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Protein

Probable beta-galactosidase A

Gene

lacA

Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei96SubstrateBy similarity1
Binding sitei140SubstrateBy similarity1
Binding sitei141Substrate; via amide nitrogenBy similarity1
Binding sitei142SubstrateBy similarity1
Binding sitei199SubstrateBy similarity1
Active sitei200Proton donorSequence analysis1
Binding sitei260SubstrateBy similarity1
Active sitei298NucleophileSequence analysis1
Binding sitei364SubstrateBy similarity1

GO - Molecular functioni

  • beta-galactosidase activity Source: AspGD

GO - Biological processi

  • carbohydrate metabolic process Source: AspGD
  • polysaccharide catabolic process Source: UniProtKB-KW

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase A (EC:3.2.1.23)
Alternative name(s):
Lactase A
Gene namesi
Name:lacA
ORF Names:An01g12150
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006706 Componenti: Chromosome 2R

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_500021945819 – 1007Probable beta-galactosidase AAdd BLAST989

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi156N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi205 ↔ 206By similarity
Disulfide bondi266 ↔ 315By similarity
Glycosylationi373N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi402N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi422N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi478N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi522N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi622N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi739N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi760N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi777N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi805N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi914N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi5061.CADANGAP00001173.

Structurei

Secondary structure

11007
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi44 – 48Combined sources5
Beta strandi53 – 55Combined sources3
Beta strandi58 – 60Combined sources3
Beta strandi62 – 66Combined sources5
Helixi69 – 71Combined sources3
Helixi75 – 86Combined sources12
Turni87 – 89Combined sources3
Beta strandi92 – 96Combined sources5
Helixi99 – 102Combined sources4
Helixi113 – 115Combined sources3
Helixi118 – 127Combined sources10
Beta strandi130 – 137Combined sources8
Helixi144 – 147Combined sources4
Helixi150 – 154Combined sources5
Helixi164 – 183Combined sources20
Helixi186 – 188Combined sources3
Beta strandi190 – 199Combined sources10
Helixi213 – 225Combined sources13
Beta strandi236 – 239Combined sources4
Beta strandi257 – 259Combined sources3
Helixi281 – 288Combined sources8
Beta strandi295 – 302Combined sources8
Helixi312 – 318Combined sources7
Helixi321 – 332Combined sources12
Turni333 – 335Combined sources3
Beta strandi337 – 342Combined sources6
Helixi350 – 352Combined sources3
Helixi377 – 390Combined sources14
Helixi394 – 397Combined sources4
Beta strandi398 – 400Combined sources3
Beta strandi404 – 410Combined sources7
Beta strandi414 – 420Combined sources7
Beta strandi422 – 424Combined sources3
Beta strandi426 – 434Combined sources9
Beta strandi440 – 443Combined sources4
Beta strandi445 – 447Combined sources3
Beta strandi454 – 456Combined sources3
Beta strandi458 – 460Combined sources3
Beta strandi463 – 468Combined sources6
Beta strandi471 – 479Combined sources9
Beta strandi482 – 497Combined sources16
Beta strandi500 – 507Combined sources8
Beta strandi512 – 518Combined sources7
Beta strandi523 – 528Combined sources6
Beta strandi534 – 537Combined sources4
Beta strandi539 – 547Combined sources9
Beta strandi553 – 557Combined sources5
Beta strandi560 – 566Combined sources7
Helixi567 – 570Combined sources4
Beta strandi578 – 583Combined sources6
Helixi590 – 594Combined sources5
Beta strandi598 – 600Combined sources3
Beta strandi602 – 611Combined sources10
Beta strandi614 – 623Combined sources10
Beta strandi625 – 631Combined sources7
Beta strandi638 – 641Combined sources4
Beta strandi644 – 646Combined sources3
Beta strandi656 – 660Combined sources5
