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Protein

Putative glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

YMR084W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins).By similarity

Caution

This is a truncated version of a putative glutamine--fructose-6-phosphate aminotransferase. Strain S288c has a frameshift in position 258, which disrupts the gene coding for this protein and produces two ORFs YMR084W and YMR085W. A contiguous sequence for this protein can be found in strain YJM789 (AC A6ZME2).Curated

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Putative glutamine--fructose-6-phosphate aminotransferase [isomerizing] (YMR084W), Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (GFA1)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Nucleophile; for GATase activityPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-32784-MONOMER
UniPathwayiUPA00113; UER00528

Names & Taxonomyi

Protein namesi
Recommended name:
Putative glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Short name:
GFAT
Alternative name(s):
D-fructose-6-phosphate amidotransferase
Hexosephosphate aminotransferase
Gene namesi
Ordered Locus Names:YMR084W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR084W
SGDiS000004689 YMR084W

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002697571 – 262Putative glutamine--fructose-6-phosphate aminotransferase [isomerizing]Add BLAST262

Proteomic databases

PaxDbiA2P2R3
PRIDEiA2P2R3

Interactioni

Protein-protein interaction databases

BioGridi35259, 78 interactors
STRINGi4932.YMR084W

Structurei

3D structure databases

ProteinModelPortaliA2P2R3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 262Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST261

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

HOGENOMiHOG000203549
InParanoidiA2P2R3
KOiK00820
OrthoDBiEOG092C0ST1

Family and domain databases

Gene3Di3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR017932 GATase_2_dom
IPR029055 Ntn_hydrolases_N
SUPFAMiSSF56235 SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit

Sequencei

Sequence statusi: Complete.

A2P2R3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIFGYCNF LIEKTRGEII DTLIEGLQAL EYKEYDSSGI SIQGDELESL
60 70 80 90 100
NIYKQTGKIS SLKEEIDLYN LNKNLPFISH CGIAHTRRAT HGGLRRANCH
110 120 130 140 150
PHNSDPSNEF VVVHNGVITN FANLKALLMA KGYVFKSDTD TECIPKLYKH
160 170 180 190 200
IYDTSIELGY NLDFHVLTNL VLKELEGSYG LLCTSSHFPD EVVAARKGSP
210 220 230 240 250
LVIGVKGKTD MDVNFVEVEY LDQEEDYLKL NTQTKSSGNV LAAAPVKYNT
260
CLRKSPPFVH NT
Length:262
Mass (Da):29,272
Last modified:February 20, 2007 - v1
Checksum:iCAEC5457BEB33DFD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49259 Genomic DNA Translation: CAA89230.1
AY692572 Genomic DNA Translation: AAT92591.1
AY268137 Genomic DNA Translation: AAR26283.1
BK006946 Genomic DNA Translation: DAA09981.1
PIRiS54459
RefSeqiNP_013801.1, NM_001182583.1

Genome annotation databases

EnsemblFungiiYMR084W; YMR084W; YMR084W
GeneIDi855108
KEGGisce:YMR084W

Similar proteinsi

Entry informationi

Entry nameiYM084_YEAST
AccessioniPrimary (citable) accession number: A2P2R3
Secondary accession number(s): D6VZQ7
, E9P8R5, Q3E7C0, Q6B308, Q6WV01
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: February 20, 2007
Last modified: April 25, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health