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A2QAS2 (MNS1B_ASPNC) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B

EC=3.2.1.113
Alternative name(s):
Class I alpha-mannosidase 1B
Man(9)-alpha-mannosidase 1B
Gene names
Name:mns1B
Synonyms:msdS
ORF Names:An01g12550
OrganismAspergillus niger (strain CBS 513.88 / FGSC A1513) [Complete proteome]
Taxonomic identifier425011 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length513 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the maturation of Asn-linked oligosaccharides. Progressively trims alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2 By similarity.

Catalytic activity

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

Cofactor

Ca2+. Can also use Mg2+, but with lower efficiency By similarity.

Pathway

Protein modification; protein glycosylation.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasmic vesicle lumen By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 47 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 513492Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B
PRO_5000219468

Sites

Active site3771Proton donor By similarity

Amino acid modifications

Glycosylation971N-linked (GlcNAc...) Potential
Glycosylation1171N-linked (GlcNAc...) Potential
Glycosylation1841N-linked (GlcNAc...) Potential
Glycosylation2511N-linked (GlcNAc...) Potential
Glycosylation3221N-linked (GlcNAc...) Potential
Glycosylation3481N-linked (GlcNAc...) Potential
Glycosylation3681N-linked (GlcNAc...) Potential
Disulfide bond334 ↔ 363 By similarity

Sequences

Sequence LengthMass (Da)Tools
A2QAS2 [UniParc].

Last modified March 6, 2007. Version 1.
Checksum: 92F9FBA75F09C927

FASTA51355,929
        10         20         30         40         50         60 
MHLSSLSLSL TALAIVSPSA AYPHLGSSQP VLHTNSDTTQ SRADAIKAAF SHAWDGYLQY 

        70         80         90        100        110        120 
AFPHDELHPV SNGYGDSRNG WGASAVDALS TAVIMRNATI VNQILDHVAK IDYSKTNTTV 

       130        140        150        160        170        180 
SLFETTIRYL GGMLSGYDLL KGPVSDLVQD SSKIDVLLTQ SKNLGDVLKF AFDTPSGVPY 

       190        200        210        220        230        240 
NNLNITSGGN DGAKTNGLAV TGTLALEWTR LSDLTGDTTY ADLSQKAESY LLNPQPKSAE 

       250        260        270        280        290        300 
PFPGLVGSNI NISNGQFTDA QVSWNGGDDS YYEYLIKMYV YDPKRFGLYK DRWVAAAQST 

       310        320        330        340        350        360 
MQHLASHPST RPDLTFLASY NNGTLGLSSQ HLTCFDGGSF LLGGTVLNRT DFINFGLDLV 

       370        380        390        400        410        420 
SGCHDTYNST LTGIGPESFS WDTSDIPSSQ QSLYEKAGFY ITSGAYILRP EVIESFYYAW 

       430        440        450        460        470        480 
RATGQETYRE WIWSAFSAVN DYCRTDSGFS GLTDVNAANG GSRYDNQESF LFAEVMKYSY 

       490        500        510 
MAFAEDAAWQ VQPGSGNQFV FNTEAHPVRV SST 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM269984 Genomic DNA. Translation: CAK37306.1.
RefSeqXP_001389661.1. XM_001389624.2.

3D structure databases

ProteinModelPortalA2QAS2.
SMRA2QAS2. Positions 38-511.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5061.CADANGAP00001212.

Protein family/group databases

CAZyGH47. Glycoside Hydrolase Family 47.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADANGAT00001254; CADANGAP00001212; CADANGAG00001254.
GeneID4977321.
KEGGang:ANI_1_1702014.

Phylogenomic databases

eggNOGNOG300315.
HOGENOMHOG000181987.
KOK01230.
OrthoDBEOG7BP8BH.

Enzyme and pathway databases

UniPathwayUPA00378.

Family and domain databases

Gene3D1.50.10.50. 1 hit.
InterProIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERPTHR11742. PTHR11742. 1 hit.
PfamPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSPR00747. GLYHDRLASE47.
SUPFAMSSF48225. SSF48225. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMNS1B_ASPNC
AccessionPrimary (citable) accession number: A2QAS2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: March 6, 2007
Last modified: November 13, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries