Reviewed, UniProtKB/Swiss-Prot A2CI34 (DUSTY_PIMPR)

Names and origin

Protein names

Recommended name:
    Dual serine/threonine and tyrosine protein kinase
    EC=2.7.12.1
Alternative name(s):
    Dusty protein kinase
      Short name=Dusty PK
    Receptor-interacting serine/threonine-protein kinase 5

Gene names

Name:	dstyk
Synonyms:	ripk5

Organism

Pimephales promelas (Fathead minnow)

Taxonomic identifier

90988 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Pimephales

Protein attributes

Sequence length	903 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May induce both caspase-dependent apoptosis and caspase-independent cell death By similarity. UniProtKB Q6XUX3
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. UniProtKB P16879
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 903	903	Dual serine/threonine and tyrosine protein kinase		PRO_0000374057
Regions
	Domain	627 – 881	255	Protein kinase
	Nucleotide binding	633 – 641	9	ATP By similarity
	Coiled coil	382 – 414	33	Potential
Sites
	Active site	752	1	Proton acceptor By similarity
	Binding site	656	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2CI34-1 [UniParc]. Last modified February 20, 2007. Version 1. Checksum: 82D50FDA42F415C6	FASTA	903	101,593
	10 20 30 40 50 60 MENPQKAGPL LRDLTRAFSH YNKHNLLLKK NLKETIAFFR EIRQNHSNTC STSGPELDSG 70 80 90 100 110 120 QLRCISFPRH DEDHLQKVVG CAPYILILGQ DCSARYQLLN CMLGERLLPL GSDAGGACGV 130 140 150 160 170 180 EGGACRRRKL CFTHGRQTRL SLALPGQYEL VHQLAAHCGR WDTVPREDLE IQECEDPAQR 190 200 210 220 230 240 LAELEITLHH ALLQEAKIMV LPCRNVQPVE EALEDCRRGI LPIILYAVSK ATLSADQLSE 250 260 270 280 290 300 LQKVRETLPY PVCFVRIPTE PAPDPPGQRS ALFAQLVSQE LIGGAAGNCA CGAPAQTPGK 310 320 330 340 350 360 MQGILGEDLE RLHRVLVPFA RQVLQSQQVE ATTLLNAVHC RCLDLFINQA FDMQRDLQIT 370 380 390 400 410 420 PRRLEYTREK EGELYSSLMA IANRKQEEMK EMIVETLESM KEQLLEDAAN LEFTDIIMTS 430 440 450 460 470 480 NSEPMSSKDI KVCISQIQDL IVIRLNQAVA NKLTSSVDYL RESFVGTLER CLCSLEKSTG 490 500 510 520 530 540 EPCAHNVTSN HLKQILNAAY HVEVTFHSGS SVTRLFWEQI KQIIHRISWV NPPSVTSEWK 550 560 570 580 590 600 RKVAQDAIES LSAAKLAKSI CSQFRTRLNS SHEAFASSLR QLEEGHTGRL ERTEDLWLRV 610 620 630 640 650 660 RKDHAPRLAR LSLESRSLRD ILLHGKPKLG RELGRGQYGV VYLCDNWAGR HPCALKSVVP 670 680 690 700 710 720 PDDKHWNDLA LEFHYTRSLP KHERLVNLHG SVIDHSYGGG SSIAVLLIME RLHRDLYTGL 730 740 750 760 770 780 KAGLVLKERL QIALDVVEGI RFLHGQGLLH RDIKLKNVLL DKQNRAKITD LGFCKPEAMM 790 800 810 820 830 840 SGSIVGTPIH MAPELFTGKY DNSVDVYAFG ILFWYLCTGS VKLPEAFERC SSKDQLWTNV 850 860 870 880 890 900 KKGSRPERLA SFDEECWQLM EACWNGDPSQ RPLLGIVQPS LQSIMDRLCN DSDQKSGNLE DSN

References

[1]

"Dusty protein kinases: primary structure, gene evolution, tissue specific expression and unique features of the catalytic domain." Peng J., Dong W., Chen Y., Mo R., Cheng J.-F., Hui C.-C., Mohandas N., Huang C.-H. Biochim. Biophys. Acta 1759:562-572(2006) [PubMed: 17123648] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ419945 mRNA. Translation: ABD77593.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	A2CI34.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DUSTY_PIMPR

Accession

Primary (citable) accession number: A2CI34

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	February 20, 2007
Last modified:	October 13, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)