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A2QAS2

- MNS1B_ASPNC

UniProt

A2QAS2 - MNS1B_ASPNC

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Protein

Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B

Gene

mns1B

Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Involved in the maturation of Asn-linked oligosaccharides. Progressively trims alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2 (By similarity).By similarity

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

Cofactori

Ca2+By similarity, Mg2+By similarityNote: Ca(2+). Can also use Mg(2+), but with lower efficiency.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei377 – 3771Proton donorBy similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular protein catabolic process Source: ASPGD
  2. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B (EC:3.2.1.113)
Alternative name(s):
Class I alpha-mannosidase 1B
Man(9)-alpha-mannosidase 1B
Gene namesi
Name:mns1B
Synonyms:msdS
ORF Names:An01g12550
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006706: Chromosome 2R

Subcellular locationi

GO - Cellular componenti

  1. cytoplasmic vesicle Source: UniProtKB-KW
  2. membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 513492Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1BPRO_5000219468Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi97 – 971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi117 – 1171N-linked (GlcNAc...)Sequence Analysis
Glycosylationi184 – 1841N-linked (GlcNAc...)Sequence Analysis
Glycosylationi251 – 2511N-linked (GlcNAc...)Sequence Analysis
Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi334 ↔ 363By similarity
Glycosylationi348 – 3481N-linked (GlcNAc...)Sequence Analysis
Glycosylationi368 – 3681N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi5061.CADANGAP00001212.

Structurei

3D structure databases

ProteinModelPortaliA2QAS2.
SMRiA2QAS2. Positions 38-511.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG300315.
HOGENOMiHOG000181987.
KOiK01230.
OrthoDBiEOG7BP8BH.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERiPTHR11742. PTHR11742. 1 hit.
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2QAS2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHLSSLSLSL TALAIVSPSA AYPHLGSSQP VLHTNSDTTQ SRADAIKAAF
60 70 80 90 100
SHAWDGYLQY AFPHDELHPV SNGYGDSRNG WGASAVDALS TAVIMRNATI
110 120 130 140 150
VNQILDHVAK IDYSKTNTTV SLFETTIRYL GGMLSGYDLL KGPVSDLVQD
160 170 180 190 200
SSKIDVLLTQ SKNLGDVLKF AFDTPSGVPY NNLNITSGGN DGAKTNGLAV
210 220 230 240 250
TGTLALEWTR LSDLTGDTTY ADLSQKAESY LLNPQPKSAE PFPGLVGSNI
260 270 280 290 300
NISNGQFTDA QVSWNGGDDS YYEYLIKMYV YDPKRFGLYK DRWVAAAQST
310 320 330 340 350
MQHLASHPST RPDLTFLASY NNGTLGLSSQ HLTCFDGGSF LLGGTVLNRT
360 370 380 390 400
DFINFGLDLV SGCHDTYNST LTGIGPESFS WDTSDIPSSQ QSLYEKAGFY
410 420 430 440 450
ITSGAYILRP EVIESFYYAW RATGQETYRE WIWSAFSAVN DYCRTDSGFS
460 470 480 490 500
GLTDVNAANG GSRYDNQESF LFAEVMKYSY MAFAEDAAWQ VQPGSGNQFV
510
FNTEAHPVRV SST
Length:513
Mass (Da):55,929
Last modified:March 6, 2007 - v1
Checksum:i92F9FBA75F09C927
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM269984 Genomic DNA. Translation: CAK37306.1.
RefSeqiXP_001389661.1. XM_001389624.2.

Genome annotation databases

EnsemblFungiiCADANGAT00001254; CADANGAP00001212; CADANGAG00001254.
GeneIDi4977321.
KEGGiang:ANI_1_1702014.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM269984 Genomic DNA. Translation: CAK37306.1 .
RefSeqi XP_001389661.1. XM_001389624.2.

3D structure databases

ProteinModelPortali A2QAS2.
SMRi A2QAS2. Positions 38-511.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5061.CADANGAP00001212.

Protein family/group databases

CAZyi GH47. Glycoside Hydrolase Family 47.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CADANGAT00001254 ; CADANGAP00001212 ; CADANGAG00001254 .
GeneIDi 4977321.
KEGGi ang:ANI_1_1702014.

Phylogenomic databases

eggNOGi NOG300315.
HOGENOMi HOG000181987.
KOi K01230.
OrthoDBi EOG7BP8BH.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Family and domain databases

Gene3Di 1.50.10.50. 1 hit.
InterProi IPR001382. Glyco_hydro_47.
[Graphical view ]
PANTHERi PTHR11742. PTHR11742. 1 hit.
Pfami PF01532. Glyco_hydro_47. 1 hit.
[Graphical view ]
PRINTSi PR00747. GLYHDRLASE47.
SUPFAMi SSF48225. SSF48225. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88."
    Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G., Schaap P.J., Turner G., de Vries R.P., Albang R., Albermann K., Andersen M.R., Bendtsen J.D., Benen J.A.E., van den Berg M., Breestraat S., Caddick M.X., Contreras R., Cornell M.
    , Coutinho P.M., Danchin E.G.J., Debets A.J.M., Dekker P., van Dijck P.W.M., van Dijk A., Dijkhuizen L., Driessen A.J.M., d'Enfert C., Geysens S., Goosen C., Groot G.S.P., de Groot P.W.J., Guillemette T., Henrissat B., Herweijer M., van den Hombergh J.P.T.W., van den Hondel C.A.M.J.J., van der Heijden R.T.J.M., van der Kaaij R.M., Klis F.M., Kools H.J., Kubicek C.P., van Kuyk P.A., Lauber J., Lu X., van der Maarel M.J.E.C., Meulenberg R., Menke H., Mortimer M.A., Nielsen J., Oliver S.G., Olsthoorn M., Pal K., van Peij N.N.M.E., Ram A.F.J., Rinas U., Roubos J.A., Sagt C.M.J., Schmoll M., Sun J., Ussery D., Varga J., Vervecken W., van de Vondervoort P.J.J., Wedler H., Woesten H.A.B., Zeng A.-P., van Ooyen A.J.J., Visser J., Stam H.
    Nat. Biotechnol. 25:221-231(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CBS 513.88 / FGSC A1513.

Entry informationi

Entry nameiMNS1B_ASPNC
AccessioniPrimary (citable) accession number: A2QAS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: March 6, 2007
Last modified: November 26, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3