Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B precursor - Aspergillus niger (strain CBS 513.88 / FGSC A1513)

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A2QAS2 (MNS1B_ASPNC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 38. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B
EC=3.2.1.113

Alternative name(s):
Class I alpha-mannosidase 1B
Man(9)-alpha-mannosidase 1B

Gene names

Name:	mns1B
Synonyms:	msdS
ORF Names:	An01g12550

Organism

Aspergillus niger (strain CBS 513.88 / FGSC A1513) [Complete proteome]

Taxonomic identifier

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus ›

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the maturation of Asn-linked oligosaccharides. Progressively trims alpha-1,2-linked mannose residues from Man₉GlcNAc₂ to produce Man₅GlcNAc₂ By similarity.
Catalytic activity	Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man₉(GlcNAc)₂.
Cofactor	Ca²⁺. Can also use Mg²⁺, but with lower efficiency By similarity.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasmic vesicle lumen By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 47 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasmic vesicle
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasmic membrane-bounded vesicle lumen Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

21

Potential

Chain

492

Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B

PRO_5000219468

Sites

Active site

1

Proton donor By similarity

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Disulfide bond

By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

A2QAS2 [UniParc].

Last modified March 6, 2007. Version 1.
Checksum: 92F9FBA75F09C927
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513

55,929

        10         20         30         40         50         60 
MHLSSLSLSL TALAIVSPSA AYPHLGSSQP VLHTNSDTTQ SRADAIKAAF SHAWDGYLQY 

        70         80         90        100        110        120 
AFPHDELHPV SNGYGDSRNG WGASAVDALS TAVIMRNATI VNQILDHVAK IDYSKTNTTV 

       130        140        150        160        170        180 
SLFETTIRYL GGMLSGYDLL KGPVSDLVQD SSKIDVLLTQ SKNLGDVLKF AFDTPSGVPY 

       190        200        210        220        230        240 
NNLNITSGGN DGAKTNGLAV TGTLALEWTR LSDLTGDTTY ADLSQKAESY LLNPQPKSAE 

       250        260        270        280        290        300 
PFPGLVGSNI NISNGQFTDA QVSWNGGDDS YYEYLIKMYV YDPKRFGLYK DRWVAAAQST 

       310        320        330        340        350        360 
MQHLASHPST RPDLTFLASY NNGTLGLSSQ HLTCFDGGSF LLGGTVLNRT DFINFGLDLV 

       370        380        390        400        410        420 
SGCHDTYNST LTGIGPESFS WDTSDIPSSQ QSLYEKAGFY ITSGAYILRP EVIESFYYAW 

       430        440        450        460        470        480 
RATGQETYRE WIWSAFSAVN DYCRTDSGFS GLTDVNAANG GSRYDNQESF LFAEVMKYSY 

       490        500        510 
MAFAEDAAWQ VQPGSGNQFV FNTEAHPVRV SST

References

[1]

"Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88."
Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G., Schaap P.J., Turner G., de Vries R.P., Albang R., Albermann K., Andersen M.R., Bendtsen J.D., Benen J.A.E., van den Berg M., Breestraat S., Caddick M.X., Contreras R., Cornell M.

expand/collapse author list

Stam H.
Nat. Biotechnol. 25:221-231(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CBS 513.88 / FGSC A1513.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM269984 Genomic DNA. Translation: CAK37306.1.
RefSeq	XP_001389661.1. XM_001389624.2.
3D structure databases
ProteinModelPortal	A2QAS2.
SMR	A2QAS2. Positions 38-511.
ModBase	Search...
Protein-protein interaction databases
STRING	5061.CADANGAP00001212.
Protein family/group databases
CAZy	GH47. Glycoside Hydrolase Family 47.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADANGAT00001254; CADANGAP00001212; CADANGAG00001254.
GeneID	4977321.
KEGG	ang:ANI_1_1702014.
Phylogenomic databases
eggNOG	NOG300315.
HOGENOM	HOG000181987.
KO	K01230.
OrthoDB	EOG42JS14.
Enzyme and pathway databases
UniPathway	UPA00378.
Family and domain databases
Gene3D	1.50.10.50. 1 hit.
InterPro	IPR001382. Glyco_hydro_47. [Graphical view]
PANTHER	PTHR11742. PTHR11742. 1 hit.
Pfam	PF01532. Glyco_hydro_47. 1 hit. [Graphical view]
PRINTS	PR00747. GLYHDRLASE47.
SUPFAM	SSF48225. Glyco_hydro_47. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MNS1B_ASPNC

Accession

Primary (citable) accession number: A2QAS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	March 6, 2007
Last modified:	May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents