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A2BUL8

- SYI_PROM5

UniProt

A2BUL8 - SYI_PROM5

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Protein

Isoleucine--tRNA ligase

Gene
ileS, P9515_02701
Organism
Prochlorococcus marinus (strain MIT 9515)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei582 – 5821Aminoacyl-adenylate By similarity
Binding sitei626 – 6261ATP By similarity
Metal bindingi936 – 9361Zinc By similarity
Metal bindingi939 – 9391Zinc By similarity
Metal bindingi956 – 9561Zinc By similarity
Metal bindingi959 – 9591Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPMAR167542:GI3N-274-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:P9515_02701
OrganismiProchlorococcus marinus (strain MIT 9515)
Taxonomic identifieri167542 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000001589: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 965965Isoleucine--tRNA ligaseUniRule annotationPRO_1000022099Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi167542.P9515_02701.

Structurei

3D structure databases

ProteinModelPortaliA2BUL8.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi68 – 7811"HIGH" regionUniRule annotationAdd
BLAST
Motifi623 – 6275"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2BUL8-1 [UniParc]FASTAAdd to Basket

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MKPKNNKDKK SKFSYKETLN LLKTDFSMRA NSVSREPQIQ EFWLKNKIDL    50
ELGSSNLGEK FTLHDGPPYA NGSLHMGHAL NKVLKDIINK YKTLKGFKVH 100
FVPGWDCHGL PIELKVLQSL KSHERKDLDS LGLRKKATDY AKIQIKNQLE 150
GFKRWGIWGD WDNPYLTLKN NYESAQIGVF GKMFLNGYIY RGLKPVHWSP 200
SSRTALAEAE LEYPEEHYSS SVYVSLNISN LSDEILLKLE EKDLKNKLLS 250
NLNKLFIAIW TTTPWTIPGN EAVAINPRIN YVFAEDQNGD IFLFAKDLIS 300
EISEKLEREF NTLIDVKGSM LTGIEYKHPT KNKFCNIVIG GDYITTESGT 350
GIVHTAPGHG MDDFNVGKKY HLPITCIVDE KGNLNNHAEK FCGLNVLKDA 400
NDLIIEDLKK NNLLLLKEKY KHRYPYDWRT KKPTIFRATE QWFASVEGFR 450
SSALKAIEDV EWMPKTGKKR IYSMVVGRGD WCISRQRSWG VPIPVFYEKK 500
GKEILLNSET INHIQKLFNE HGADIWWDWD EKDLLPKQYK EESDRWIKGL 550
DTMDVWFDSG SSWAAVCEQR EELAYPADLY LEGSDQHRGW FQSSLLTSVA 600
VNNKPPYKKV LTHGFALDEN GRKMSKSLGN VVDPNIIING GSNQKIQPAY 650
GADVLRLWVS SVDYSVDVPI GSNILKQLSD VYRKVRNTAR YLLGNIHDYD 700
PIDEEIDIDQ LPILDQWMLN RLVDVSDQIT IAYENYEFSK FFQILQSFCV 750
VDLSNFYLDI AKDRLYVSAK SSIRRRTCQF VMSKIVENLA VLISPVLCHM 800
AEDIWQNIPY STNEKSVFER GWPNLPKSWI NPELNERISN LRKLRVEINK 850
AIEGCRTQQI IGAALETEVN YLPENEIIKN SLSWLEKFGN KEVDLYSDWL 900
IVSKFNVVNN LFEDYLIIDD NELGKIQILK AKGQKCDRCW HYQSNTVMGI 950
EDTKLCKRCA NIITS 965
Length:965
Mass (Da):111,215
Last modified:February 20, 2007 - v1
Checksum:i4280F03BCF3C776D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000552 Genomic DNA. Translation: ABM71479.1.
RefSeqiWP_011819591.1. NC_008817.1.
YP_001010586.1. NC_008817.1.

Genome annotation databases

EnsemblBacteriaiABM71479; ABM71479; P9515_02701.
GeneIDi4720407.
KEGGipmc:P9515_02701.
PATRICi23014062. VBIProMar113831_0273.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000552 Genomic DNA. Translation: ABM71479.1 .
RefSeqi WP_011819591.1. NC_008817.1.
YP_001010586.1. NC_008817.1.

3D structure databases

ProteinModelPortali A2BUL8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 167542.P9515_02701.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABM71479 ; ABM71479 ; P9515_02701 .
GeneIDi 4720407.
KEGGi pmc:P9515_02701.
PATRICi 23014062. VBIProMar113831_0273.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PMAR167542:GI3N-274-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MIT 9515.

Entry informationi

Entry nameiSYI_PROM5
AccessioniPrimary (citable) accession number: A2BUL8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 20, 2007
Last modified: September 3, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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