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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Prochlorococcus marinus (strain MIT 9515)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei582Aminoacyl-adenylateUniRule annotation1
Binding sitei626ATPUniRule annotation1
Metal bindingi936ZincUniRule annotation1
Metal bindingi939ZincUniRule annotation1
Metal bindingi956ZincUniRule annotation1
Metal bindingi959ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPMAR167542:G1G7Y-265-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:P9515_02701
OrganismiProchlorococcus marinus (strain MIT 9515)
Taxonomic identifieri167542 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesProchloraceaeProchlorococcus
Proteomesi
  • UP000001589 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000220991 – 965Isoleucine--tRNA ligaseAdd BLAST965

Proteomic databases

PRIDEiA2BUL8

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi167542.P9515_02701

Structurei

3D structure databases

ProteinModelPortaliA2BUL8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi68 – 78"HIGH" regionAdd BLAST11
Motifi623 – 627"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A2BUL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPKNNKDKK SKFSYKETLN LLKTDFSMRA NSVSREPQIQ EFWLKNKIDL
60 70 80 90 100
ELGSSNLGEK FTLHDGPPYA NGSLHMGHAL NKVLKDIINK YKTLKGFKVH
110 120 130 140 150
FVPGWDCHGL PIELKVLQSL KSHERKDLDS LGLRKKATDY AKIQIKNQLE
160 170 180 190 200
GFKRWGIWGD WDNPYLTLKN NYESAQIGVF GKMFLNGYIY RGLKPVHWSP
210 220 230 240 250
SSRTALAEAE LEYPEEHYSS SVYVSLNISN LSDEILLKLE EKDLKNKLLS
260 270 280 290 300
NLNKLFIAIW TTTPWTIPGN EAVAINPRIN YVFAEDQNGD IFLFAKDLIS
310 320 330 340 350
EISEKLEREF NTLIDVKGSM LTGIEYKHPT KNKFCNIVIG GDYITTESGT
360 370 380 390 400
GIVHTAPGHG MDDFNVGKKY HLPITCIVDE KGNLNNHAEK FCGLNVLKDA
410 420 430 440 450
NDLIIEDLKK NNLLLLKEKY KHRYPYDWRT KKPTIFRATE QWFASVEGFR
460 470 480 490 500
SSALKAIEDV EWMPKTGKKR IYSMVVGRGD WCISRQRSWG VPIPVFYEKK
510 520 530 540 550
GKEILLNSET INHIQKLFNE HGADIWWDWD EKDLLPKQYK EESDRWIKGL
560 570 580 590 600
DTMDVWFDSG SSWAAVCEQR EELAYPADLY LEGSDQHRGW FQSSLLTSVA
610 620 630 640 650
VNNKPPYKKV LTHGFALDEN GRKMSKSLGN VVDPNIIING GSNQKIQPAY
660 670 680 690 700
GADVLRLWVS SVDYSVDVPI GSNILKQLSD VYRKVRNTAR YLLGNIHDYD
710 720 730 740 750
PIDEEIDIDQ LPILDQWMLN RLVDVSDQIT IAYENYEFSK FFQILQSFCV
760 770 780 790 800
VDLSNFYLDI AKDRLYVSAK SSIRRRTCQF VMSKIVENLA VLISPVLCHM
810 820 830 840 850
AEDIWQNIPY STNEKSVFER GWPNLPKSWI NPELNERISN LRKLRVEINK
860 870 880 890 900
AIEGCRTQQI IGAALETEVN YLPENEIIKN SLSWLEKFGN KEVDLYSDWL
910 920 930 940 950
IVSKFNVVNN LFEDYLIIDD NELGKIQILK AKGQKCDRCW HYQSNTVMGI
960
EDTKLCKRCA NIITS
Length:965
Mass (Da):111,215
Last modified:February 20, 2007 - v1
Checksum:i4280F03BCF3C776D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000552 Genomic DNA Translation: ABM71479.1
RefSeqiWP_011819591.1, NC_008817.1

Genome annotation databases

EnsemblBacteriaiABM71479; ABM71479; P9515_02701
KEGGipmc:P9515_02701

Similar proteinsi

Entry informationi

Entry nameiSYI_PROM5
AccessioniPrimary (citable) accession number: A2BUL8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 20, 2007
Last modified: May 23, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health