Helixi672 – 674Combined sources3
Beta strandi678 – 682Combined sources5
Helixi684 – 686Combined sources3
Beta strandi706 – 708Combined sources3
Beta strandi711 – 714Combined sources4
Helixi718 – 721Combined sources4
Beta strandi728 – 735Combined sources8
Beta strandi742 – 748Combined sources7
Beta strandi755 – 759Combined sources5
Beta strandi762 – 767Combined sources6
Beta strandi774 – 781Combined sources8
Beta strandi790 – 797Combined sources8
Helixi812 – 814Combined sources3
Beta strandi818 – 824Combined sources7
Helixi829 – 831Combined sources3
Beta strandi833 – 839Combined sources7
Turni840 – 843Combined sources4
Turni848 – 850Combined sources3
Beta strandi852 – 854Combined sources3
Helixi859 – 862Combined sources4
Turni863 – 866Combined sources4
Beta strandi867 – 869Combined sources3
Beta strandi875 – 877Combined sources3
Turni880 – 882Combined sources3
Beta strandi884 – 899Combined sources16
Beta strandi908 – 912Combined sources5
Beta strandi921 – 927Combined sources7
Beta strandi930 – 936Combined sources7
Turni937 – 939Combined sources3
Beta strandi944 – 947Combined sources4
Beta strandi955 – 966Combined sources12
Beta strandi977 – 981Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IFPX-ray1.71A1-1007[»]
5IFTX-ray2.45A1-1007[»]
5IHRX-ray2.40A1-1007[»]
5JUVX-ray2.27A1-1007[»]
5MGCX-ray2.30A1-1007[»]
5MGDX-ray2.15A1-1007[»]
ProteinModelPortaliA2QAN3.
SMRiA2QAN3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000181922.
OrthoDBiEOG092C0SLI.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
InterProiView protein in InterPro
IPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR019801. Glyco_hydro_35_CS.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiView protein in Pfam
PF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
PRINTSiPR00742. GLHYDRLASE35.
SMARTiView protein in SMART
SM01029. BetaGal_dom2. 1 hit.
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiView protein in PROSITE
PS01182. GLYCOSYL_HYDROL_F35. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2QAN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSSACAIA LLAAQAAGAS IKHRINGFTL TEHSDPAKRE LLQKYVTWDD
60 70 80 90 100
KSLFINGERI MIFSGEFHPF RLPVKELQLD IFQKVKALGF NCVSFYVDWA
110 120 130 140 150
LVEGKPGEYR ADGIFDLEPF FDAASEAGIY LLARPGPYIN AESSGGGFPG
160 170 180 190 200
WLQRVNGTLR SSDKAYLDAT DNYVSHVAAT IAKYQITNGG PIILYQPENE
210 220 230 240 250
YTSGCCGVEF PDPVYMQYVE DQARNAGVVI PLINNDASAS GNNAPGTGKG
260 270 280 290 300
AVDIYGHDSY PLGFDCANPT VWPSGDLPTN FRTLHLEQSP TTPYAIVEFQ
310 320 330 340 350
GGSYDPWGGP GFAACSELLN NEFERVFYKN DFSFQIAIMN LYMIFGGTNW
360 370 380 390 400
GNLGYPNGYT SYDYGSAVTE SRNITREKYS ELKLLGNFAK VSPGYLTASP
410 420 430 440 450
GNLTTSGYAD TTDLTVTPLL GNSTGSFFVV RHSDYSSEES TSYKLRLPTS
460 470 480 490 500
AGSVTIPQLG GTLTLNGRDS KIHVTDYNVS GTNIIYSTAE VFTWKKFADG
510 520 530 540 550
KVLVLYGGAG EHHELAISTK SNVTVIEGSE SGISSKQTSS SVVVGWDVST
560 570 580 590 600
TRRIIQVGDL KILLLDRNSA YNYWVPQLAT DGTSPGFSTP EKVASSIIVK
610 620 630 640 650
AGYLVRTAYL KGSGLYLTAD FNATTSVEVI GVPSTAKNLF INGDKTSHTV
660 670 680 690 700
DKNGIWSATV DYNAPDISLP SLKDLDWKYV DTLPEIQSSY DDSLWPAADL
710 720 730 740 750
KQTKNTLRSL TTPTSLYSSD YGFHTGYLLY RGHFTATGNE STFAIDTQGG
760 770 780 790 800
SAFGSSVWLN GTYLGSWTGL YANSDYNATY NLPQLQAGKT YVITVVIDNM
810 820 830 840 850
GLEENWTVGE DLMKTPRGIL NFLLAGRPSS AISWKLTGNL GGEDYEDKVR
860 870 880 890 900
GPLNEGGLYA ERQGFHQPEP PSQNWKSSSP LEGLSEAGIG FYSASFDLDL
910 920 930 940 950
PKGWDVPLFL NIGNSTTPSP YRVQVYVNGY QYAKYISNIG PQTSFPVPEG
960 970 980 990 1000
ILNYRGTNWL AVTLWALDSA GGKLESLELS YTTPVLTALG EVESVDQPKY

KKRKGAY
Length:1,007
Mass (Da):109,712
Last modified:March 6, 2007 - v1
Checksum:iCD1E91854D7A8A0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM269982 Genomic DNA. Translation: CAK44114.1.
RefSeqiXP_001389622.1. XM_001389585.2.

Genome annotation databases

EnsemblFungiiCAK44114; CAK44114; An01g12150.
GeneIDi4977988.
KEGGiang:ANI_1_1636014.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiBGALA_ASPNC
AccessioniPrimary (citable) accession number: A2QAN3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: March 6, 2007
Last modified: July 5, 2017
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families