ID   A2GAK8_TRIV3            Unreviewed;       607 AA.
AC   A2GAK8;
DT   20-FEB-2007, integrated into UniProtKB/TrEMBL.
DT   20-FEB-2007, sequence version 1.
DT   27-MAR-2024, entry version 82.
DE   RecName: Full=Alpha-amylase {ECO:0000256|ARBA:ARBA00012595, ECO:0000256|RuleBase:RU361134};
DE            EC=3.2.1.1 {ECO:0000256|ARBA:ARBA00012595, ECO:0000256|RuleBase:RU361134};
GN   ORFNames=TVAG_537410 {ECO:0000313|EMBL:EAX85812.1};
OS   Trichomonas vaginalis (strain ATCC PRA-98 / G3).
OC   Eukaryota; Metamonada; Parabasalia; Trichomonadida; Trichomonadidae;
OC   Trichomonas.
OX   NCBI_TaxID=412133 {ECO:0000313|EMBL:EAX85812.1, ECO:0000313|Proteomes:UP000001542};
RN   [1] {ECO:0000313|EMBL:EAX85812.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=G3 {ECO:0000313|EMBL:EAX85812.1};
RA   Amadeo P., Zhao Q., Wortman J., Fraser-Liggett C., Carlton J.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:EAX85812.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=G3 {ECO:0000313|EMBL:EAX85812.1};
RX   PubMed=17218520; DOI=10.1126/science.1132894;
RA   Carlton J.M., Hirt R.P., Silva J.C., Delcher A.L., Schatz M., Zhao Q.,
RA   Wortman J.R., Bidwell S.L., Alsmark U.C.M., Besteiro S.,
RA   Sicheritz-Ponten T., Noel C.J., Dacks J.B., Foster P.G., Simillion C.,
RA   Van de Peer Y., Miranda-Saavedra D., Barton G.J., Westrop G.D., Mueller S.,
RA   Dessi D., Fiori P.L., Ren Q., Paulsen I., Zhang H., Bastida-Corcuera F.D.,
RA   Simoes-Barbosa A., Brown M.T., Hayes R.D., Mukherjee M., Okumura C.Y.,
RA   Schneider R., Smith A.J., Vanacova S., Villalvazo M., Haas B.J., Pertea M.,
RA   Feldblyum T.V., Utterback T.R., Shu C.L., Osoegawa K., de Jong P.J.,
RA   Hrdy I., Horvathova L., Zubacova Z., Dolezal P., Malik S.B.,
RA   Logsdon J.M. Jr., Henze K., Gupta A., Wang C.C., Dunne R.L., Upcroft J.A.,
RA   Upcroft P., White O., Salzberg S.L., Tang P., Chiu C.-H., Lee Y.-S.,
RA   Embley T.M., Coombs G.H., Mottram J.C., Tachezy J., Fraser-Liggett C.M.,
RA   Johnson P.J.;
RT   "Draft genome sequence of the sexually transmitted pathogen Trichomonas
RT   vaginalis.";
RL   Science 315:207-212(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in
CC         polysaccharides containing three or more (1->4)-alpha-linked D-
CC         glucose units.; EC=3.2.1.1; Evidence={ECO:0000256|ARBA:ARBA00000548,
CC         ECO:0000256|RuleBase:RU361134};
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|ARBA:ARBA00001913};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC       {ECO:0000256|ARBA:ARBA00008061, ECO:0000256|RuleBase:RU003615}.
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DR   EMBL; DS114826; EAX85812.1; -; Genomic_DNA.
DR   RefSeq; XP_001298742.1; XM_001298741.1.
DR   AlphaFoldDB; A2GAK8; -.
DR   STRING; 5722.A2GAK8; -.
DR   GeneID; 4743455; -.
DR   KEGG; tva:TVAG_2v0790270; -.
DR   VEuPathDB; TrichDB:TVAG_537410; -.
DR   VEuPathDB; TrichDB:TVAGG3_0790270; -.
DR   eggNOG; KOG2212; Eukaryota.
DR   InParanoid; A2GAK8; -.
DR   OMA; RTHRDIQ; -.
DR   Proteomes; UP000001542; Unassembled WGS sequence.
DR   GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0043169; F:cation binding; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd11317; AmyAc_bac_euk_AmyA; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR006046; Alpha_amylase.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR   PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   PRINTS; PR00110; ALPHAAMYLASE.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism {ECO:0000256|RuleBase:RU361134};
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361134};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361134};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001542}.
FT   DOMAIN          161..605
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
FT   REGION          463..493
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        471..488
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   607 AA;  67968 MW;  50CCA42F30793DE2 CRC64;
     MLISFAVNGR FGAANQYDPL PNCNIHPQMS CAGSSGDIDP KWFGNLWNTP KRGKENWKPG
     FQDMSDLTGY AQLKYASGRQ SCTVNIITKT SKDLDLTFYF DGVAQKTNSK TFDSSFNKLL
     KVKVVAATGE SLILDDIDFV RNVAPLGQRS NDQRYRNGQK GAIIELFGWP DADIEQECKF
     IADAGYLGVK VFPHQEQVMS SQTFEKELNP WYFMYQPVSY RLQGRMGTRD QLRNMINTCR
     RYGVRVYADA VVNHMTGNGN DLSNHRNPSA GCTKWGNKTS SAYELGSPYY TPAYTYETNP
     NTGRGTNVLE FPAVPYGPED FHCDKALGSW SDPNILNTGW LSGLSDLDTS KDYVRQRIAD
     FMIDLISIGF SGYRIDAAKH IHPKDLAAIF GKVKQGLGGS LPDDFISWLE VLTGGEAYLL
     VQGDGDYSFT GGLTKFLKQN GLNDDEILKI KIWWCGYPTE PGNDNGSIDP HRKVIQNDDH
     DQQNDGSSSR DMHDAGCVLV KGCDPAKHRE YEKRLFKNPA GFESNNKDAA PIRLVLSSYY
     WGDNNVHSIP DGESDCKKCT LSCESCHTRE YIKAYNPSAT SYPGGKGYTY VHRDSEIIQE
     MNNWMNL
//