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A2Q9E4 (TRMB_ASPNC) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trm8
ORF Names:An01g07580
OrganismAspergillus niger (strain CBS 513.88 / FGSC A1513) [Complete proteome]
Taxonomic identifier425011 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length297 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subunit structure

Forms a complex with trm82 By similarity. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 297297tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370587

Regions

Region124 – 1252S-adenosyl-L-methionine binding By similarity
Region171 – 1722S-adenosyl-L-methionine binding By similarity
Region270 – 2723S-adenosyl-L-methionine binding By similarity

Sites

Active site1941 By similarity
Binding site1011S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1911S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
A2Q9E4 [UniParc].

Last modified March 6, 2007. Version 1.
Checksum: 84C5A7C435920A92

FASTA29734,052
        10         20         30         40         50         60 
MSQPPAKRQK RAEYRKQAAE AVVAQPDESA PVAHVKLPKK KFYRQRAHAN PFSDHQLNYP 

        70         80         90        100        110        120 
LSPAHMDWAS HFPAFVNPDA TQTNLIGTRK LLKDVEVVDI GCGFGGLLVG LAGLLPETLM 

       130        140        150        160        170        180 
VGMEIRIAVL EYLNTRIQAL RVQQQQQQQK TQSHAALVPG GYQNISAIRS NTMKFFPNFF 

       190        200        210        220        230        240 
NKHQLSKIFI CFPDPHFKAR KHKARIISET LNAEYAYALR PGGLLYTITD VEEYHHWILR 

       250        260        270        280        290 
HFRDESALFE RVSEEELEKD ECVKVMKEAT EEGKKVTRNK GNKYVAVFRR KEDPEWA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM269973 Genomic DNA. Translation: CAK96183.1.

3D structure databases

ProteinModelPortalA2Q9E4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5061.CADANGAP00000734.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADANGAT00000754; CADANGAP00000734; CADANGAG00000754.

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000260965.
OrthoDBEOG708W9T.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_ASPNC
AccessionPrimary (citable) accession number: A2Q9E4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 6, 2007
Last modified: June 11, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways