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Protein

AT-rich interactive domain-containing protein 4B

Gene

Arid4b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • establishment of Sertoli cell barrier Source: MGI
  • histone H3-K9 trimethylation Source: MGI
  • histone H4-K20 trimethylation Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of gene expression by genetic imprinting Source: MGI
  • spermatogenesis Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
AT-rich interactive domain-containing protein 4B
Short name:
ARID domain-containing protein 4B
Alternative name(s):
180 kDa Sin3-associated polypeptide
Short name:
Sin3-associated polypeptide p180
Histone deacetylase complex subunit SAP180
Gene namesi
Name:Arid4b
Synonyms:Sap180
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2137512. Arid4b.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002828641 – 1314AT-rich interactive domain-containing protein 4BAdd BLAST1314

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei276PhosphoserineBy similarity1
Modified residuei295PhosphoserineBy similarity1
Modified residuei296PhosphoserineBy similarity1
Cross-linki428Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei482PhosphoserineBy similarity1
Modified residuei666PhosphoserineBy similarity1
Modified residuei668PhosphoserineCombined sources1
Modified residuei675PhosphoserineCombined sources1
Modified residuei717PhosphoserineBy similarity1
Modified residuei778PhosphoserineBy similarity1
Modified residuei790PhosphoserineCombined sources1
Modified residuei793PhosphothreonineCombined sources1
Modified residuei1016PhosphoserineBy similarity1
Modified residuei1028PhosphothreonineBy similarity1
Modified residuei1031PhosphoserineBy similarity1
Modified residuei1152PhosphothreonineBy similarity1
Modified residuei1154PhosphoserineBy similarity1
Modified residuei1155PhosphoserineBy similarity1
Modified residuei1157PhosphoserineBy similarity1
Modified residuei1161PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiA2CG63.
MaxQBiA2CG63.
PaxDbiA2CG63.
PeptideAtlasiA2CG63.
PRIDEiA2CG63.

PTM databases

iPTMnetiA2CG63.
PhosphoSitePlusiA2CG63.

Expressioni

Gene expression databases

BgeeiENSMUSG00000039219.
CleanExiMM_ARID4B.
ExpressionAtlasiA2CG63. baseline and differential.
GenevisibleiA2CG63. MM.

Interactioni

Subunit structurei

Component of a Sin3A corepressor complex consisting of SIN3A, SAP130, SUDS3/SAP45, SAP180, HDAC1 and HDAC2.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Stra8P702784EBI-6394082,EBI-6394115

Protein-protein interaction databases

BioGridi220492. 2 interactors.
IntActiA2CG63. 1 interactor.
STRINGi10090.ENSMUSP00000106163.

Structurei

3D structure databases

ProteinModelPortaliA2CG63.
SMRiA2CG63.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini306 – 398ARIDPROSITE-ProRule annotationAdd BLAST93

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1227 – 1272Sequence analysisAdd BLAST46

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi154 – 159Poly-Ser6
Compositional biasi268 – 567Glu-richAdd BLAST300
Compositional biasi1097 – 1103Poly-Ser7
Compositional biasi1273 – 1305Ser-richAdd BLAST33

Domaini

The C-terminus mediates interaction with mSin3A corepressor complex.By similarity
The N-terminus is involved in transcriptional repression by HDAC-independent mechanisms.By similarity
The ARID domain is involved in stabilizing the mSin3A corepressor complex on DNA.By similarity

Sequence similaritiesi

Contains 1 ARID domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2744. Eukaryota.
KOG3001. Eukaryota.
ENOG410Y2AP. LUCA.
GeneTreeiENSGT00850000132287.
HOVERGENiHBG058247.
InParanoidiA2CG63.
KOiK19195.
OMAiPCKECEN.
OrthoDBiEOG091G0EG4.
PhylomeDBiA2CG63.
TreeFamiTF106427.

