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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trm8

Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei101S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei191S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei194UniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trm8
ORF Names:An01g07580
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006706 Componenti: Chromosome 2R

Subcellular locationi

  • Nucleus UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003705871 – 297tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST297

Interactioni

Subunit structurei

Forms a complex with trm82.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA2Q9E4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 125S-adenosyl-L-methionine bindingUniRule annotation2
Regioni171 – 172S-adenosyl-L-methionine bindingUniRule annotation2
Regioni270 – 272S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000260965.
OrthoDBiEOG092C3FCG.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2Q9E4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQPPAKRQK RAEYRKQAAE AVVAQPDESA PVAHVKLPKK KFYRQRAHAN
60 70 80 90 100
PFSDHQLNYP LSPAHMDWAS HFPAFVNPDA TQTNLIGTRK LLKDVEVVDI
110 120 130 140 150
GCGFGGLLVG LAGLLPETLM VGMEIRIAVL EYLNTRIQAL RVQQQQQQQK
160 170 180 190 200
TQSHAALVPG GYQNISAIRS NTMKFFPNFF NKHQLSKIFI CFPDPHFKAR
210 220 230 240 250
KHKARIISET LNAEYAYALR PGGLLYTITD VEEYHHWILR HFRDESALFE
260 270 280 290
RVSEEELEKD ECVKVMKEAT EEGKKVTRNK GNKYVAVFRR KEDPEWA
Length:297
Mass (Da):34,052
Last modified:March 6, 2007 - v1
Checksum:i84C5A7C435920A92
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM269973 Genomic DNA. Translation: CAK96183.1.

Genome annotation databases

EnsemblFungiiCAK96183; CAK96183; An01g07580.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM269973 Genomic DNA. Translation: CAK96183.1.

3D structure databases

ProteinModelPortaliA2Q9E4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAK96183; CAK96183; An01g07580.

Phylogenomic databases

HOGENOMiHOG000260965.
OrthoDBiEOG092C3FCG.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_ASPNC
AccessioniPrimary (citable) accession number: A2Q9E4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 6, 2007
Last modified: November 30, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.