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A2QAS2

- MNS1B_ASPNC

UniProt

A2QAS2 - MNS1B_ASPNC

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Protein
Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B
Gene
mns1B, msdS, An01g12550
Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Involved in the maturation of Asn-linked oligosaccharides. Progressively trims alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2 By similarity.

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

Cofactori

Ca2+. Can also use Mg2+, but with lower efficiency By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei377 – 3771Proton donor By similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular protein catabolic process Source: ASPGD
  2. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B (EC:3.2.1.113)
Alternative name(s):
Class I alpha-mannosidase 1B
Man(9)-alpha-mannosidase 1B
Gene namesi
Name:mns1B
Synonyms:msdS
ORF Names:An01g12550
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006706: Chromosome 2R

Subcellular locationi

GO - Cellular componenti

  1. cytoplasmic membrane-bounded vesicle lumen Source: UniProtKB-SubCell
  2. membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed prediction
Add
BLAST
Chaini22 – 513492Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B
PRO_5000219468Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi97 – 971N-linked (GlcNAc...) Reviewed prediction
Glycosylationi117 – 1171N-linked (GlcNAc...) Reviewed prediction
Glycosylationi184 – 1841N-linked (GlcNAc...) Reviewed prediction
Glycosylationi251 – 2511N-linked (GlcNAc...) Reviewed prediction
Glycosylationi322 – 3221N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi334 ↔ 363 By similarity
Glycosylationi348 – 3481N-linked (GlcNAc...) Reviewed prediction
Glycosylationi368 – 3681N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Monomer By similarity.

Protein-protein interaction databases

STRINGi5061.CADANGAP00001212.

Structurei

3D structure databases

ProteinModelPortaliA2QAS2.
SMRiA2QAS2. Positions 38-511.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG300315.
HOGENOMiHOG000181987.
KOiK01230.
OrthoDBiEOG7BP8BH.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERiPTHR11742. PTHR11742. 1 hit.
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2QAS2-1 [UniParc]FASTAAdd to Basket

« Hide

MHLSSLSLSL TALAIVSPSA AYPHLGSSQP VLHTNSDTTQ SRADAIKAAF    50
SHAWDGYLQY AFPHDELHPV SNGYGDSRNG WGASAVDALS TAVIMRNATI 100
VNQILDHVAK IDYSKTNTTV SLFETTIRYL GGMLSGYDLL KGPVSDLVQD 150
SSKIDVLLTQ SKNLGDVLKF AFDTPSGVPY NNLNITSGGN DGAKTNGLAV 200
TGTLALEWTR LSDLTGDTTY ADLSQKAESY LLNPQPKSAE PFPGLVGSNI 250
NISNGQFTDA QVSWNGGDDS YYEYLIKMYV YDPKRFGLYK DRWVAAAQST 300
MQHLASHPST RPDLTFLASY NNGTLGLSSQ HLTCFDGGSF LLGGTVLNRT 350
DFINFGLDLV SGCHDTYNST LTGIGPESFS WDTSDIPSSQ QSLYEKAGFY 400
ITSGAYILRP EVIESFYYAW RATGQETYRE WIWSAFSAVN DYCRTDSGFS 450
GLTDVNAANG GSRYDNQESF LFAEVMKYSY MAFAEDAAWQ VQPGSGNQFV 500
FNTEAHPVRV SST 513
Length:513
Mass (Da):55,929
Last modified:March 6, 2007 - v1
Checksum:i92F9FBA75F09C927
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM269984 Genomic DNA. Translation: CAK37306.1.
RefSeqiXP_001389661.1. XM_001389624.2.

Genome annotation databases

EnsemblFungiiCADANGAT00001254; CADANGAP00001212; CADANGAG00001254.
GeneIDi4977321.
KEGGiang:ANI_1_1702014.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM269984 Genomic DNA. Translation: CAK37306.1 .
RefSeqi XP_001389661.1. XM_001389624.2.

3D structure databases

ProteinModelPortali A2QAS2.
SMRi A2QAS2. Positions 38-511.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5061.CADANGAP00001212.

Protein family/group databases

CAZyi GH47. Glycoside Hydrolase Family 47.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CADANGAT00001254 ; CADANGAP00001212 ; CADANGAG00001254 .
GeneIDi 4977321.
KEGGi ang:ANI_1_1702014.

Phylogenomic databases

eggNOGi NOG300315.
HOGENOMi HOG000181987.
KOi K01230.
OrthoDBi EOG7BP8BH.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Family and domain databases

Gene3Di 1.50.10.50. 1 hit.
InterProi IPR001382. Glyco_hydro_47.
[Graphical view ]
PANTHERi PTHR11742. PTHR11742. 1 hit.
Pfami PF01532. Glyco_hydro_47. 1 hit.
[Graphical view ]
PRINTSi PR00747. GLYHDRLASE47.
SUPFAMi SSF48225. SSF48225. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88."
    Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G., Schaap P.J., Turner G., de Vries R.P., Albang R., Albermann K., Andersen M.R., Bendtsen J.D., Benen J.A.E., van den Berg M., Breestraat S., Caddick M.X., Contreras R., Cornell M.
    , Coutinho P.M., Danchin E.G.J., Debets A.J.M., Dekker P., van Dijck P.W.M., van Dijk A., Dijkhuizen L., Driessen A.J.M., d'Enfert C., Geysens S., Goosen C., Groot G.S.P., de Groot P.W.J., Guillemette T., Henrissat B., Herweijer M., van den Hombergh J.P.T.W., van den Hondel C.A.M.J.J., van der Heijden R.T.J.M., van der Kaaij R.M., Klis F.M., Kools H.J., Kubicek C.P., van Kuyk P.A., Lauber J., Lu X., van der Maarel M.J.E.C., Meulenberg R., Menke H., Mortimer M.A., Nielsen J., Oliver S.G., Olsthoorn M., Pal K., van Peij N.N.M.E., Ram A.F.J., Rinas U., Roubos J.A., Sagt C.M.J., Schmoll M., Sun J., Ussery D., Varga J., Vervecken W., van de Vondervoort P.J.J., Wedler H., Woesten H.A.B., Zeng A.-P., van Ooyen A.J.J., Visser J., Stam H.
    Nat. Biotechnol. 25:221-231(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CBS 513.88 / FGSC A1513.

Entry informationi

Entry nameiMNS1B_ASPNC
AccessioniPrimary (citable) accession number: A2QAS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: March 6, 2007
Last modified: November 13, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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