Family and domain databases

Gene3Di1.10.150.60. 1 hit.
InterProiIPR028853. ARID4B.
IPR001606. ARID_dom.
IPR016197. Chromodomain-like.
IPR012603. RBB1NT.
IPR002999. Tudor.
IPR025995. Tudor-knot.
[Graphical view]
PANTHERiPTHR13964:SF24. PTHR13964:SF24. 1 hit.
PfamiPF01388. ARID. 1 hit.
PF08169. RBB1NT. 1 hit.
PF11717. Tudor-knot. 1 hit.
[Graphical view]
SMARTiSM00501. BRIGHT. 1 hit.
SM00333. TUDOR. 2 hits.
[Graphical view]
SUPFAMiSSF46774. SSF46774. 1 hit.
SSF54160. SSF54160. 1 hit.
PROSITEiPS51011. ARID. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A2CG63-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKALDEPPYL TVGTDVSAKY RGAFCEAKIK TAKRLVKVKV TFRHDSSTVE
60 70 80 90 100
VQDDHIKGPL KVGAIVEVKN LDGAYQEAVI NKLTDASWYT VVFDDGDEKT
110 120 130 140 150
LRRSSLCLKG ERHFAESETL DQLPLTNPEH FGTPVIGKKT NRGRRSNHIP
160 170 180 190 200
EEESSSSSSD DDEEERKQTD ELLGKVVCVD YVSLEKKKAM WFPALVVCPD
210 220 230 240 250
CSDEIAVKKD NILVRSFKDG KFTSVPRKDV HEITSDTVPK PDAVLKQAFD
260 270 280 290 300
QALEFHKSRA IPANWKTELK EDSSSSEAEE EEEEEDDEKE KEDNSSEEEE
310 320 330 340 350
EIEPFPEERE NFLQQLYKFM EDRGTPINKR PVLGYRNLNL FKLFRLVHKL
360 370 380 390 400
GGFDNIESGA VWKQVYQDLG IPVLNSAAGY NVKCAYKKYL YGFEEYCRSA
410 420 430 440 450
NIDFQMALPE KVLNKPCKDC ENKEVKVKEE SETEIKEVNV EDSKNVMPKE
460 470 480 490 500
ETPAEDESER KENIKPSLGS KKSLLECIPA QSDEEKEAHI TKLEENENLE
510 520 530 540 550
DKDGGRARTE EAFSTEVDGE EEQARSGDET NKEEDEDDEE IEEEEEEDEE
560 570 580 590 600
EDEDEDDDDN NEEEEFECYP PGMKVQVRYG RGKNQKMYEA SIKDSDVEGG
610 620 630 640 650
EALYLVHYCG WNVRYDEWIK ADKIVRPADK NVPKIKHRKK IKNKLDKEKD
660 670 680 690 700
RDEKYSPKNC KLRRLSKSPF QSNPSPEMVS KLDLADAKNS DTAHIKSIEI
710 720 730 740 750
TSILNGLQAS ESSAEDSEQE DERCTQDVDN IGKDESKVEH STHSRNELIS
760 770 780 790 800
KEEQSSPSLL EENKVHTDLV IAKTVSKSPE RLRKDMEAIS EDTDFEEEDE
810 820 830 840 850
ITKKRKDVKK DTTDKALKPQ TKRGKRRYCS ADECLQTGSP GKKEDRTKSK
860 870 880 890 900
EPLCTENSSN SSSDEDEEEK SKAKMTPTKK YNGLEEKRKS LRTTSFYSGF
910 920 930 940 950
SEVAEKRIKL LNNSDERLQN NRAKDRKDVW SSIQGQWPKK TLKELFSDSD
960 970 980 990 1000
TEAAASPPHP APDEGAVEES LQTVAEEESC SPIMELEKPL PASVDNKPIE
1010 1020 1030 1040 1050
EKPLEVSDRK TEFPSSGSNS VLNTPPTTPE SPSSVTITEA SQQQSSVTVS
1060 1070 1080 1090 1100
VPLPPNQEEV RSIKSETDST IEVDSVVGEL QDLQSEGNSS PAGFDASVSS
1110 1120 1130 1140 1150
SSSNQPEPDN PEKACTGQKR VKDTQGVGSS SKKQKRSHKA TVVNNKKKGK
1160 1170 1180 1190 1200
GTNSSDSEEL SAGESVTKTQ TIKSVPTGMK THNSKSPARV QSPGKGGRNG
1210 1220 1230 1240 1250
DKDPDLKEPS NRLPKVYKWS FQTSDLENMT SAERISILQE KLQEIRKHYL
1260 1270 1280 1290 1300
SLKSEVASID RRRKRLKKKE RESAATSSSS SSPSSSSITA AVMLTLAEPS
1310
MSSASQNGMS VECR
Length:1,314
Mass (Da):147,643
Last modified:February 20, 2007 - v1
Checksum:iDE5E69DAA208A200
GO
Isoform 2 (identifier: A2CG63-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     528-614: Missing.

Show »
Length:1,227
Mass (Da):137,325
Checksum:i28B18F3DB2D6884D
GO

Sequence cautioni

The sequence CAM21242 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_024236528 – 614Missing in isoform 2. CuratedAdd BLAST87

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT025604 Genomic DNA. Translation: CAM21242.1. Sequence problems.
CT025604 Genomic DNA. Translation: CAM21243.1.
CT025604 Genomic DNA. Translation: CAM21244.1.
CT025604 Genomic DNA. Translation: CAM21245.1.
BC137783 mRNA. Translation: AAI37784.1.
AK031062 mRNA. Translation: BAC27233.1.
AK041414 mRNA. Translation: BAC30936.1.
CCDSiCCDS36599.1. [A2CG63-1]
CCDS36600.1. [A2CG63-2]
RefSeqiNP_919238.1. NM_194262.2. [A2CG63-1]
NP_937755.1. NM_198122.2. [A2CG63-2]
XP_006516869.1. XM_006516806.2. [A2CG63-1]
XP_006516870.1. XM_006516807.2. [A2CG63-1]
XP_017171137.1. XM_017315648.1. [A2CG63-1]
UniGeneiMm.439784.

