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A2QA64

- BGALB_ASPNC

UniProt

A2QA64 - BGALB_ASPNC

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Protein
Probable beta-galactosidase B
Gene
lacB, An01g10350
Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans By similarity.

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei90 – 901Substrate By similarity
Binding sitei135 – 1351Substrate By similarity
Binding sitei136 – 1361Substrate; via amide nitrogen By similarity
Binding sitei137 – 1371Substrate By similarity
Binding sitei195 – 1951Substrate By similarity
Active sitei196 – 1961Proton donor Reviewed prediction
Binding sitei265 – 2651Substrate By similarity
Active sitei308 – 3081Nucleophile Reviewed prediction
Binding sitei373 – 3731Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase B (EC:3.2.1.23)
Alternative name(s):
Lactase B
Gene namesi
Name:lacB
ORF Names:An01g10350
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006706: Chromosome 2R

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 Reviewed prediction
Add
BLAST
Chaini21 – 1017997Probable beta-galactosidase B
PRO_5000219426Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi23 – 231N-linked (GlcNAc...) Reviewed prediction
Glycosylationi100 – 1001N-linked (GlcNAc...) Reviewed prediction
Glycosylationi158 – 1581N-linked (GlcNAc...) Reviewed prediction
Glycosylationi211 – 2111N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi271 ↔ 324 By similarity
Glycosylationi411 – 4111N-linked (GlcNAc...) Reviewed prediction
Glycosylationi417 – 4171N-linked (GlcNAc...) Reviewed prediction
Glycosylationi456 – 4561N-linked (GlcNAc...) Reviewed prediction
Glycosylationi628 – 6281N-linked (GlcNAc...) Reviewed prediction
Glycosylationi681 – 6811N-linked (GlcNAc...) Reviewed prediction
Glycosylationi737 – 7371N-linked (GlcNAc...) Reviewed prediction
Glycosylationi770 – 7701N-linked (GlcNAc...) Reviewed prediction
Glycosylationi777 – 7771N-linked (GlcNAc...) Reviewed prediction
Glycosylationi785 – 7851N-linked (GlcNAc...) Reviewed prediction
Glycosylationi828 – 8281N-linked (GlcNAc...) Reviewed prediction
Glycosylationi829 – 8291N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiA2QA64.

Interactioni

Protein-protein interaction databases

STRINGi5061.CADANGAP00001004.

Structurei

3D structure databases

ProteinModelPortaliA2QA64.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000181922.
OrthoDBiEOG7ZGXBD.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2QA64-1 [UniParc]FASTAAdd to Basket

