A2C116 (GLO2_PROM1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	NATL1_06141

Organism

Prochlorococcus marinus (strain NATL1A) [Complete proteome] [HAMAP]

Taxonomic identifier

167555 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_0000309681
Sites
	Metal binding	59	1	Zinc 1 By similarity
	Metal binding	61	1	Zinc 1 By similarity
	Metal binding	63	1	Zinc 2 By similarity
	Metal binding	64	1	Zinc 2 By similarity
	Metal binding	118	1	Zinc 1 By similarity
	Metal binding	141	1	Zinc 1 By similarity
	Metal binding	141	1	Zinc 2 By similarity
	Metal binding	179	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2C116 [UniParc]. Last modified February 20, 2007. Version 1. Checksum: 10D991ACD04B5AAE	FASTA	251	28,692
	10 20 30 40 50 60 MLGEKSDFTI HPINVLQDNI VWVWVHNCNA VVVDPSISGP VEKWLLEKNL SLKAILQTHH 70 80 90 100 110 120 HDDHIGGTQK LIKTWPEAKV VASKKEHKRI PFQTFSVDDN DIFNLMDAEI KVIEVHGHTD 130 140 150 160 170 180 NHIAFYISKQ NAKCNILFPG DTLFGGGCGR LLEGSPVQMF ESLYKLNSLP ENTEIYPAHE 190 200 210 220 230 240 YTESNLKWAL SLEPGNISII ERLKLIQKKL QKGMSSLPST LSEERKTNLF LIAENVEKFT 250 MLRKHKDRWK C

References

[1]

"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus." Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W. PLoS Genet. 3:2515-2528(2007) [PubMed: 18159947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NATL1A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000553 Genomic DNA. Translation: ABM75176.1.
RefSeq	YP_001014441.1. NC_008819.1.
3D structure databases
ProteinModelPortal	A2C116.
ModBase	Search...
Protein-protein interaction databases
STRING	A2C116.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4779603.
GenomeReviews	Gene locus NATL1_06141 in contig CP000553_GR.
KEGG	pme:NATL1_06141.
PATRIC	23018789. VBIProMar31285_0626.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0491.
HOGENOM	HBG753931.
OMA	FLCRTDN.
PhylomeDB	A2C116.
ProtClustDB	CLSK716142.
Enzyme and pathway databases
BioCyc	PMAR167555:NATL1_06141-MONOMER.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_PROM1

Accession

Primary (citable) accession number: A2C116

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	February 20, 2007
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program