A2Q7T1 (KATG_ASPNC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
ORF Names:	An01g01830

Organism

Aspergillus niger (strain CBS 513.88 / FGSC A1513) [Complete proteome]

Taxonomic identifier

425011 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	762 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. 2 H2O2 = O2 + 2 H2O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 762	762	Catalase-peroxidase		PRO_0000354101
Sites
	Active site	97	1	Proton acceptor By similarity
	Metal binding	283	1	Iron (heme axial ligand) By similarity
	Site	93	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	96 ↔ 242		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-268) By similarity
	Cross-link	242 ↔ 268		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-96) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2Q7T1 [UniParc]. Last modified March 6, 2007. Version 1. Checksum: 373EFFF72FC82CDC	FASTA	762	84,251
	10 20 30 40 50 60 MAEAKCPFSQ SRSNANVAGG GTRNTDWWPD QLNLGILRQH APASNPFERD FDYTAAFNSL 70 80 90 100 110 120 DYYALKKDLH ALMTDSQDWW PADFGHYGGL FIRMAWHSAG TYRVFDGRGG GGQGQQRFAP 130 140 150 160 170 180 LNSWPDNASL DKARRLLWPI KQKYGAKISW ADLMLLAGNV ALESMGFKTY GFSGGRADTW 190 200 210 220 230 240 EADESVYWGG ESTWMGNDVR YSDGFPGVTK HGALSGDEPP HRNIHTRDLE KPLAASHMGL 250 260 270 280 290 300 IYVNPEGPDG NPDPVAAARD IRTTFGRMGM NDEETVALIA GGHSFGKTHG AASSENVDVE 310 320 330 340 350 360 PAAAGLENQG LGWSNRYQSG KGPHTITSGI EVTWTKTPTK WSHAFLEYLF RFDWELTKSP 370 380 390 400 410 420 GGANQWQAKN TEAIIPDAYD PSKKHLPKML TTDLSLRYDP AYEKIARRFL DHPDEFADAF 430 440 450 460 470 480 SRAWFKLLHR DMGPRTRYIG PEAPTEDLIW QDPIPAVNHT LVDANDIAAL KRTILDTGLN 490 500 510 520 530 540 KSNFVSTAWA SASTFRGTDK RGGANGARIR LAPQRQWEVN NQPWLEETLS ALEKIQKDFN 550 560 570 580 590 600 DRVSSTGKKI SLADLIVLAG CAAVEKAAQE AGQTITVPFT PGRMDASQEQ TEVESFSHLE 610 620 630 640 650 660 PVADGFRNYG KSSSRVRAEH YLVDKAHLLT LTAPEMTVLV GGLRVLNTNY DGSKHGVLTS 670 680 690 700 710 720 SPGRLTNDFF TNVLDMNTAW KAKDGGRDLY EGTDRKTGQP KWTATRADLV FGSHAELRAL 730 740 750 760 AEVYGSSDGQ EKFVKDFVSA WDKVMNLDRF DLKGSGIARS KL

References

[1]

"Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88." Pel H.J., de Winde J.H., Archer D.B., Dyer P.S., Hofmann G., Schaap P.J., Turner G., de Vries R.P., Albang R., Albermann K., Andersen M.R., Bendtsen J.D., Benen J.A.E., van den Berg M., Breestraat S., Caddick M.X., Contreras R., Cornell M. , Coutinho P.M., Danchin E.G.J., Debets A.J.M., Dekker P., van Dijck P.W.M., van Dijk A., Dijkhuizen L., Driessen A.J.M., d'Enfert C., Geysens S., Goosen C., Groot G.S.P., de Groot P.W.J., Guillemette T., Henrissat B., Herweijer M., van den Hombergh J.P.T.W., van den Hondel C.A.M.J.J., van der Heijden R.T.J.M., van der Kaaij R.M., Klis F.M., Kools H.J., Kubicek C.P., van Kuyk P.A., Lauber J., Lu X., van der Maarel M.J.E.C., Meulenberg R., Menke H., Mortimer M.A., Nielsen J., Oliver S.G., Olsthoorn M., Pal K., van Peij N.N.M.E., Ram A.F.J., Rinas U., Roubos J.A., Sagt C.M.J., Schmoll M., Sun J., Ussery D., Varga J., Vervecken W., van de Vondervoort P.J.J., Wedler H., Woesten H.A.B., Zeng A.-P., van Ooyen A.J.J., Visser J., Stam H. Nat. Biotechnol. 25:221-231(2007) [PubMed: 17259976] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CBS 513.88 / FGSC A1513.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM269954 Genomic DNA. Translation: CAK43554.1.
RefSeq	XP_001388622.1. XM_001388585.2.
3D structure databases
ProteinModelPortal	A2Q7T1.
SMR	A2Q7T1. Positions 21-752.
ModBase	Search...
Protein family/group databases
PeroxiBase	5224. AnCP01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADANGAT00000180; CADANGAP00000171; CADANGAG00000180.
GeneID	4977099.
GenomeReviews	Gene locus katG in contig AM270980_GR.
KEGG	ang:ANI_1_228014.
Phylogenomic databases
GeneTree	EFGT00050000003307.
HOGENOM	HBG285610.
OrthoDB	EOG41CB4B.
Family and domain databases
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_ASPNC

Accession

Primary (citable) accession number: A2Q7T1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	March 6, 2007
Last modified:	November 16, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program