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Protein

3-methyl-2-oxobutanoate hydroxymethyltransferase

Gene

panB

Organism
Prochlorococcus marinus (strain MIT 9515)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: (R)-pantothenate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB)
  2. no protein annotated in this organism
This subpathway is part of the pathway (R)-pantothenate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate, the pathway (R)-pantothenate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi42MagnesiumUniRule annotation1
Metal bindingi86MagnesiumUniRule annotation1
Binding sitei86Alpha-ketoisovalerateUniRule annotation1
Binding sitei116Alpha-ketoisovalerateUniRule annotation1
Metal bindingi118MagnesiumUniRule annotation1
Active sitei185Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processPantothenate biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciPMAR167542:G1G7Y-1436-MONOMER
UniPathwayiUPA00028; UER00003

Names & Taxonomyi

Protein namesi
Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferaseUniRule annotation (EC:2.1.2.11UniRule annotation)
Alternative name(s):
Ketopantoate hydroxymethyltransferaseUniRule annotation
Short name:
KPHMTUniRule annotation
Gene namesi
Name:panBUniRule annotation
Ordered Locus Names:P9515_14711
OrganismiProchlorococcus marinus (strain MIT 9515)
Taxonomic identifieri167542 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesProchloraceaeProchlorococcus
Proteomesi
  • UP000001589 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002973271 – 2573-methyl-2-oxobutanoate hydroxymethyltransferaseAdd BLAST257

Proteomic databases

PRIDEiA2BY17

Interactioni

Subunit structurei

Homodecamer; pentamer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi167542.P9515_14711

Structurei

3D structure databases

SMRiA2BY17
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni42 – 43Alpha-ketoisovalerate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the PanB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCG Bacteria
COG0413 LUCA
HOGENOMiHOG000078427
KOiK00606
OMAiGHIGLMP
OrthoDBiPOG091H02K8

Family and domain databases

CDDicd06557 KPHMT-like, 1 hit
HAMAPiMF_00156 PanB, 1 hit
InterProiView protein in InterPro
IPR003700 Pantoate_hydroxy_MeTrfase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
PANTHERiPTHR20881 PTHR20881, 1 hit
PfamiView protein in Pfam
PF02548 Pantoate_transf, 1 hit
PIRSFiPIRSF000388 Pantoate_hydroxy_MeTrfase, 1 hit
SUPFAMiSSF51621 SSF51621, 1 hit
TIGRFAMsiTIGR00222 panB, 1 hit

Sequencei

Sequence statusi: Complete.

A2BY17-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPSELVKYK KNSQKIIALT AWDSISGSLA EQSGADIVLV GDSLAMVCLG
60 70 80 90 100
YKSTLPVTLE NMIYHTNAVS RGFSREIEEH SLLVCDMPFL TYQCGEDKAV
110 120 130 140 150
EYAGKIIKNT YAKAVKIEGA EPEVQTVISR LIRMGIPVMG HLGLTPQSYL
160 170 180 190 200
NLGLKKQGLK KESYEKIKRD ALSLEKLGCF SIVLEHIPEL LAKEIQTDLE
210 220 230 240 250
IPTIGIGAGV FCDGQVRVTA DLLGLSDKQP PFCKPIVDGK KYFGEKLKEW

VTSERLS
Length:257
Mass (Da):28,323
Last modified:February 20, 2007 - v1
Checksum:iBF7FB1375408B5DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000552 Genomic DNA Translation: ABM72678.1
RefSeqiWP_011820775.1, NC_008817.1

Genome annotation databases

EnsemblBacteriaiABM72678; ABM72678; P9515_14711
KEGGipmc:P9515_14711

Similar proteinsi

Entry informationi

Entry nameiPANB_PROM5
AccessioniPrimary (citable) accession number: A2BY17
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: February 20, 2007
Last modified: May 23, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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