Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A2KF29 (SMKTR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sperm motility kinase Tcr mutant form

Short name=SmokTcr
Short name=Tcr
EC=2.7.11.1
Alternative name(s):
Dominant negative form of Smok
Responder protein Smok-Tcr
Gene names
Name:Smoktcr
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length484 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

While the main function of Smoks is to control sperm motility, the role of Smok-Tcr, with reduced kinase activity, is to counterbalance a signaling impairment caused by the distorter/sterility loci, giving t-sperm an advantage in reaching the oocytes. Transmission ratio distortion also called segregation distortion is the name given to the phenomenon above-mentioned. Being associated with the T-complex, it allows males heterozygous for a complete t-haplotype to preferentially transmit the t-haplotype chromosome. Ref.1

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Tissue specificity

Testis-specific. Expressed in the testis from 22 days postpartum (22 dpp). Expressed late in spermiogenesis, only in Tcr-containing t-haplotypes. Ref.1

Miscellaneous

Encoded on the T-complex, a region of 20-30 Mb on proximal third of mouse chromosome 17. Naturally occurring variant forms of the T-complex, known as complete t-haplotypes, are found in wild mouse populations. The t-haplotypes contain at least four nonoverlapping inversions that suppress recombination with the wild-type chromosome, and lock into strong linkage disequilibrium loci affecting normal transmission of the chromosome, male gametogenesis and embryonic development.

T6 allele.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Smok subfamily.

Contains 1 protein kinase domain.

Caution

Although it is derived from a rearrangement with the neighboring RSK3 gene, it plays a crucial role in t-haplotype cells.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 484484Sperm motility kinase Tcr mutant form
PRO_0000307873

Regions

Domain8 – 256249Protein kinase
Nucleotide binding14 – 229ATP By similarity

Sites

Active site1271Proton acceptor By similarity
Binding site371ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A2KF29 [UniParc].

Last modified February 20, 2007. Version 1.
Checksum: A84F3BDCE420C973

FASTA48454,619
        10         20         30         40         50         60 
MEKFHAQYEM LETIGQGGCA QVKLARHRLT GTHVAVKVIV KRECWFNPVM SEAELLMMTD 

        70         80         90        100        110        120 
HPNIISLLQV IETKKKVYLI MELCEGKSLY QHIQNAGYLQ EDEARPLFKQ LLSAMNYCHN 

       130        140        150        160        170        180 
QGIVHRDLTP DNIMVEKDGK VKIIDFGLGT QEKPGQNHNL FCEIYPFSTP EVLFNRPYDM 

       190        200        210        220        230        240 
RKIDVWGLGV VLYFMVTGKI LFDTASVEKL RKQIVAEKCS VPCRLSVELQ DLIRLLMTDI 

       250        260        270        280        290        300 
PELRPTVAEV MVHPWVTEGS GVLPDPCEEH IPLKPDPAIA KAMGFIGFQA QDIEDSLCQR 

       310        320        330        340        350        360 
KFNETMASYC LLKKQILKEC DRPIRAQPMN PSVTPLSSLV DAPTFHLGLR RTETEPTGLR 

       370        380        390        400        410        420 
LSDNKEVPVC GNSTSKKRER SFSGPGVLSR PINTTPTMDQ THTRTWSGPC IYSNVCTIHP 

       430        440        450        460        470        480 
NSINESTEGH ISTSAEDKPV HSRGWPRGIK GWTRKIGNAM RKLCCCIPSK ETSHLGQRRV 


CPKI 

« Hide

References

[1]"A protein kinase encoded by the t complex responder gene causes non-Mendelian inheritance."
Herrmann B.G., Koschorz B., Wertz K., McLaughlin K.J., Kispert A.
Nature 402:141-146(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, FUNCTION.
Tissue: Testis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ245452 mRNA. Translation: CAB61340.1.

3D structure databases

ProteinModelPortalA2KF29.
SMRA2KF29. Positions 5-313.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEA2KF29.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG108494.

Gene expression databases

GenevestigatorA2KF29.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio35544435.
PROA2KF29.

Entry information

Entry nameSMKTR_MOUSE
AccessionPrimary (citable) accession number: A2KF29
Entry history
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: February 20, 2007
Last modified: April 16, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index