A2BYW5 (HEM6_PROM5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Coproporphyrinogen-III oxidase, aerobic
Short name=Coprogen oxidase
Short name=Coproporphyrinogenase
EC=1.3.3.3

Gene names

Name:	hemF
Ordered Locus Names:	P9515_17691

Organism

Prochlorococcus marinus (strain MIT 9515) [Complete proteome] [HAMAP]

Taxonomic identifier

167542 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity. HAMAP MF_00333
Catalytic activity	Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP MF_00333
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP MF_00333
Subunit structure	Homodimer By similarity. HAMAP MF_00333
Subcellular location	Cytoplasm By similarity HAMAP MF_00333.
Sequence similarities	Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: EC protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	Coproporphyrinogen-III oxidase, aerobic HAMAP MF_00333		PRO_1000019478
Regions
	Region	54 – 63	10	Important for dimerization By similarity
	Region	114 – 116	3	Substrate binding By similarity
	Region	266 – 301	36	Important for dimerization By similarity
	Region	284 – 289	6	Substrate binding By similarity
Sites
	Active site	112	1	Proton donor By similarity
	Binding site	98	1	Substrate By similarity
	Site	176	1	Important for dimerization By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2BYW5 [UniParc]. Last modified February 20, 2007. Version 1. Checksum: A4FF00395B216F33	FASTA	342	39,764
	10 20 30 40 50 60 MSKEPPNNSR EKTKNLLLKL QDNICKNLEN IDGKAKFTEE SWLREEGGGG RSRVLKNGSI 70 80 90 100 110 120 FEQAGVNFSE VYGKELPQSI ISQRPEAKGH EWFATGTSMV LHPKNPFIPT VHLNYRYFEA 130 140 150 160 170 180 GPVWWFGGGA DLTPYYPYLS DVRHFHKEHS NACEKVNKKL HLVFKPWCDE YFFLKHRNES 190 200 210 220 230 240 RGIGGIFYDY QDGSGNIYKG SNKDGNAYKE SNNIGELNLN WNNLFALAEN CGEAFLSSYQ 250 260 270 280 290 300 PIIEKRVSQN YTKEEREFQL YRRGRYVEFN LVWDRGTIFG LQTNGRTESI LMSLPPLARW 310 320 330 340 EYGYKAKQGS REDLLTKIFT RPQDWQNDKV LEEFCLENNI FD

References

[1]

"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus." Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W. PLoS Genet. 3:2515-2528(2007) [PubMed: 18159947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MIT 9515.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000552 Genomic DNA. Translation: ABM72976.1.
RefSeq	YP_001012083.1. NC_008817.1.
3D structure databases
ProteinModelPortal	A2BYW5.
ModBase	Search...
Protein-protein interaction databases
STRING	A2BYW5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4719892.
GenomeReviews	Gene locus P9515_17691 in contig CP000552_GR.
KEGG	pmc:P9515_17691.
PATRIC	23017220. VBIProMar113831_1819.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0408.
HOGENOM	HBG631180.
OMA	VFKPWCD.
PhylomeDB	A2BYW5.
ProtClustDB	PRK05330.
Enzyme and pathway databases
BioCyc	PMAR167542:P9515ORF_1845-MONOMER.
Family and domain databases
HAMAP	MF_00333. Coprogen_oxidas. [Tree]
InterPro	IPR001260. Coprogen_oxidase_aer. IPR018375. Coprogen_oxidase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
KO	K00228.
PANTHER	PTHR10755. Coprogen_oxidas. 1 hit.
Pfam	PF01218. Coprogen_oxidas. 1 hit. [Graphical view]
PIRSF	PIRSF000166. Coproporphyri_ox. 1 hit.
PRINTS	PR00073. COPRGNOXDASE.
SUPFAM	SSF102886. Coprogen_oxidas. 1 hit.
PROSITE	PS01021. COPROGEN_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM6_PROM5

Accession

Primary (citable) accession number: A2BYW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 20, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program