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Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Prochlorococcus marinus (strain NATL1A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi347 – 3471Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi407 – 4071Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi410 – 4101Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 3-isopropylmalate dehydratase activity Source: UniProtKB-HAMAP
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. leucine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciPMAR167555:GI3K-339-MONOMER.
UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomeraseUniRule annotation
Short name:
IPMIUniRule annotation
Isopropylmalate isomeraseUniRule annotation
Gene namesi
Name:leuCUniRule annotation
Ordered Locus Names:NATL1_03341
OrganismiProchlorococcus marinus (strain NATL1A)
Taxonomic identifieri167555 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000002592: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4694693-isopropylmalate dehydratase large subunitPRO_1000063583Add
BLAST

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotation

Protein-protein interaction databases

STRINGi167555.NATL1_03341.

Structurei

3D structure databases

ProteinModelPortaliA2C088.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0065.
HOGENOMiHOG000226972.
KOiK01703.
OMAiDKVWDLH.
OrthoDBiEOG600DP5.

Family and domain databases

Gene3Di3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01026. LeuC_type1.
InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR00170. leuC. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2C088-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSRTLYDKV WNFHQVKELP GGSTQLFIGL HLIHEVTSPQ AFSALNEKKL
60 70 80 90 100
GVKFPNLTVA TVDHIVPTSN QQRPFSDPLA EEMLSTLEKN CKTHGIKFHG
110 120 130 140 150
IGSNSQGVVH VMAPELGLTQ PGMTVACGDS HTSTHGAFGA IAFGIGTSQV
160 170 180 190 200
RDVLASQSLA MNKLKVRRIW VEGELQKGVY AKDLILHIIR HLGVKGGVGF
210 220 230 240 250
AYEFAGPAIE KLSMEGRMTI CNMAIEGGAR CGYINPDETT FKYLKGKEHA
260 270 280 290 300
PKGQEWDKAI SWWKSLASDS KATFDDEIQL DGSSIEPTVT WGITPGQGIS
310 320 330 340 350
IKETIPNPEF LPKNEQQIAK DACKYMNLKP DEPIEGQSID VCFIGSCTNG
360 370 380 390 400
RLSDLQEASK IVKGNTVADG IRAFVVPGSQ KVAKEAKEKG LDKIFLKAGF
410 420 430 440 450
EWREPGCSMC LAMNPDKLEG RQISASSSNR NFKGRQGSAK GRTLLMSPAM
460
VAAAAINGRV TDVRKFLQE
Length:469
Mass (Da):51,032
Last modified:February 20, 2007 - v1
Checksum:i5289B9679BD1EB9E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000553 Genomic DNA. Translation: ABM74898.1.
RefSeqiYP_001014163.1. NC_008819.1.

Genome annotation databases

EnsemblBacteriaiABM74898; ABM74898; NATL1_03341.
GeneIDi4779767.
KEGGipme:NATL1_03341.
PATRICi23018193. VBIProMar31285_0341.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000553 Genomic DNA. Translation: ABM74898.1.
RefSeqiYP_001014163.1. NC_008819.1.

3D structure databases

ProteinModelPortaliA2C088.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi167555.NATL1_03341.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABM74898; ABM74898; NATL1_03341.
GeneIDi4779767.
KEGGipme:NATL1_03341.
PATRICi23018193. VBIProMar31285_0341.

Phylogenomic databases

eggNOGiCOG0065.
HOGENOMiHOG000226972.
KOiK01703.
OMAiDKVWDLH.
OrthoDBiEOG600DP5.

Enzyme and pathway databases

UniPathwayiUPA00048; UER00071.
BioCyciPMAR167555:GI3K-339-MONOMER.

Family and domain databases

Gene3Di3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01026. LeuC_type1.
InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR00170. leuC. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NATL1A.

Entry informationi

Entry nameiLEUC_PROM1
AccessioniPrimary (citable) accession number: A2C088
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 20, 2007
Last modified: January 7, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.