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A2QAS2

- MNS1B_ASPNC

UniProt

A2QAS2 - MNS1B_ASPNC

Protein

Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B

Gene

mns1B

Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 42 (01 Oct 2014)
      Sequence version 1 (06 Mar 2007)
      Previous versions | rss
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    Functioni

    Involved in the maturation of Asn-linked oligosaccharides. Progressively trims alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2 By similarity.By similarity

    Catalytic activityi

    Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

    Cofactori

    Ca2+. Can also use Mg2+, but with lower efficiency By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei377 – 3771Proton donorBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular protein catabolic process Source: ASPGD
    2. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGH47. Glycoside Hydrolase Family 47.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B (EC:3.2.1.113)
    Alternative name(s):
    Class I alpha-mannosidase 1B
    Man(9)-alpha-mannosidase 1B
    Gene namesi
    Name:mns1B
    Synonyms:msdS
    ORF Names:An01g12550
    OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
    Taxonomic identifieri425011 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000006706: Chromosome 2R

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasmic membrane-bounded vesicle lumen Source: UniProtKB-SubCell
    2. membrane Source: InterPro

    Keywords - Cellular componenti

    Cytoplasmic vesicle

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 513492Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1BPRO_5000219468Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi97 – 971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi117 – 1171N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi184 – 1841N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi251 – 2511N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi334 ↔ 363By similarity
    Glycosylationi348 – 3481N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi368 – 3681N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi5061.CADANGAP00001212.

    Structurei

    3D structure databases

    ProteinModelPortaliA2QAS2.
    SMRiA2QAS2. Positions 38-511.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 47 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG300315.
    HOGENOMiHOG000181987.
    KOiK01230.
    OrthoDBiEOG7BP8BH.

    Family and domain databases

    Gene3Di1.50.10.50. 1 hit.
    InterProiIPR001382. Glyco_hydro_47.
    [Graphical view]
    PANTHERiPTHR11742. PTHR11742. 1 hit.
    PfamiPF01532. Glyco_hydro_47. 1 hit.
    [Graphical view]
    PRINTSiPR00747. GLYHDRLASE47.
    SUPFAMiSSF48225. SSF48225. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A2QAS2-1 [UniParc]FASTAAdd to Basket

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    MHLSSLSLSL TALAIVSPSA AYPHLGSSQP VLHTNSDTTQ SRADAIKAAF    50
    SHAWDGYLQY AFPHDELHPV SNGYGDSRNG WGASAVDALS TAVIMRNATI 100
    VNQILDHVAK IDYSKTNTTV SLFETTIRYL GGMLSGYDLL KGPVSDLVQD 150
    SSKIDVLLTQ SKNLGDVLKF AFDTPSGVPY NNLNITSGGN DGAKTNGLAV 200
    TGTLALEWTR LSDLTGDTTY ADLSQKAESY LLNPQPKSAE PFPGLVGSNI 250
    NISNGQFTDA QVSWNGGDDS YYEYLIKMYV YDPKRFGLYK DRWVAAAQST 300
    MQHLASHPST RPDLTFLASY NNGTLGLSSQ HLTCFDGGSF LLGGTVLNRT 350
    DFINFGLDLV SGCHDTYNST LTGIGPESFS WDTSDIPSSQ QSLYEKAGFY 400
    ITSGAYILRP EVIESFYYAW RATGQETYRE WIWSAFSAVN DYCRTDSGFS 450
    GLTDVNAANG GSRYDNQESF LFAEVMKYSY MAFAEDAAWQ VQPGSGNQFV 500
    FNTEAHPVRV SST 513
    Length:513
    Mass (Da):55,929
    Last modified:March 6, 2007 - v1
    Checksum:i92F9FBA75F09C927
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM269984 Genomic DNA. Translation: CAK37306.1.
    RefSeqiXP_001389661.1. XM_001389624.2.

    Genome annotation databases

    EnsemblFungiiCADANGAT00001254; CADANGAP00001212; CADANGAG00001254.
    GeneIDi4977321.
    KEGGiang:ANI_1_1702014.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM269984 Genomic DNA. Translation: CAK37306.1 .
    RefSeqi XP_001389661.1. XM_001389624.2.

    3D structure databases

    ProteinModelPortali A2QAS2.
    SMRi A2QAS2. Positions 38-511.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5061.CADANGAP00001212.

    Protein family/group databases

    CAZyi GH47. Glycoside Hydrolase Family 47.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADANGAT00001254 ; CADANGAP00001212 ; CADANGAG00001254 .
    GeneIDi 4977321.
    KEGGi ang:ANI_1_1702014.

    Phylogenomic databases

    eggNOGi NOG300315.
    HOGENOMi HOG000181987.
    KOi K01230.
    OrthoDBi EOG7BP8BH.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Family and domain databases

    Gene3Di 1.50.10.50. 1 hit.
    InterProi IPR001382. Glyco_hydro_47.
    [Graphical view ]
    PANTHERi PTHR11742. PTHR11742. 1 hit.
    Pfami PF01532. Glyco_hydro_47. 1 hit.
    [Graphical view ]
    PRINTSi PR00747. GLYHDRLASE47.
    SUPFAMi SSF48225. SSF48225. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88."
      Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G., Schaap P.J., Turner G., de Vries R.P., Albang R., Albermann K., Andersen M.R., Bendtsen J.D., Benen J.A.E., van den Berg M., Breestraat S., Caddick M.X., Contreras R., Cornell M.
      , Coutinho P.M., Danchin E.G.J., Debets A.J.M., Dekker P., van Dijck P.W.M., van Dijk A., Dijkhuizen L., Driessen A.J.M., d'Enfert C., Geysens S., Goosen C., Groot G.S.P., de Groot P.W.J., Guillemette T., Henrissat B., Herweijer M., van den Hombergh J.P.T.W., van den Hondel C.A.M.J.J., van der Heijden R.T.J.M., van der Kaaij R.M., Klis F.M., Kools H.J., Kubicek C.P., van Kuyk P.A., Lauber J., Lu X., van der Maarel M.J.E.C., Meulenberg R., Menke H., Mortimer M.A., Nielsen J., Oliver S.G., Olsthoorn M., Pal K., van Peij N.N.M.E., Ram A.F.J., Rinas U., Roubos J.A., Sagt C.M.J., Schmoll M., Sun J., Ussery D., Varga J., Vervecken W., van de Vondervoort P.J.J., Wedler H., Woesten H.A.B., Zeng A.-P., van Ooyen A.J.J., Visser J., Stam H.
      Nat. Biotechnol. 25:221-231(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CBS 513.88 / FGSC A1513.

    Entry informationi

    Entry nameiMNS1B_ASPNC
    AccessioniPrimary (citable) accession number: A2QAS2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 15, 2010
    Last sequence update: March 6, 2007
    Last modified: October 1, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3