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MTRITKLCVL LLSSIGLLAA AQNQTETGWP LHDDGLTTDV QWDHYSFKVH     50
GERIFVFSGE FHYWRIPVPG LWRDILEKIK AAGFTTFAFY SSWAWHAPNN 100
HTVDFSTGAR DITPIFELAK ELGMYIIVRP GPYINAEASA GGFPLWLTTG 150
DYGTLRNNDS RYTEAWKPYF EKMTEITSRY QITNGHNTFC YQIENEYGDQ 200
WLSDPSERVP NETAIAYMEL LESSARENGI LVPFTANDPN MNAMAWSRDW 250
SNAGGNVDVV GLDSYPSCWT CDVSQCTSTN GEYVAYQVVE YYDYFLDFSP 300
TMPSFMPEFQ GGSYNPWAGP EGGCGDDTGV DFVNLFYRWN IAQRVTAMSL 350
YMLYGGTNWG AIAAPVTATS YDYSSPISED RSISSKYYET KLLSLFTRSA 400
RDLTMTDLIG NGTQYTNNTA VKAYELRNPT TNAGFYVTLH EDSTVGTNEA 450
FSLRVNTSAG NLIVPRLGGS IRLNGHQSKI IVTDFTFGSE TLLYSTAEVL 500
TYAVIDKKPT LVLWVPTDES GEFAVKGAKS GSVVSKCQSC PAINFHQQGG 550
NLIVGFTQSQ GMSVVQIDND IRVVLLDRTA AYKFWAPALT EDPLVPEDEA 600
VVLIQGPYLV RSASLEKSTL AIKGDSINET AVEIFAPENV KTITWNGKQL 650
KTSKSSYGSL KATIAAPASI QLPAFTSWKV NDSLPERLPT YDASGPAWVD 700
ANHMTTANPS KPATLPVLYA DEYGFHNGVR LWRGYFNGTA SGVFLNVQGG 750
SAFGFSAYLN GHFLGSYLGN ASIEQANQTF LFPNNITHPT TQNTLLVIHD 800
DTGHDETTGA LNPRGILEAR LLPSDTTNNS TSPEFTHWRI AGTAGGESNL 850
DPVRGAWNED GLYAERVGWH LPGFDDSTWS SVSSSSSLSF TGATVKFFRT 900
TIPLDIPRGL DVSISFVLGT PDNAPNAYRA QLFVNGYQYG RFNPYIGNQV 950
VFPVPVGVLD YTGENTIGVA VWAQTEDGAG ITVDWKVNYV ADSSLDVSGL 1000
ETGELRPGWS AERLKFA 1017
Length:1,017
Mass (Da):111,810
Last modified:July 13, 2010 - v2
Checksum:i3ACF69B3DFD08D04
GO

Sequence cautioni

The sequence CAK37216.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM269980 Genomic DNA. Translation: CAK37216.1. Sequence problems.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM269980 Genomic DNA. Translation: CAK37216.1 . Sequence problems.

3D structure databases

ProteinModelPortali A2QA64.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5061.CADANGAP00001004.

Protein family/group databases

CAZyi GH35. Glycoside Hydrolase Family 35.

Proteomic databases

PRIDEi A2QA64.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG1874.
HOGENOMi HOG000181922.
OrthoDBi EOG7ZGXBD.

Family and domain databases

Gene3Di 2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR23421. PTHR23421. 1 hit.
Pfami PF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view ]
PRINTSi PR00742. GLHYDRLASE35.
SMARTi SM01029. BetaGal_dom2. 1 hit.
[Graphical view ]
SUPFAMi SSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88."
    Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G., Schaap P.J., Turner G., de Vries R.P., Albang R., Albermann K., Andersen M.R., Bendtsen J.D., Benen J.A.E., van den Berg M., Breestraat S., Caddick M.X., Contreras R., Cornell M.
    , Coutinho P.M., Danchin E.G.J., Debets A.J.M., Dekker P., van Dijck P.W.M., van Dijk A., Dijkhuizen L., Driessen A.J.M., d'Enfert C., Geysens S., Goosen C., Groot G.S.P., de Groot P.W.J., Guillemette T., Henrissat B., Herweijer M., van den Hombergh J.P.T.W., van den Hondel C.A.M.J.J., van der Heijden R.T.J.M., van der Kaaij R.M., Klis F.M., Kools H.J., Kubicek C.P., van Kuyk P.A., Lauber J., Lu X., van der Maarel M.J.E.C., Meulenberg R., Menke H., Mortimer M.A., Nielsen J., Oliver S.G., Olsthoorn M., Pal K., van Peij N.N.M.E., Ram A.F.J., Rinas U., Roubos J.A., Sagt C.M.J., Schmoll M., Sun J., Ussery D., Varga J., Vervecken W., van de Vondervoort P.J.J., Wedler H., Woesten H.A.B., Zeng A.-P., van Ooyen A.J.J., Visser J., Stam H.
    Nat. Biotechnol. 25:221-231(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CBS 513.88 / FGSC A1513.

Entry informationi

Entry nameiBGALB_ASPNC
AccessioniPrimary (citable) accession number: A2QA64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: July 13, 2010
Last modified: April 16, 2014
This is version 50 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi