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Protein

Probable endo-1,3(4)-beta-glucanase An02g00850

Gene

An02g00850

Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Mixed-linked glucanase involved in the degradation of complex natural cellulosic substrates.By similarity

Catalytic activityi

Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei140NucleophileBy similarity1
Active sitei145Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable endo-1,3(4)-beta-glucanase An02g00850 (EC:3.2.1.6)
Alternative name(s):
Mixed-linked glucanase An02g00850
Gene namesi
ORF Names:An02g00850
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006706 Componenti: Chromosome 4R

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_500021953025 – 717Probable endo-1,3(4)-beta-glucanase An02g00850Add BLAST693
PropeptideiPRO_0000395090718 – 739Removed in mature formSequence analysisAdd BLAST22

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi59N-linked (GlcNAc...)Sequence analysis1
Glycosylationi74N-linked (GlcNAc...)Sequence analysis1
Glycosylationi396N-linked (GlcNAc...)Sequence analysis1
Glycosylationi459N-linked (GlcNAc...)Sequence analysis1
Glycosylationi482N-linked (GlcNAc...)Sequence analysis1
Glycosylationi526N-linked (GlcNAc...)Sequence analysis1
Glycosylationi537N-linked (GlcNAc...)Sequence analysis1
Glycosylationi569N-linked (GlcNAc...)Sequence analysis1
Glycosylationi581N-linked (GlcNAc...)Sequence analysis1
Glycosylationi592N-linked (GlcNAc...)Sequence analysis1
Glycosylationi620N-linked (GlcNAc...)Sequence analysis1
Lipidationi717GPI-anchor amidated glycineSequence analysis1

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Structurei

3D structure databases

ProteinModelPortaliA2QBQ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 283GH16PROSITE-ProRule annotationAdd BLAST253

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi287 – 292Poly-Ser6
Compositional biasi325 – 686Ala-richAdd BLAST362
Compositional biasi419 – 706Ser-richAdd BLAST288

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.Curated
Contains 1 GH16 (glycosyl hydrolase family 16) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000166269.
OrthoDBiEOG092C3D34.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. GH16.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
PROSITEiPS51762. GH16_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2QBQ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTSTLLWSV GSLALSSMVL PAAASSYELV ETWKGEDFLT AFDFYTGADP
60 70 80 90 100
TNGFVTYANQ SYAESTGLVK VNSNGTFYMG VDHTTKLSTN GPGRESVRIG
110 120 130 140 150
SNKYYDEGLF IIDLQHMPGS VCGTWPAFWS TGKNWPTDGE IDIIEGVNKN
160 170 180 190 200
EANEIVLHTS GTCQVSSQKM SGTLTSTECG EDAGTTGCVV EGTQGSSGTP
210 220 230 240 250
FNENGGGVYA MQWTDEFLKF WFFPRGSIPS SITKGDPDVA AFGTPMAHME
260 270 280 290 300
GSCSIAEHFK AQQFIFDTTF CGDWAGGVYS TSGCPVSDSS SSFKSCVAYV
310 320 330 340 350
AENPTAFAES YWEINYIKIY QTGVAASASA SASHVESAAS VAETISAVRE
360 370 380 390 400
GTNSVVATTT AATIAVTSTA DAETATTMAG ATTVAASTET SAADENGSSA
410 420 430 440 450
STSTHYVTMT TTICPIAESS SAAAALAGGS SEATEASNSE GSPAAATPSS
460 470 480 490 500
VTGASAEANE SESTSAAVTT PSTSTGASAE ANGSESSSAS EAGSVAGATP
510 520 530 540 550
SSVSTTGASG EADSSEGASA AATPSNVSST GASAEANGSE DSSASSEAKT
560 570 580 590 600
VAPSIPSSVT GASAEANGND SASSNAATAS NVSGASAQAG DNESTPASAG
610 620 630 640 650
ANAGSSAAPS SVSGASAEAN GSEGSSSHSS GSQAGAHSYG SVPSSAAAYG
660 670 680 690 700
RPAPSSSSHA FATAPSSTGS SRVPTSAAAA NNAAAATQGS SASGSNSGSS
710 720 730
GHGSSSATTP STPVFTGGAN KLTLGASSVL SVLAFALLA
Length:739
Mass (Da):72,556
Last modified:March 6, 2007 - v1
Checksum:i7640750996F31B92
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM269996 Genomic DNA. Translation: CAK96300.1.
RefSeqiXP_001399223.1. XM_001399186.1.

Genome annotation databases

EnsemblFungiiCAK96300; CAK96300; An02g00850.
GeneIDi4978566.
KEGGiang:ANI_1_2196024.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM269996 Genomic DNA. Translation: CAK96300.1.
RefSeqiXP_001399223.1. XM_001399186.1.

3D structure databases

ProteinModelPortaliA2QBQ3.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAK96300; CAK96300; An02g00850.
GeneIDi4978566.
KEGGiang:ANI_1_2196024.

Phylogenomic databases

HOGENOMiHOG000166269.
OrthoDBiEOG092C3D34.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. GH16.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
PROSITEiPS51762. GH16_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEGLX_ASPNC
AccessioniPrimary (citable) accession number: A2QBQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: March 6, 2007
Last modified: November 30, 2016
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.