Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trm8

Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei101S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei191S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei194UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trm8
ORF Names:An01g07580
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006706 Componenti: Chromosome 2R

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003705871 – 297tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST297

Proteomic databases

PaxDbiA2Q9E4

Interactioni

Subunit structurei

Forms a complex with trm82.UniRule annotation

Protein-protein interaction databases

STRINGi5061.CADANGAP00000734

Structurei

3D structure databases

ProteinModelPortaliA2Q9E4
SMRiA2Q9E4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 125S-adenosyl-L-methionine bindingUniRule annotation2
Regioni171 – 172S-adenosyl-L-methionine bindingUniRule annotation2
Regioni270 – 272S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000260965
OrthoDBiEOG092C3FCG

Family and domain databases

HAMAPiMF_03055 tRNA_methyltr_TrmB_euk, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR025763 Trm8_euk
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
PTHR23417:SF16 PTHR23417:SF16, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

A2Q9E4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQPPAKRQK RAEYRKQAAE AVVAQPDESA PVAHVKLPKK KFYRQRAHAN
60 70 80 90 100
PFSDHQLNYP LSPAHMDWAS HFPAFVNPDA TQTNLIGTRK LLKDVEVVDI
110 120 130 140 150
GCGFGGLLVG LAGLLPETLM VGMEIRIAVL EYLNTRIQAL RVQQQQQQQK
160 170 180 190 200
TQSHAALVPG GYQNISAIRS NTMKFFPNFF NKHQLSKIFI CFPDPHFKAR
210 220 230 240 250
KHKARIISET LNAEYAYALR PGGLLYTITD VEEYHHWILR HFRDESALFE
260 270 280 290
RVSEEELEKD ECVKVMKEAT EEGKKVTRNK GNKYVAVFRR KEDPEWA
Length:297
Mass (Da):34,052
Last modified:March 6, 2007 - v1
Checksum:i84C5A7C435920A92
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM269973 Genomic DNA Translation: CAK96183.1

Genome annotation databases

EnsemblFungiiCAK96183; CAK96183; An01g07580

Similar proteinsi

Entry informationi

Entry nameiTRMB_ASPNC
AccessioniPrimary (citable) accession number: A2Q9E4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 6, 2007
Last modified: May 23, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health