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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Prochlorococcus marinus (strain MIT 9303)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei633 – 6331ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPMAR59922:GH54-1596-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:P9303_15061
OrganismiProchlorococcus marinus (strain MIT 9303)
Taxonomic identifieri59922 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000002274: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 878878Leucine--tRNA ligasePRO_1000009393Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi59922.P9303_15061.

Structurei

3D structure databases

ProteinModelPortaliA2C9U0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi56 – 6611"HIGH" regionAdd
BLAST
Motifi630 – 6345"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2C9U0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTESFPSTSA SSHSSARYDP IELETRWQKE WLRQGLDRTP VAETNQKRFY
60 70 80 90 100
ALSMFPYPSG KLHMGHVRNY VITDVIARVQ RMRGDAVLHP MGWDAFGLPA
110 120 130 140 150
ENAAIARNVD PGDWTDQNIA QMRAQLDRLG LSIDWDRQQA TCHQDYYRWT
160 170 180 190 200
QWLFLELFAG GLAYQKEATV NWDPIDKTVL ANEQVDGEGR SWRSGALVEQ
210 220 230 240 250
RQLKQWFLRI TDYADALIDD LDELTGWPER VRTMQANWIG RSHGAEIKFR
260 270 280 290 300
VAGQTNSIIT VFTTRPDTLH GASYVVLAPE HPLVEALTSP QQRIAVTAFC
310 320 330 340 350
DLISQLSVKD RTAEDQPKRG VPIGAQVINP VNGESLPVWI ADYVLADYGS
360 370 380 390 400
GAVMGVPAHD ERDFIFARSH ELPIRIVVQM PDSDEHHNDG QAWTGAGVLV
410 420 430 440 450
NSGAFDGLST EEGKVAITTH GASKGWAQSK VQYRLRDWLI SRQRYWGCPI
460 470 480 490 500
PIIHCASCGI VPVPQEDLPV TLPRDIDLSG KGGSPLAQEQ DWVEVKCPIC
510 520 530 540 550
GEKAHRETDT MDTFMCSSWY YLRFADPLNS QRPFDKDIVD EWLPVDQYVG
560 570 580 590 600
GIEHAILHLL YARFFTKALH DRNLIGFKEP FNTLLTQGMV QGLTYRNTKN
610 620 630 640 650
GSYISPELVS DEGDPRDPES GDKLEILFEK MSKSKYNGVD PAVVIDRYGA
660 670 680 690 700
DTARMFILFK APPEKDLEWD DADVEGQFRF LQRLIRLIDS FVWPKTDEDN
710 720 730 740 750
ASISSANLTI SSADLSEEEI NMRRATHMAI EAITEDLSGD IQLNTAISEL
760 770 780 790 800
MKLSNSLGGK LDKVRTEVAA EALSVLVRLM APFAPHLAEE FWMKLHGQGS
810 820 830 840 850
IHQQSWPIID PSALVLETIE LVIQVKGKVR GTIQVPANAD KTALEELALK
860 870
SDIAVKWLEG QSPRRVIIVP GKLVNLVP
Length:878
Mass (Da):98,546
Last modified:February 20, 2007 - v1
Checksum:iD9528F8690422D93
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000554 Genomic DNA. Translation: ABM78250.1.
RefSeqiYP_001017515.1. NC_008820.1.

Genome annotation databases

EnsemblBacteriaiABM78250; ABM78250; P9303_15061.
GeneIDi4777973.
KEGGipmf:P9303_15061.
PATRICi23000490. VBIProMar17757_1556.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000554 Genomic DNA. Translation: ABM78250.1.
RefSeqiYP_001017515.1. NC_008820.1.

3D structure databases

ProteinModelPortaliA2C9U0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi59922.P9303_15061.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABM78250; ABM78250; P9303_15061.
GeneIDi4777973.
KEGGipmf:P9303_15061.
PATRICi23000490. VBIProMar17757_1556.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciPMAR59922:GH54-1596-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MIT 9303.

Entry informationi

Entry nameiSYL_PROM3
AccessioniPrimary (citable) accession number: A2C9U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 20, 2007
Last modified: January 7, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.