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A2BY13 (DDL_PROM5) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:P9515_14671
OrganismProchlorococcus marinus (strain MIT 9515) [Complete proteome] [HAMAP]
Taxonomic identifier167542 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus

Protein attributes

Sequence length355 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 355355D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000030481

Regions

Domain143 – 350208ATP-grasp
Nucleotide binding178 – 23356ATP By similarity

Sites

Metal binding3031Magnesium or manganese 1 By similarity
Metal binding3171Magnesium or manganese 1 By similarity
Metal binding3171Magnesium or manganese 2 By similarity
Metal binding3191Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A2BY13 [UniParc].

Last modified February 20, 2007. Version 1.
Checksum: 95D6FD34D364E3D1

FASTA35539,960
        10         20         30         40         50         60 
MVRNEKKCIG IIFGGTSNEH YVSISSAKTV FKALISRTNK ELFRIKAFYI NKHGVWIDSD 

        70         80         90        100        110        120 
LSLEILRENS GNNIFEKYQE LPNRKINFLN NLEFQNIDIW FPLLHGCNGE DGAIHGLLKF 

       130        140        150        160        170        180 
TQKPLIGCGI SGSAIGMDKI LMKQIFSNLS IPQVNFLAIQ NYDLSDDNVK DNLSVEIIEK 

       190        200        210        220        230        240 
LNLPVFVKPA NSGSSLGISK AKNKSEIIKA LQKAWEIDSR IVIEEGLNVR ELECGIIGNL 

       250        260        270        280        290        300 
KLSTSKIGEV SYSSDWYDYD SKYSTDNKII IPADIDSQIS EQIKDLAIRS CRALNIYGFA 

       310        320        330        340        350 
RVDFFLEKIS RKIFLNEINT IPGFTSKSMF PMLWKASGLN IDQLVAKLID ISLDS 

« Hide

References

[1]"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus."
Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W.
PLoS Genet. 3:2515-2528(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MIT 9515.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000552 Genomic DNA. Translation: ABM72674.1.
RefSeqYP_001011781.1. NC_008817.1.

3D structure databases

ProteinModelPortalA2BY13.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING167542.P9515_14671.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM72674; ABM72674; P9515_14671.
GeneID4720114.
KEGGpmc:P9515_14671.
PATRIC23016588. VBIProMar113831_1509.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG64BQ73.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycPMAR167542:GI3N-1508-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_PROM5
AccessionPrimary (citable) accession number: A2BY13
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 20, 2007
Last modified: February 19, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways