A2BWR1 (ISPE_PROM5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	P9515_10151

Organism

Prochlorococcus marinus (strain MIT 9515) [Complete proteome] [HAMAP]

Taxonomic identifier

167542 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000007872
Regions
	Nucleotide binding	101 – 111	11	ATP Potential
Sites
	Active site	16	1	By similarity
	Active site	143	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2BWR1 [UniParc]. Last modified February 20, 2007. Version 1. Checksum: 09BFA60BD79E8E56	FASTA	312	34,941
	10 20 30 40 50 60 MTKLSTTKIC VKSPAKINLH LEIIGKREDG YHELAMIMQN IDLFDYIEFE NNQIGDIELK 70 80 90 100 110 120 SNNKDLSLKD DNLIIKAANY VKDISKKKDL GANIFLKKNI PIGAGLAGGS SNAAATIIGL 130 140 150 160 170 180 NKLWDLDLDN ETMLSLSSKL GSDVPFFING GCQFCFGRGE ILENYNSKFD YGVILLKNPN 190 200 210 220 230 240 ISISTADTYK KYSKEYCSNF LTESEKTNDI RNDLRINGFN DLNSSNQIIK VKNDLQIIVE 250 260 270 280 290 300 KENDSVKKAL YLLSNLQNCL SFSMSGSGPT CFALFKDINK ANEVFEQNHK MFNNNGFEAW 310 VCKLINSGIT FL

References

[1]

"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus." Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W. PLoS Genet. 3:2515-2528(2007) [PubMed: 18159947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MIT 9515.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000552 Genomic DNA. Translation: ABM72222.1.
RefSeq	YP_001011329.1. NC_008817.1.
3D structure databases
ProteinModelPortal	A2BWR1.
ModBase	Search...
Protein-protein interaction databases
STRING	A2BWR1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4719163.
GenomeReviews	Gene locus P9515_10151 in contig CP000552_GR.
KEGG	pmc:P9515_10151.
PATRIC	23015634. VBIProMar113831_1041.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	QLCFGRG.
PhylomeDB	A2BWR1.
ProtClustDB	PRK02534.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_PROM5

Accession

Primary (citable) accession number: A2BWR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 20, 2007
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program