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Protein

Probable beta-galactosidase A

Gene

lacA

Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei96SubstrateBy similarity1
Binding sitei140SubstrateBy similarity1
Binding sitei141Substrate; via amide nitrogenBy similarity1
Binding sitei142SubstrateBy similarity1
Binding sitei199SubstrateBy similarity1
Active sitei200Proton donorSequence analysis1
Binding sitei260SubstrateBy similarity1
Active sitei298NucleophileSequence analysis1
Binding sitei364SubstrateBy similarity1

GO - Molecular functioni

  • beta-galactosidase activity Source: ASPGD

GO - Biological processi

  • carbohydrate metabolic process Source: ASPGD
  • polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase A (EC:3.2.1.23)
Alternative name(s):
Lactase A
Gene namesi
Name:lacA
ORF Names:An01g12150
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006706 Componenti: Chromosome 2R

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_500021945819 – 1007Probable beta-galactosidase AAdd BLAST989

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi156N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi205 ↔ 206By similarity
Disulfide bondi266 ↔ 315By similarity
Glycosylationi373N-linked (GlcNAc...)Sequence analysis1
Glycosylationi402N-linked (GlcNAc...)Sequence analysis1
Glycosylationi422N-linked (GlcNAc...)Sequence analysis1
Glycosylationi478N-linked (GlcNAc...)Sequence analysis1
Glycosylationi522N-linked (GlcNAc...)Sequence analysis1
Glycosylationi622N-linked (GlcNAc...)Sequence analysis1
Glycosylationi739N-linked (GlcNAc...)Sequence analysis1
Glycosylationi760N-linked (GlcNAc...)Sequence analysis1
Glycosylationi777N-linked (GlcNAc...)Sequence analysis1
Glycosylationi805N-linked (GlcNAc...)Sequence analysis1
Glycosylationi914N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliA2QAN3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000181922.
OrthoDBiEOG092C0SLI.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 2 hits.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2QAN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSSACAIA LLAAQAAGAS IKHRINGFTL TEHSDPAKRE LLQKYVTWDD
60 70 80 90 100
KSLFINGERI MIFSGEFHPF RLPVKELQLD IFQKVKALGF NCVSFYVDWA
110 120 130 140 150
LVEGKPGEYR ADGIFDLEPF FDAASEAGIY LLARPGPYIN AESSGGGFPG
160 170 180 190 200
WLQRVNGTLR SSDKAYLDAT DNYVSHVAAT IAKYQITNGG PIILYQPENE
210 220 230 240 250
YTSGCCGVEF PDPVYMQYVE DQARNAGVVI PLINNDASAS GNNAPGTGKG
260 270 280 290 300
AVDIYGHDSY PLGFDCANPT VWPSGDLPTN FRTLHLEQSP TTPYAIVEFQ
310 320 330 340 350
GGSYDPWGGP GFAACSELLN NEFERVFYKN DFSFQIAIMN LYMIFGGTNW
360 370 380 390 400
GNLGYPNGYT SYDYGSAVTE SRNITREKYS ELKLLGNFAK VSPGYLTASP
410 420 430 440 450
GNLTTSGYAD TTDLTVTPLL GNSTGSFFVV RHSDYSSEES TSYKLRLPTS
460 470 480 490 500
AGSVTIPQLG GTLTLNGRDS KIHVTDYNVS GTNIIYSTAE VFTWKKFADG
510 520 530 540 550
KVLVLYGGAG EHHELAISTK SNVTVIEGSE SGISSKQTSS SVVVGWDVST
560 570 580 590 600
TRRIIQVGDL KILLLDRNSA YNYWVPQLAT DGTSPGFSTP EKVASSIIVK
610 620 630 640 650
AGYLVRTAYL KGSGLYLTAD FNATTSVEVI GVPSTAKNLF INGDKTSHTV
660 670 680 690 700
DKNGIWSATV DYNAPDISLP SLKDLDWKYV DTLPEIQSSY DDSLWPAADL
710 720 730 740 750
KQTKNTLRSL TTPTSLYSSD YGFHTGYLLY RGHFTATGNE STFAIDTQGG
760 770 780 790 800
SAFGSSVWLN GTYLGSWTGL YANSDYNATY NLPQLQAGKT YVITVVIDNM
810 820 830 840 850
GLEENWTVGE DLMKTPRGIL NFLLAGRPSS AISWKLTGNL GGEDYEDKVR
860 870 880 890 900
GPLNEGGLYA ERQGFHQPEP PSQNWKSSSP LEGLSEAGIG FYSASFDLDL
910 920 930 940 950
PKGWDVPLFL NIGNSTTPSP YRVQVYVNGY QYAKYISNIG PQTSFPVPEG
960 970 980 990 1000
ILNYRGTNWL AVTLWALDSA GGKLESLELS YTTPVLTALG EVESVDQPKY

KKRKGAY
Length:1,007
Mass (Da):109,712
Last modified:March 6, 2007 - v1
Checksum:iCD1E91854D7A8A0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM269982 Genomic DNA. Translation: CAK44114.1.
RefSeqiXP_001389622.1. XM_001389585.2.

Genome annotation databases

EnsemblFungiiCAK44114; CAK44114; An01g12150.
GeneIDi4977988.
KEGGiang:ANI_1_1636014.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM269982 Genomic DNA. Translation: CAK44114.1.
RefSeqiXP_001389622.1. XM_001389585.2.

3D structure databases

ProteinModelPortaliA2QAN3.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAK44114; CAK44114; An01g12150.
GeneIDi4977988.
KEGGiang:ANI_1_1636014.

Phylogenomic databases

HOGENOMiHOG000181922.
OrthoDBiEOG092C0SLI.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 2 hits.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGALA_ASPNC
AccessioniPrimary (citable) accession number: A2QAN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: March 6, 2007
Last modified: November 30, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.