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A2C3I4 (BIOB_PROM1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:NATL1_14871
OrganismProchlorococcus marinus (strain NATL1A) [Complete proteome] [HAMAP]
Taxonomic identifier167555 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus

Protein attributes

Sequence length345 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 345345Biotin synthase HAMAP-Rule MF_01694
PRO_0000381537

Sites

Metal binding821Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding861Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding891Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1261Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1581Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2181Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2901Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A2C3I4 [UniParc].

Last modified February 20, 2007. Version 1.
Checksum: 5DC1EA58E189ECBA

FASTA34537,938
        10         20         30         40         50         60 
MTLINPNIQE SNKLKFKDES YLDFNSINGG DIRHDWSSEE IKEILDLPLM DLLWRAQIVH 

        70         80         90        100        110        120 
RSYNPGYKVQ LASLLSVKTG GCSEDCAYCP QSVHNETTVQ PNPVIEVESV LDRARAAKDA 

       130        140        150        160        170        180 
GADRFCMGWA WREIKDGNAF DSMLEMVRGV RELGLEACVT AGMITDSQAS RLAEAGLTAY 

       190        200        210        220        230        240 
NHNLDTSPEH YSKIISTRTY QDRLETLRRV RMAGITVCCG GIIGMGESVS DRASLLKVLA 

       250        260        270        280        290        300 
TLDPHPESVP INALVAVEGT PMEDLSSIDP LEMVRMVATA RVIMPKSRIR LSAGRQQLGR 

       310        320        330        340 
EAQILCLQSG ADSIFYGDTL LTTSNPEVEA DRKLLADAGI TANFS 

« Hide

References

[1]"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus."
Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W.
PLoS Genet. 3:2515-2528(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NATL1A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000553 Genomic DNA. Translation: ABM76044.1.
RefSeqYP_001015309.1. NC_008819.1.

3D structure databases

ProteinModelPortalA2C3I4.
SMRA2C3I4. Positions 33-343.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING167555.NATL1_14871.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM76044; ABM76044; NATL1_14871.
GeneID4779672.
KEGGpme:NATL1_14871.
PATRIC23020577. VBIProMar31285_1511.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMARIMMPAS.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycPMAR167555:GI3K-1517-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_PROM1
AccessionPrimary (citable) accession number: A2C3I4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 20, 2007
Last modified: May 14, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways