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Protein

Probable endopolygalacturonase I

Gene

pgaI

Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall (By similarity).By similarity

Catalytic activityi

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei207 – 2071Proton donorPROSITE-ProRule annotation
Active sitei229 – 2291PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable endopolygalacturonase I (EC:3.2.1.15)
Alternative name(s):
Pectinase 1
Polygalacturonase I
Short name:
PG-I
Gene namesi
Name:pgaI
Synonyms:pg1, pga1
ORF Names:An01g11520
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006706 Componenti: Chromosome 2R

Organism-specific databases

EuPathDBiFungiDB:An01g11520.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei8269. Asp n Pectinase.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence analysisAdd
BLAST
Propeptidei19 – 3113Sequence analysisPRO_0000393621Add
BLAST
Chaini32 – 368337Probable endopolygalacturonase IPRO_5000219447Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi35 ↔ 50By similarity
Disulfide bondi209 ↔ 225By similarity
Glycosylationi246 – 2461N-linked (GlcNAc...)Sequence analysis
Disulfide bondi335 ↔ 340By similarity
Disulfide bondi359 ↔ 368By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Structurei

3D structure databases

ProteinModelPortaliA2QAH3.
SMRiA2QAH3. Positions 33-368.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati140 – 16122PbH1 1Add
BLAST
Repeati162 – 19231PbH1 2Add
BLAST
Repeati193 – 21422PbH1 3Add
BLAST
Repeati244 – 26522PbH1 4Add
BLAST
Repeati273 – 29523PbH1 5Add
BLAST
Repeati307 – 35246PbH1 6Add
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated
Contains 6 PbH1 repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000193107.
KOiK01213.
OrthoDBiEOG71VT3J.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 6 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2QAH3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHSYQLLGLA AVGSLVSAAP APSRVSEFAK KASTCTFTSA SEASESISSC
60 70 80 90 100
SDVVLSSIEV PAGETLDLSD AADGSTITFE GTTSFGYKEW KGPLIRFGGK
110 120 130 140 150
DLTVTMADGA VIDGDGSRWW DSKGTNGGKT KPKFMYIHDV EDSTFKGINI
160 170 180 190 200
KNTPVQAISV QATNVHLNDF TIDNSDGDDN GGHNTDGFDI SESTGVYISG
210 220 230 240 250
ATVKNQDDCI AINSGESISF TGGTCSGGHG LSIGSVGGRD DNTVKNVTIS
260 270 280 290 300
DSTVSNSANG VRIKTIYKET GDVSEITYSN IQLSGITDYG IVIEQDYENG
310 320 330 340 350
SPTGTPSTGI PITDVTVDGV TGTLEDDATQ VYILCGDGSC SDWTWSGVDL
360
SGGKTSDKCE NVPSGASC
Length:368
Mass (Da):38,108
Last modified:March 6, 2007 - v1
Checksum:iCD9B46A9A99B5102
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM269981 Genomic DNA. Translation: CAK44054.1.
RefSeqiXP_001389562.1. XM_001389525.1.

Genome annotation databases

EnsemblFungiiCADANGAT00001150; CADANGAP00001113; CADANGAG00001150.
GeneIDi4978020.
KEGGiang:ANI_1_1554014.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM269981 Genomic DNA. Translation: CAK44054.1.
RefSeqiXP_001389562.1. XM_001389525.1.

3D structure databases

ProteinModelPortaliA2QAH3.
SMRiA2QAH3. Positions 33-368.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei8269. Asp n Pectinase.
CAZyiGH28. Glycoside Hydrolase Family 28.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADANGAT00001150; CADANGAP00001113; CADANGAG00001150.
GeneIDi4978020.
KEGGiang:ANI_1_1554014.

Organism-specific databases

EuPathDBiFungiDB:An01g11520.

Phylogenomic databases

HOGENOMiHOG000193107.
KOiK01213.
OrthoDBiEOG71VT3J.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 6 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88."
    Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G., Schaap P.J., Turner G., de Vries R.P., Albang R., Albermann K., Andersen M.R., Bendtsen J.D., Benen J.A.E., van den Berg M., Breestraat S., Caddick M.X., Contreras R., Cornell M.
    , Coutinho P.M., Danchin E.G.J., Debets A.J.M., Dekker P., van Dijck P.W.M., van Dijk A., Dijkhuizen L., Driessen A.J.M., d'Enfert C., Geysens S., Goosen C., Groot G.S.P., de Groot P.W.J., Guillemette T., Henrissat B., Herweijer M., van den Hombergh J.P.T.W., van den Hondel C.A.M.J.J., van der Heijden R.T.J.M., van der Kaaij R.M., Klis F.M., Kools H.J., Kubicek C.P., van Kuyk P.A., Lauber J., Lu X., van der Maarel M.J.E.C., Meulenberg R., Menke H., Mortimer M.A., Nielsen J., Oliver S.G., Olsthoorn M., Pal K., van Peij N.N.M.E., Ram A.F.J., Rinas U., Roubos J.A., Sagt C.M.J., Schmoll M., Sun J., Ussery D., Varga J., Vervecken W., van de Vondervoort P.J.J., Wedler H., Woesten H.A.B., Zeng A.-P., van Ooyen A.J.J., Visser J., Stam H.
    Nat. Biotechnol. 25:221-231(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CBS 513.88 / FGSC A1513.

Entry informationi

Entry nameiPGLR1_ASPNC
AccessioniPrimary (citable) accession number: A2QAH3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: March 6, 2007
Last modified: April 13, 2016
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.