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A2C9U0 (SYL_PROM3) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:P9303_15061
OrganismProchlorococcus marinus (strain MIT 9303) [Complete proteome] [HAMAP]
Taxonomic identifier59922 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus

Protein attributes

Sequence length878 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 878878Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009393

Regions

Motif56 – 6611"HIGH" region HAMAP-Rule MF_00049
Motif630 – 6345"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6331ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A2C9U0 [UniParc].

Last modified February 20, 2007. Version 1.
Checksum: D9528F8690422D93

FASTA87898,546
        10         20         30         40         50         60 
MTESFPSTSA SSHSSARYDP IELETRWQKE WLRQGLDRTP VAETNQKRFY ALSMFPYPSG 

        70         80         90        100        110        120 
KLHMGHVRNY VITDVIARVQ RMRGDAVLHP MGWDAFGLPA ENAAIARNVD PGDWTDQNIA 

       130        140        150        160        170        180 
QMRAQLDRLG LSIDWDRQQA TCHQDYYRWT QWLFLELFAG GLAYQKEATV NWDPIDKTVL 

       190        200        210        220        230        240 
ANEQVDGEGR SWRSGALVEQ RQLKQWFLRI TDYADALIDD LDELTGWPER VRTMQANWIG 

       250        260        270        280        290        300 
RSHGAEIKFR VAGQTNSIIT VFTTRPDTLH GASYVVLAPE HPLVEALTSP QQRIAVTAFC 

       310        320        330        340        350        360 
DLISQLSVKD RTAEDQPKRG VPIGAQVINP VNGESLPVWI ADYVLADYGS GAVMGVPAHD 

       370        380        390        400        410        420 
ERDFIFARSH ELPIRIVVQM PDSDEHHNDG QAWTGAGVLV NSGAFDGLST EEGKVAITTH 

       430        440        450        460        470        480 
GASKGWAQSK VQYRLRDWLI SRQRYWGCPI PIIHCASCGI VPVPQEDLPV TLPRDIDLSG 

       490        500        510        520        530        540 
KGGSPLAQEQ DWVEVKCPIC GEKAHRETDT MDTFMCSSWY YLRFADPLNS QRPFDKDIVD 

       550        560        570        580        590        600 
EWLPVDQYVG GIEHAILHLL YARFFTKALH DRNLIGFKEP FNTLLTQGMV QGLTYRNTKN 

       610        620        630        640        650        660 
GSYISPELVS DEGDPRDPES GDKLEILFEK MSKSKYNGVD PAVVIDRYGA DTARMFILFK 

       670        680        690        700        710        720 
APPEKDLEWD DADVEGQFRF LQRLIRLIDS FVWPKTDEDN ASISSANLTI SSADLSEEEI 

       730        740        750        760        770        780 
NMRRATHMAI EAITEDLSGD IQLNTAISEL MKLSNSLGGK LDKVRTEVAA EALSVLVRLM 

       790        800        810        820        830        840 
APFAPHLAEE FWMKLHGQGS IHQQSWPIID PSALVLETIE LVIQVKGKVR GTIQVPANAD 

       850        860        870 
KTALEELALK SDIAVKWLEG QSPRRVIIVP GKLVNLVP 

« Hide

References

[1]"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus."
Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W.
PLoS Genet. 3:2515-2528(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MIT 9303.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000554 Genomic DNA. Translation: ABM78250.1.
RefSeqYP_001017515.1. NC_008820.1.

3D structure databases

ProteinModelPortalA2C9U0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING59922.P9303_15061.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM78250; ABM78250; P9303_15061.
GeneID4777973.
KEGGpmf:P9303_15061.
PATRIC23000490. VBIProMar17757_1556.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycPMAR59922:GH54-1596-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PROM3
AccessionPrimary (citable) accession number: A2C9U0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 20, 2007
Last modified: May 14, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries