A2KF29 (SMKTR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sperm motility kinase Tcr mutant form
Short name=SmokTcr
Short name=Tcr
EC=2.7.11.1

Alternative name(s):
Dominant negative form of Smok
Responder protein Smok-Tcr

Gene names

Name:

Smoktcr

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	While the main function of Smoks is to control sperm motility, the role of Smok-Tcr, with reduced kinase activity, is to counterbalance a signaling impairment caused by the distorter/sterility loci, giving t-sperm an advantage in reaching the oocytes. Transmission ratio distortion also called segregation distortion is the name given to the phenomenon above-mentioned. Being associated with the T-complex, it allows males heterozygous for a complete t-haplotype to preferentially transmit the t-haplotype chromosome. Ref.1
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Tissue specificity	Testis-specific. Expressed in the testis from 22 days postpartum (22 dpp). Expressed late in spermiogenesis, only in Tcr-containing t-haplotypes. Ref.1
Miscellaneous	Encoded on the T-complex, a region of 20-30 Mb on proximal third of mouse chromosome 17. Naturally occurring variant forms of the T-complex, known as complete t-haplotypes, are found in wild mouse populations. The t-haplotypes contain at least four nonoverlapping inversions that suppress recombination with the wild-type chromosome, and lock into strong linkage disequilibrium loci affecting normal transmission of the chromosome, male gametogenesis and embryonic development. T6 allele.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Smok subfamily. Contains 1 protein kinase domain.
Caution	Although it is derived from a rearrangement with the neighboring RSK3 gene, it plays a crucial role in t-haplotype cells.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	Sperm motility kinase Tcr mutant form		PRO_0000307873
Regions
	Domain	8 – 256	249	Protein kinase
	Nucleotide binding	14 – 22	9	ATP By similarity
Sites
	Active site	127	1	Proton acceptor By similarity
	Binding site	37	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2KF29 [UniParc]. Last modified February 20, 2007. Version 1. Checksum: A84F3BDCE420C973	FASTA	484	54,619
	10 20 30 40 50 60 MEKFHAQYEM LETIGQGGCA QVKLARHRLT GTHVAVKVIV KRECWFNPVM SEAELLMMTD 70 80 90 100 110 120 HPNIISLLQV IETKKKVYLI MELCEGKSLY QHIQNAGYLQ EDEARPLFKQ LLSAMNYCHN 130 140 150 160 170 180 QGIVHRDLTP DNIMVEKDGK VKIIDFGLGT QEKPGQNHNL FCEIYPFSTP EVLFNRPYDM 190 200 210 220 230 240 RKIDVWGLGV VLYFMVTGKI LFDTASVEKL RKQIVAEKCS VPCRLSVELQ DLIRLLMTDI 250 260 270 280 290 300 PELRPTVAEV MVHPWVTEGS GVLPDPCEEH IPLKPDPAIA KAMGFIGFQA QDIEDSLCQR 310 320 330 340 350 360 KFNETMASYC LLKKQILKEC DRPIRAQPMN PSVTPLSSLV DAPTFHLGLR RTETEPTGLR 370 380 390 400 410 420 LSDNKEVPVC GNSTSKKRER SFSGPGVLSR PINTTPTMDQ THTRTWSGPC IYSNVCTIHP 430 440 450 460 470 480 NSINESTEGH ISTSAEDKPV HSRGWPRGIK GWTRKIGNAM RKLCCCIPSK ETSHLGQRRV CPKI

References

[1]

"A protein kinase encoded by the t complex responder gene causes non-Mendelian inheritance." Herrmann B.G., Koschorz B., Wertz K., McLaughlin K.J., Kispert A. Nature 402:141-146(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, FUNCTION. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ245452 mRNA. Translation: CAB61340.1.
IPI	IPI00828473.
3D structure databases
ProteinModelPortal	A2KF29.
SMR	A2KF29. Positions 4-322.
ModBase	Search...
Proteomic databases
PRIDE	A2KF29.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG108494.
Gene expression databases
Genevestigator	A2KF29.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008266. Tyr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	35544435.

Entry information

Entry name

SMKTR_MOUSE

Accession

Primary (citable) accession number: A2KF29

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	February 20, 2007
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program