lipase_3. 1 hit. [Graphical view]
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG15_ASPNC

Accession

Primary (citable) accession number: A2QGD9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	March 6, 2007
Last modified:	October 13, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)