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A2C3I4

- BIOB_PROM1

UniProt

A2C3I4 - BIOB_PROM1

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Protein
Biotin synthase
Gene
bioB, NATL1_14871
Organism
Prochlorococcus marinus (strain NATL1A)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi82 – 821Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi86 – 861Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi89 – 891Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi126 – 1261Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi158 – 1581Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi218 – 2181Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi290 – 2901Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPMAR167555:GI3K-1517-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:NATL1_14871
OrganismiProchlorococcus marinus (strain NATL1A)
Taxonomic identifieri167555 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000002592: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 345345Biotin synthaseUniRule annotation
PRO_0000381537Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi167555.NATL1_14871.

Structurei

3D structure databases

ProteinModelPortaliA2C3I4.
SMRiA2C3I4. Positions 33-343.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A2C3I4-1 [UniParc]FASTAAdd to Basket

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MTLINPNIQE SNKLKFKDES YLDFNSINGG DIRHDWSSEE IKEILDLPLM    50
DLLWRAQIVH RSYNPGYKVQ LASLLSVKTG GCSEDCAYCP QSVHNETTVQ 100
PNPVIEVESV LDRARAAKDA GADRFCMGWA WREIKDGNAF DSMLEMVRGV 150
RELGLEACVT AGMITDSQAS RLAEAGLTAY NHNLDTSPEH YSKIISTRTY 200
QDRLETLRRV RMAGITVCCG GIIGMGESVS DRASLLKVLA TLDPHPESVP 250
INALVAVEGT PMEDLSSIDP LEMVRMVATA RVIMPKSRIR LSAGRQQLGR 300
EAQILCLQSG ADSIFYGDTL LTTSNPEVEA DRKLLADAGI TANFS 345
Length:345
Mass (Da):37,938
Last modified:February 20, 2007 - v1
Checksum:i5DC1EA58E189ECBA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000553 Genomic DNA. Translation: ABM76044.1.
RefSeqiWP_011824069.1. NC_008819.1.
YP_001015309.1. NC_008819.1.

Genome annotation databases

EnsemblBacteriaiABM76044; ABM76044; NATL1_14871.
GeneIDi4779672.
KEGGipme:NATL1_14871.
PATRICi23020577. VBIProMar31285_1511.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000553 Genomic DNA. Translation: ABM76044.1 .
RefSeqi WP_011824069.1. NC_008819.1.
YP_001015309.1. NC_008819.1.

3D structure databases

ProteinModelPortali A2C3I4.
SMRi A2C3I4. Positions 33-343.
ModBasei Search...

Protein-protein interaction databases

STRINGi 167555.NATL1_14871.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABM76044 ; ABM76044 ; NATL1_14871 .
GeneIDi 4779672.
KEGGi pme:NATL1_14871.
PATRICi 23020577. VBIProMar31285_1511.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci PMAR167555:GI3K-1517-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NATL1A.

Entry informationi

Entry nameiBIOB_PROM1
AccessioniPrimary (citable) accession number: A2C3I4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 20, 2007
Last modified: September 3, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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