A2C7W3 (GLO2_PROM3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	P9303_08221

Organism

Prochlorococcus marinus (strain MIT 9303) [Complete proteome] [HAMAP]

Taxonomic identifier

59922 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_0000309678
Sites
	Metal binding	66	1	Zinc 1 By similarity
	Metal binding	68	1	Zinc 1 By similarity
	Metal binding	70	1	Zinc 2 By similarity
	Metal binding	71	1	Zinc 2 By similarity
	Metal binding	125	1	Zinc 1 By similarity
	Metal binding	150	1	Zinc 1 By similarity
	Metal binding	150	1	Zinc 2 By similarity
	Metal binding	188	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2C7W3 [UniParc]. Last modified February 20, 2007. Version 1. Checksum: C8610411863A28C1	FASTA	260	29,082
	10 20 30 40 50 60 MEATSTAATS DKQRSSIHAL PVLQDNIIWI WIKGDQAVVV DPAIAEPVKT WLQTRKLSLA 70 80 90 100 110 120 AVLQTHHHAD HIGGTLELLR DWPNAAVVAA ADDRDRIPFQ TISVRDRDKI SLLNSSVEVL 130 140 150 160 170 180 AVAGHTRAHI AYYLPTNKED REDPAVFCGD TLFGAGCGRL FEGTAEDMFK ALQRLCCLPA 190 200 210 220 230 240 KTRVYCAHEY TEANLRWASA LHPEDIAISE RLVDVSSRRQ RGALSLPSSI SEEQRTNLFV 250 260 RAQNSKELAE LRQHKDQWRN

References

[1]

"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus." Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W. PLoS Genet. 3:2515-2528(2007) [PubMed: 18159947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MIT 9303.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000554 Genomic DNA. Translation: ABM77573.1.
RefSeq	YP_001016838.1. NC_008820.1.
3D structure databases
ProteinModelPortal	A2C7W3.
ModBase	Search...
Protein-protein interaction databases
STRING	A2C7W3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4777903.
GenomeReviews	Gene locus P9303_08221 in contig CP000554_GR.
KEGG	pmf:P9303_08221.
PATRIC	22999064. VBIProMar17757_0850.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0491.
HOGENOM	HBG753931.
OMA	FLCRTDN.
PhylomeDB	A2C7W3.
ProtClustDB	CLSK647185.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_PROM3

Accession

Primary (citable) accession number: A2C7W3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	February 20, 2007
Last modified:	December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program