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Protein

Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B

Gene

mns1B

Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in the maturation of Asn-linked oligosaccharides. Progressively trims alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2 (By similarity).By similarity

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.By similarity

Cofactori

Ca2+By similarity, Mg2+By similarityNote: Ca2+. Can also use Mg2+, but with lower efficiency.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei377Proton donorBy similarity1
Metal bindingi503CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B (EC:3.2.1.113By similarity)
Alternative name(s):
Class I alpha-mannosidase 1B
Man(9)-alpha-mannosidase 1B
Gene namesi
Name:mns1B
Synonyms:msdS
ORF Names:An01g12550
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006706 Componenti: Chromosome 2R

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_500021946822 – 513Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1BAdd BLAST492

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi97N-linked (GlcNAc...)Sequence analysis1
Glycosylationi117N-linked (GlcNAc...)Sequence analysis1
Glycosylationi184N-linked (GlcNAc...)Sequence analysis1
Glycosylationi251N-linked (GlcNAc...)Sequence analysis1
Glycosylationi322N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi334 ↔ 363By similarity
Glycosylationi348N-linked (GlcNAc...)Sequence analysis1
Glycosylationi368N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliA2QAS2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000181987.
KOiK01230.
OrthoDBiEOG092C1PJZ.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2QAS2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLSSLSLSL TALAIVSPSA AYPHLGSSQP VLHTNSDTTQ SRADAIKAAF
60 70 80 90 100
SHAWDGYLQY AFPHDELHPV SNGYGDSRNG WGASAVDALS TAVIMRNATI
110 120 130 140 150
VNQILDHVAK IDYSKTNTTV SLFETTIRYL GGMLSGYDLL KGPVSDLVQD
160 170 180 190 200
SSKIDVLLTQ SKNLGDVLKF AFDTPSGVPY NNLNITSGGN DGAKTNGLAV
210 220 230 240 250
TGTLALEWTR LSDLTGDTTY ADLSQKAESY LLNPQPKSAE PFPGLVGSNI
260 270 280 290 300
NISNGQFTDA QVSWNGGDDS YYEYLIKMYV YDPKRFGLYK DRWVAAAQST
310 320 330 340 350
MQHLASHPST RPDLTFLASY NNGTLGLSSQ HLTCFDGGSF LLGGTVLNRT
360 370 380 390 400
DFINFGLDLV SGCHDTYNST LTGIGPESFS WDTSDIPSSQ QSLYEKAGFY
410 420 430 440 450
ITSGAYILRP EVIESFYYAW RATGQETYRE WIWSAFSAVN DYCRTDSGFS
460 470 480 490 500
GLTDVNAANG GSRYDNQESF LFAEVMKYSY MAFAEDAAWQ VQPGSGNQFV
510
FNTEAHPVRV SST
Length:513
Mass (Da):55,929
Last modified:March 6, 2007 - v1
Checksum:i92F9FBA75F09C927
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM269984 Genomic DNA. Translation: CAK37306.1.
RefSeqiXP_001389661.1. XM_001389624.2.

Genome annotation databases

EnsemblFungiiCAK37306; CAK37306; An01g12550.
GeneIDi4977321.
KEGGiang:ANI_1_1702014.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM269984 Genomic DNA. Translation: CAK37306.1.
RefSeqiXP_001389661.1. XM_001389624.2.

3D structure databases

ProteinModelPortaliA2QAS2.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAK37306; CAK37306; An01g12550.
GeneIDi4977321.
KEGGiang:ANI_1_1702014.

Phylogenomic databases

HOGENOMiHOG000181987.
KOiK01230.
OrthoDBiEOG092C1PJZ.

Enzyme and pathway databases

UniPathwayiUPA00378.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMNS1B_ASPNC
AccessioniPrimary (citable) accession number: A2QAS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: March 6, 2007
Last modified: November 30, 2016
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.