A2CI41 (FTSI_CHLAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptidoglycan synthase ftsI homolog
EC=2.4.1.129

Alternative name(s):
Peptidoglycan glycosyltransferase

Gene names

Name:

ftsI

Encoded on

Plastid; Chloroplast

Organism

Chlorokybus atmophyticus (Soil alga)

Taxonomic identifier

3144 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Chlorokybophyceae › Chlorokybales › Chlorokybaceae › Chlorokybus

Protein attributes

Sequence length	679 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Essential for the formation of a septum of the murein sacculus. Synthesis of cross-linked peptidoglycan from the lipid intermediates Potential.
Catalytic activity	(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	Plastid › chloroplast membrane; Single-pass membrane protein Potential.
Miscellaneous	The presence of this gene in the chloroplast genome suggests there may be an unsuspected vestigal peptidoglycan layer in this organism's chloroplasts.
Sequence similarities	Belongs to the transpeptidase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Peptidoglycan synthesis
Cellular component	Chloroplast Membrane Plastid
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	penicillin binding Inferred from electronic annotation. Source: InterPro peptidoglycan glycosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 679	679	Peptidoglycan synthase ftsI homolog		PRO_0000314459
Regions
	Transmembrane	13 – 33	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	A2CI41 [UniParc]. Last modified February 20, 2007. Version 1. Checksum: 55BEB81500C9F33E	FASTA	679	75,878
	10 20 30 40 50 60 MKPYEPKSWV TRVFLVWWLT ALSCFFISGR LIYLQLLKGK WLKEKALKQQ TVTLKTFQPR 70 80 90 100 110 120 RNICDRNGIP LAIDTLAYDV FAHPLYFSIS IEEVANKLSP ILCIDSLSIQ KLLKPTSTGI 130 140 150 160 170 180 CLASQLPENT GKLIASLRLD GIDLIKHPKR YYPYKEIVGN VIGYVDTSHQ GQAGIELSCQ 190 200 210 220 230 240 ESLQLNSPTL TSSIDGRGVL ISHQIPKELF IQDNLSLQLT LDLELQKIAY KALKQGLENC 250 260 270 280 290 300 KGKRGTVLIL DPKTGGILTL VALPSYDPNI YYDFPIERFK PWPVTDLYEP GSTFKPLNIA 310 320 330 340 350 360 IALETKAISP EDSFYDEGCI RVGDSIITNN DYNSYKPLPC LPNTYNKIVK LLANSSNVGM 370 380 390 400 410 420 VHILERIAPE IYHSWLSKLD LGHAASPLET DLPWASESSL KDINEFVCYE IEPAAASFGQ 430 440 450 460 470 480 GLAMTPIKLA QLYASLANGG ILVKPYLVTG LANAAEDTQK AKGIDLPSYN IRKKNLGNHL 490 500 510 520 530 540 SWHKAEPSYL FLKRSGIRVT DLLRHIKAEG RFALPFRKNL LQLFTQDAHR TTELQLEPKA 550 560 570 580 590 600 HQPQLLRPTR HAVYATNQSK RVFSHETTKL LLDMLEDVIW NGTGSSCFVE GYRIGGKTGT 610 620 630 640 650 660 SQKHTQEGGY SKTKIITSFA AIFPTEDPQY VILTVIDEPN IPLSFGSNTA APIVKSIIES 670 LIDIKKMKPT IPIIKVKKD

References

[1]

"A clade uniting the green algae Mesostigma viride and Chlorokybus atmophyticus represents the deepest branch of the Streptophyta in chloroplast genome-based phylogenies." Lemieux C., Otis C., Turmel M. BMC Biol. 5:2-2(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SAG 48.80.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ422812 Genomic DNA. Translation: ABM87954.1.
RefSeq	YP_001019071.1. NC_008822.1.
3D structure databases
ProteinModelPortal	A2CI41.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4783320.
Phylogenomic databases
ProtClustDB	CLSC858421.
Enzyme and pathway databases
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.40.710.10. 2 hits.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR005311. PBP_dimer. IPR001460. PCN-bd_Tpept. [Graphical view]
Pfam	PF03717. PBP_dimer. 1 hit. PF00905. Transpeptidase. 2 hits. [Graphical view]
SUPFAM	SSF56519. PBP_dimer. 1 hit. SSF56601. PBP_transp_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FTSI_CHLAT

Accession

Primary (citable) accession number: A2CI41

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 20, 2007
Last modified:	March 6, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program