A2QD49 (MTLD_ASPNC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannitol-1-phosphate 5-dehydrogenase
Short name=M1PDH
Short name=MPD
Short name=MPDH
EC=1.1.1.17

Gene names

Name:	mpdA
ORF Names:	An02g05830

Organism

Aspergillus niger (strain CBS 513.88 / FGSC A1513) [Complete proteome]

Taxonomic identifier

425011 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NAD(H)-dependent interconversion of D-fructose 6-phosphate and D-mannitol 1-phosphate in the metabolism of mannitol. Has a strong preference for NADH over NADPH By similarity.
Catalytic activity	D-mannitol 1-phosphate + NAD+ = D-fructose 6-phosphate + NADH.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the mannitol dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro mannitol-1-phosphate 5-dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Mannitol-1-phosphate 5-dehydrogenase		PRO_0000371521
Regions
	Nucleotide binding	5 – 16	12	NAD By similarity
Sites
	Active site	213	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2QD49 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 310A8C21416DD6D0	FASTA	388	43,231
	10 20 30 40 50 60 MGKKAIQFGG GNIGRGFVAE FLHKAGYEVV FVDVMDKMVE ALQQNKSYKV TEVSEEGEHT 70 80 90 100 110 120 TTITNYRAIN SKTHESDVIQ EIATADVVTC AVGPNILKFI APVIAKGIDA RTESKPVAVI 130 140 150 160 170 180 ACENAIGATD TLHGFIKQHT SQDRVESLYD RAQFANSAID RIVPQQAPNS GLDVRIEKFY 190 200 210 220 230 240 EWAVEKTPFG SVGHPDIPAI HWVDNLEPYI ERKLFTVNTS HATTAYFGHF RGKKMIADAL 250 260 270 280 290 300 EDKEIRGLVH KVLEETASLI VAKHDISEEE QKEYVEKIVS RISNPYLEDN VDRVGRAPLR 310 320 330 340 350 360 KLSRKERFIG PASQLAERGM KYDSLMDAVE MALRFQNVPG DDESAELANI LQEQPAEDAT 370 380 TNLTGLEKEH PLYPAVLDRV RKVQQGTK

References

[1]

"Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88." Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G., Schaap P.J., Turner G., de Vries R.P., Albang R., Albermann K., Andersen M.R., Bendtsen J.D., Benen J.A.E., van den Berg M., Breestraat S., Caddick M.X., Contreras R., Cornell M. , Coutinho P.M., Danchin E.G.J., Debets A.J.M., Dekker P., van Dijck P.W.M., van Dijk A., Dijkhuizen L., Driessen A.J.M., d'Enfert C., Geysens S., Goosen C., Groot G.S.P., de Groot P.W.J., Guillemette T., Henrissat B., Herweijer M., van den Hombergh J.P.T.W., van den Hondel C.A.M.J.J., van der Heijden R.T.J.M., van der Kaaij R.M., Klis F.M., Kools H.J., Kubicek C.P., van Kuyk P.A., Lauber J., Lu X., van der Maarel M.J.E.C., Meulenberg R., Menke H., Mortimer M.A., Nielsen J., Oliver S.G., Olsthoorn M., Pal K., van Peij N.N.M.E., Ram A.F.J., Rinas U., Roubos J.A., Sagt C.M.J., Schmoll M., Sun J., Ussery D., Varga J., Vervecken W., van de Vondervoort P.J.J., Wedler H., Woesten H.A.B., Zeng A.-P., van Ooyen A.J.J., Visser J., Stam H. Nat. Biotechnol. 25:221-231(2007) [PubMed: 17259976] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CBS 513.88 / FGSC A1513.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM270011 Genomic DNA. Translation: CAK47711.1.
RefSeq	XP_001399719.1. XM_001399682.2.
3D structure databases
ProteinModelPortal	A2QD49.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADANGAT00002099; CADANGAP00002039; CADANGAG00002099.
GeneID	4979072.
GenomeReviews	Gene locus mpdA in contig AM270981_GR.
KEGG	ang:ANI_1_802024.
Phylogenomic databases
GeneTree	EFGT00050000004394.
HOGENOM	HBG568825.
OrthoDB	EOG469V3C.
Family and domain databases
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013328. DH_multihelical. IPR023028. Mannitol_1_phos_5_DH. IPR000669. Mannitol_DH. IPR013118. Mannitol_DH_C. IPR013131. Mannitol_DH_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00009.
Pfam	PF01232. Mannitol_dh. 1 hit. PF08125. Mannitol_dh_C. 1 hit. [Graphical view]
PRINTS	PR00084. MTLDHDRGNASE.
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
PROSITE	PS00974. MANNITOL_DHGENASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTLD_ASPNC

Accession

Primary (citable) accession number: A2QD49

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	March 6, 2007
Last modified:	December 14, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program