Genome annotation databases

EnsembliENSMUST00000039538; ENSMUSP00000043889; ENSMUSG00000039219. [A2CG63-2]
ENSMUST00000110534; ENSMUSP00000106163; ENSMUSG00000039219. [A2CG63-1]
ENSMUST00000110536; ENSMUSP00000106165; ENSMUSG00000039219. [A2CG63-2]
GeneIDi94246.
KEGGimmu:94246.
UCSCiuc007pmz.2. mouse. [A2CG63-1]
uc007pna.2. mouse. [A2CG63-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT025604 Genomic DNA. Translation: CAM21242.1. Sequence problems.
CT025604 Genomic DNA. Translation: CAM21243.1.
CT025604 Genomic DNA. Translation: CAM21244.1.
CT025604 Genomic DNA. Translation: CAM21245.1.
BC137783 mRNA. Translation: AAI37784.1.
AK031062 mRNA. Translation: BAC27233.1.
AK041414 mRNA. Translation: BAC30936.1.
CCDSiCCDS36599.1. [A2CG63-1]
CCDS36600.1. [A2CG63-2]
RefSeqiNP_919238.1. NM_194262.2. [A2CG63-1]
NP_937755.1. NM_198122.2. [A2CG63-2]
XP_006516869.1. XM_006516806.2. [A2CG63-1]
XP_006516870.1. XM_006516807.2. [A2CG63-1]
XP_017171137.1. XM_017315648.1. [A2CG63-1]
UniGeneiMm.439784.

3D structure databases

ProteinModelPortaliA2CG63.
SMRiA2CG63.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi220492. 2 interactors.
IntActiA2CG63. 1 interactor.
STRINGi10090.ENSMUSP00000106163.

PTM databases

iPTMnetiA2CG63.
PhosphoSitePlusiA2CG63.

Proteomic databases

EPDiA2CG63.
MaxQBiA2CG63.
PaxDbiA2CG63.
PeptideAtlasiA2CG63.
PRIDEiA2CG63.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039538; ENSMUSP00000043889; ENSMUSG00000039219. [A2CG63-2]
ENSMUST00000110534; ENSMUSP00000106163; ENSMUSG00000039219. [A2CG63-1]
ENSMUST00000110536; ENSMUSP00000106165; ENSMUSG00000039219. [A2CG63-2]
GeneIDi94246.
KEGGimmu:94246.
UCSCiuc007pmz.2. mouse. [A2CG63-1]
uc007pna.2. mouse. [A2CG63-2]

Organism-specific databases

CTDi51742.
MGIiMGI:2137512. Arid4b.

Phylogenomic databases

eggNOGiKOG2744. Eukaryota.
KOG3001. Eukaryota.
ENOG410Y2AP. LUCA.
GeneTreeiENSGT00850000132287.
HOVERGENiHBG058247.
InParanoidiA2CG63.
KOiK19195.
OMAiPCKECEN.
OrthoDBiEOG091G0EG4.
PhylomeDBiA2CG63.
TreeFamiTF106427.

Miscellaneous databases

ChiTaRSiArid4b. mouse.
PROiA2CG63.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039219.
CleanExiMM_ARID4B.
ExpressionAtlasiA2CG63. baseline and differential.
GenevisibleiA2CG63. MM.

Family and domain databases

Gene3Di1.10.150.60. 1 hit.
InterProiIPR028853. ARID4B.
IPR001606. ARID_dom.
IPR016197. Chromodomain-like.
IPR012603. RBB1NT.
IPR002999. Tudor.
IPR025995. Tudor-knot.
[Graphical view]
PANTHERiPTHR13964:SF24. PTHR13964:SF24. 1 hit.
PfamiPF01388. ARID. 1 hit.
PF08169. RBB1NT. 1 hit.
PF11717. Tudor-knot. 1 hit.
[Graphical view]
SMARTiSM00501. BRIGHT. 1 hit.
SM00333. TUDOR. 2 hits.
[Graphical view]
SUPFAMiSSF46774. SSF46774. 1 hit.
SSF54160. SSF54160. 1 hit.
PROSITEiPS51011. ARID. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARI4B_MOUSE
AccessioniPrimary (citable) accession number: A2CG63
Secondary accession number(s): A2CG61
, A2CG62, A2CG64, B2RQ67, Q8BMI8, Q8BYA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: February 20, 2007
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.