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A2C3I4

- BIOB_PROM1

UniProt

A2C3I4 - BIOB_PROM1

Protein

Biotin synthase

Gene

bioB

Organism
Prochlorococcus marinus (strain NATL1A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (20 Feb 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi82 – 821Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi86 – 861Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi89 – 891Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi126 – 1261Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi158 – 1581Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi218 – 2181Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi290 – 2901Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciPMAR167555:GI3K-1517-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:NATL1_14871
    OrganismiProchlorococcus marinus (strain NATL1A)
    Taxonomic identifieri167555 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
    ProteomesiUP000002592: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 345345Biotin synthasePRO_0000381537Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi167555.NATL1_14871.

    Structurei

    3D structure databases

    ProteinModelPortaliA2C3I4.
    SMRiA2C3I4. Positions 33-343.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiRIMMPAS.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A2C3I4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTLINPNIQE SNKLKFKDES YLDFNSINGG DIRHDWSSEE IKEILDLPLM    50
    DLLWRAQIVH RSYNPGYKVQ LASLLSVKTG GCSEDCAYCP QSVHNETTVQ 100
    PNPVIEVESV LDRARAAKDA GADRFCMGWA WREIKDGNAF DSMLEMVRGV 150
    RELGLEACVT AGMITDSQAS RLAEAGLTAY NHNLDTSPEH YSKIISTRTY 200
    QDRLETLRRV RMAGITVCCG GIIGMGESVS DRASLLKVLA TLDPHPESVP 250
    INALVAVEGT PMEDLSSIDP LEMVRMVATA RVIMPKSRIR LSAGRQQLGR 300
    EAQILCLQSG ADSIFYGDTL LTTSNPEVEA DRKLLADAGI TANFS 345
    Length:345
    Mass (Da):37,938
    Last modified:February 20, 2007 - v1
    Checksum:i5DC1EA58E189ECBA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000553 Genomic DNA. Translation: ABM76044.1.
    RefSeqiWP_011824069.1. NC_008819.1.
    YP_001015309.1. NC_008819.1.

    Genome annotation databases

    EnsemblBacteriaiABM76044; ABM76044; NATL1_14871.
    GeneIDi4779672.
    KEGGipme:NATL1_14871.
    PATRICi23020577. VBIProMar31285_1511.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000553 Genomic DNA. Translation: ABM76044.1 .
    RefSeqi WP_011824069.1. NC_008819.1.
    YP_001015309.1. NC_008819.1.

    3D structure databases

    ProteinModelPortali A2C3I4.
    SMRi A2C3I4. Positions 33-343.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 167555.NATL1_14871.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABM76044 ; ABM76044 ; NATL1_14871 .
    GeneIDi 4779672.
    KEGGi pme:NATL1_14871.
    PATRICi 23020577. VBIProMar31285_1511.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi RIMMPAS.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci PMAR167555:GI3K-1517-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NATL1A.

    Entry informationi

    Entry nameiBIOB_PROM1
    AccessioniPrimary (citable) accession number: A2C3I4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: February 20, 2007
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3