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Protein

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Gene

menD

Organism
Prochlorococcus marinus (strain MIT 9303)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC).UniRule annotation

Catalytic activityi

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotation, Mn2+UniRule annotation
  • thiamine diphosphateUniRule annotationNote: Binds 1 thiamine pyrophosphate per subunit.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. manganese ion binding Source: UniProtKB-HAMAP
  4. thiamine pyrophosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. menaquinone biosynthetic process Source: InterPro
  2. phylloquinone biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciPMAR59922:GH54-1982-MONOMER.
UniPathwayiUPA00995.
UPA01057; UER00164.

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotation (EC:2.2.1.9UniRule annotation)
Short name:
SEPHCHC synthaseUniRule annotation
Gene namesi
Name:menDUniRule annotation
Ordered Locus Names:P9303_18801
OrganismiProchlorococcus marinus (strain MIT 9303)
Taxonomic identifieri59922 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000002274: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5825822-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthasePRO_0000341802Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi59922.P9303_18801.

Structurei

3D structure databases

ProteinModelPortaliA2CAW2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TPP enzyme family. MenD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1165.
HOGENOMiHOG000218359.
KOiK02551.
OrthoDBiEOG6NWBQW.

Family and domain databases

Gene3Di3.40.50.970. 2 hits.
HAMAPiMF_01659. MenD.
InterProiIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamiPF02775. TPP_enzyme_C. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004983. MenD. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00173. menD. 1 hit.

Sequencei

Sequence statusi: Complete.

A2CAW2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLRHLVLCP GSRSGPLALA AGGMARTNLL RLSTAIDERS AAFLALGFST
60 70 80 90 100
ATGTAAAVVT TSGTAVANLL PAAVEADRSC QPLLLLTADR PYRLKDCGAN
110 120 130 140 150
QTVNQETFLS PACRWIGQGP REGLHLFSTE TLESFAEEAW QRAHHPAGAV
160 170 180 190 200
HLNLPFEEPL HLSEEEQRVI WKGWSPKIPR SAPITPTNLA MAAEGTEGVT
210 220 230 240 250
DRAPFALDPF RPGVVIAGAW RGLSKDLFAF QQSLREWQAL SGWPVLADPL
260 270 280 290 300
SALPSDQPGL IRSWELLLAA GLLGSHEQLQ VLRLGPMSAS RSLEAWLKSF
310 320 330 340 350
GDGQLLITEG DSRCLDPLGL SVQWSHGLTT WWQHHHHRWI DADAASQQAT
360 370 380 390 400
QPLLKKWQII DRFAQDWLDQ QLPLQGAITE PALARWLSRL LPAELSIMLA
410 420 430 440 450
ASSPVRDWLA YADKSLFSRR CFSFRGASGI DGTLSLSMGL AMALGPTLLV
460 470 480 490 500
SGDLALLHDS NGWLLAHPQR PPLVVVLIDN GGGGIFEQLL VKKAPSEAFE
510 520 530 540 550
QLFAMPQAVD PLVLAAAHNI PHRQVACLED LPAALEWGLF QTGPVLIRVC
560 570 580
THRGQDSSMR QQLREGLVMH LQSISQNGHI DL
Length:582
Mass (Da):63,295
Last modified:July 1, 2008 - v2
Checksum:i808A6C03071EBCE5
GO

Sequence cautioni

The sequence ABM78622.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000554 Genomic DNA. Translation: ABM78622.1. Different initiation.
RefSeqiYP_001017887.1. NC_008820.1.

Genome annotation databases

EnsemblBacteriaiABM78622; ABM78622; P9303_18801.
GeneIDi4778095.
KEGGipmf:P9303_18801.
PATRICi23001255. VBIProMar17757_1936.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000554 Genomic DNA. Translation: ABM78622.1. Different initiation.
RefSeqiYP_001017887.1. NC_008820.1.

3D structure databases

ProteinModelPortaliA2CAW2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi59922.P9303_18801.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABM78622; ABM78622; P9303_18801.
GeneIDi4778095.
KEGGipmf:P9303_18801.
PATRICi23001255. VBIProMar17757_1936.

Phylogenomic databases

eggNOGiCOG1165.
HOGENOMiHOG000218359.
KOiK02551.
OrthoDBiEOG6NWBQW.

Enzyme and pathway databases

UniPathwayiUPA00995.
UPA01057; UER00164.
BioCyciPMAR59922:GH54-1982-MONOMER.

Family and domain databases

Gene3Di3.40.50.970. 2 hits.
HAMAPiMF_01659. MenD.
InterProiIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamiPF02775. TPP_enzyme_C. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004983. MenD. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00173. menD. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MIT 9303.

Entry informationi

Entry nameiMEND_PROM3
AccessioniPrimary (citable) accession number: A2CAW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 1, 2008
Last modified: February 4, 2015
This is version 56 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.