Reviewed, UniProtKB/Swiss-Prot A2C764 (GLUQ_PROM3)

Names and origin

Protein names

Recommended name:
    Glutamyl-Q tRNA(Asp) synthetase
      Short name=Glu-Q-RSs
    EC=6.1.1.-

Gene names

Name:	gluQ
Ordered Locus Names:	P9303_05721

Organism

Prochlorococcus marinus (strain MIT 9303) [Complete proteome] [HAMAP]

Taxonomic identifier

59922 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5-dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble position of the QUC anticodon By similarity.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily.

Ontologies

Keywords
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Glutamyl-Q tRNA(Asp) synthetase HAMAP MF_01428		PRO_1000024360
Regions
	Region	29 – 33	5	Glutamate binding By similarity
	Motif	32 – 42	11	"HIGH" region HAMAP MF_01428
	Motif	244 – 248	5	"KMSKS" region HAMAP MF_01428
Sites
	Metal binding	121	1	Zinc By similarity
	Metal binding	123	1	Zinc By similarity
	Metal binding	141	1	Zinc By similarity
	Metal binding	145	1	Zinc By similarity
	Binding site	65	1	Glutamate By similarity
	Binding site	188	1	Glutamate By similarity
	Binding site	206	1	Glutamate By similarity
	Binding site	247	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2C764-1 [UniParc]. Last modified February 20, 2007. Version 1. Checksum: 30E6D5280341ED46	FASTA	306	34,070
	10 20 30 40 50 60 MATSSELPDH LYEQMEAGQR LRNQGYRGRF APSPTGPLHL GNLCTALVSW LQARLANGAW 70 80 90 100 110 120 LLRVDDLDQP RNRVGAVESL QQDLHWLGLD WDGPVVFQSR RRGLYNSFLS ALRRQGKLYA 130 140 150 160 170 180 CRCSRRMLAD ISAPAGRHLV YPGTCRDLEL FWGWHEGRLP SWRLRVSKEF SHTSGDVILR 190 200 210 220 230 240 RADGFIAYHL ATVVDELTLG ISEVVRGEDL LVAMNAQLAL INEISARPVI YRHVPLLCDD 250 260 270 280 290 300 QGRKLAKREG HAGLDSLRSE GLGPSHVVGW LAASQSLVPF GAELTAGELL SELKKKEGVL KSVLKP

References

[1]

"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus." Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W. PLoS Genet. 3:2515-2528(2007) [PubMed: 18159947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000554 Genomic DNA. Translation: ABM77324.1.
RefSeq	YP_001016589.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A2C764.
Genome annotation databases
GeneID	4778302.
GenomeReviews	Gene locus P9303_05721 in contig CP000554_GR.
KEGG	pmf:P9303_05721.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	PWPRDPD.
Family and domain databases
HAMAP	MF_01428. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 2 hits. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLUQ_PROM3

Accession

Primary (citable) accession number: A2C764

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 20, 2007
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)