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A2CAW2

- MEND_PROM3

UniProt

A2CAW2 - MEND_PROM3

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Protein

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Gene

menD

Organism
Prochlorococcus marinus (strain MIT 9303)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC).UniRule annotation

Catalytic activityi

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotation

Cofactori

Magnesium or manganese.UniRule annotation
Binds 1 thiamine pyrophosphate per subunit.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. thiamine pyrophosphate binding Source: InterPro

GO - Biological processi

  1. menaquinone biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Menaquinone biosynthesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciPMAR59922:GH54-1982-MONOMER.
UniPathwayiUPA00079.
UPA01057; UER00164.

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotation (EC:2.2.1.9UniRule annotation)
Short name:
SEPHCHC synthaseUniRule annotation
Alternative name(s):
Menaquinone biosynthesis protein MenDUniRule annotation
Gene namesi
Name:menDUniRule annotation
Ordered Locus Names:P9303_18801
OrganismiProchlorococcus marinus (strain MIT 9303)
Taxonomic identifieri59922 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000002274: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5825822-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthasePRO_0000341802Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi59922.P9303_18801.

Structurei

3D structure databases

ProteinModelPortaliA2CAW2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TPP enzyme family. MenD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1165.
HOGENOMiHOG000218359.
KOiK02551.
OrthoDBiEOG6NWBQW.

Family and domain databases

Gene3Di3.40.50.970. 2 hits.
HAMAPiMF_01659. MenD.
InterProiIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamiPF02775. TPP_enzyme_C. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004983. MenD. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00173. menD. 1 hit.

Sequencei

Sequence statusi: Complete.

A2CAW2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLRHLVLCP GSRSGPLALA AGGMARTNLL RLSTAIDERS AAFLALGFST
60 70 80 90 100
ATGTAAAVVT TSGTAVANLL PAAVEADRSC QPLLLLTADR PYRLKDCGAN
110 120 130 140 150
QTVNQETFLS PACRWIGQGP REGLHLFSTE TLESFAEEAW QRAHHPAGAV
160 170 180 190 200
HLNLPFEEPL HLSEEEQRVI WKGWSPKIPR SAPITPTNLA MAAEGTEGVT
210 220 230 240 250
DRAPFALDPF RPGVVIAGAW RGLSKDLFAF QQSLREWQAL SGWPVLADPL
260 270 280 290 300
SALPSDQPGL IRSWELLLAA GLLGSHEQLQ VLRLGPMSAS RSLEAWLKSF
310 320 330 340 350
GDGQLLITEG DSRCLDPLGL SVQWSHGLTT WWQHHHHRWI DADAASQQAT
360 370 380 390 400
QPLLKKWQII DRFAQDWLDQ QLPLQGAITE PALARWLSRL LPAELSIMLA
410 420 430 440 450
ASSPVRDWLA YADKSLFSRR CFSFRGASGI DGTLSLSMGL AMALGPTLLV
460 470 480 490 500
SGDLALLHDS NGWLLAHPQR PPLVVVLIDN GGGGIFEQLL VKKAPSEAFE
510 520 530 540 550
QLFAMPQAVD PLVLAAAHNI PHRQVACLED LPAALEWGLF QTGPVLIRVC
560 570 580
THRGQDSSMR QQLREGLVMH LQSISQNGHI DL
Length:582
Mass (Da):63,295
Last modified:July 1, 2008 - v2
Checksum:i808A6C03071EBCE5
GO

Sequence cautioni

The sequence ABM78622.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000554 Genomic DNA. Translation: ABM78622.1. Different initiation.
RefSeqiYP_001017887.1. NC_008820.1.

Genome annotation databases

EnsemblBacteriaiABM78622; ABM78622; P9303_18801.
GeneIDi4778095.
KEGGipmf:P9303_18801.
PATRICi23001255. VBIProMar17757_1936.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000554 Genomic DNA. Translation: ABM78622.1 . Different initiation.
RefSeqi YP_001017887.1. NC_008820.1.

3D structure databases

ProteinModelPortali A2CAW2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 59922.P9303_18801.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABM78622 ; ABM78622 ; P9303_18801 .
GeneIDi 4778095.
KEGGi pmf:P9303_18801.
PATRICi 23001255. VBIProMar17757_1936.

Phylogenomic databases

eggNOGi COG1165.
HOGENOMi HOG000218359.
KOi K02551.
OrthoDBi EOG6NWBQW.

Enzyme and pathway databases

UniPathwayi UPA00079 .
UPA01057 ; UER00164 .
BioCyci PMAR59922:GH54-1982-MONOMER.

Family and domain databases

Gene3Di 3.40.50.970. 2 hits.
HAMAPi MF_01659. MenD.
InterProi IPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR011766. TPP_enzyme-bd_C.
[Graphical view ]
Pfami PF02775. TPP_enzyme_C. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF004983. MenD. 1 hit.
SUPFAMi SSF52518. SSF52518. 2 hits.
TIGRFAMsi TIGR00173. menD. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MIT 9303.

Entry informationi

Entry nameiMEND_PROM3
AccessioniPrimary (citable) accession number: A2CAW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 1, 2008
Last modified: October 29, 2014
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3