A2BR98 (ACSF_PROMS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase
Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase
EC=1.14.13.81

Gene names

Name:	acsF
Ordered Locus Names:	A9601_10251

Organism

Prochlorococcus marinus (strain AS9601) [Complete proteome] [HAMAP]

Taxonomic identifier

146891 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity. HAMAP MF_01840
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O. HAMAP MF_01840 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O. HAMAP MF_01840 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = divinylprotochlorophyllide + NADP+ + 2 H2O. HAMAP MF_01840
Cofactor	Iron By similarity. HAMAP MF_01840
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis (light-independent). HAMAP MF_01840
Sequence similarities	Belongs to the AcsF family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: EC transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase HAMAP MF_01840		PRO_1000070549

Sequences

	Sequence		Length	Mass (Da)
	A2BR98 [UniParc]. Last modified February 20, 2007. Version 1. Checksum: 95B341EDB84E11BC	FASTA	390	45,687
	10 20 30 40 50 60 MAQSTVESKN RKDINNGKIP AKETILSPRF YTTDFEAMEN MDLSINEEEL EAICEEFRKD 70 80 90 100 110 120 YNRHHFVRNS EFEGAAEKLD PETRELFVDF LEGSCTSEFS GFLLYKELSK RIKVKNPLLA 130 140 150 160 170 180 ECFAHMARDE ARHAGFLNKS MSDFGLQLDL GFLTANKDYT YFPPRSIFYA TYLSEKIGYW 190 200 210 220 230 240 RYIAIYRHLE KNPDSKIFPL FNYFENWCQD ENRHGDFFDA LMKAQPRTVK SLSKKITIGG 250 260 270 280 290 300 STFTHPLFDY FHRFRYFLNN LPLTSKLWSR FFLLAVFATM YARDLGIKKD FYSSLGLDAR 310 320 330 340 350 360 DYDQFVINKT NETAARVFPV VMDVYDNSFY GRLDKIVENN KVLSDIANSD GNKVSKTFKK 370 380 390 LPKYLSNGYQ LLRLYLLKPL DSKDFQPSIR

References

[1]

"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus." Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W. PLoS Genet. 3:2515-2528(2007) [PubMed: 18159947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AS9601.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000551 Genomic DNA. Translation: ABM70309.1.
RefSeq	YP_001009416.1. NC_008816.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A2BR98.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4717736.
GenomeReviews	Gene locus A9601_10251 in contig CP000551_GR.
KEGG	pmb:A9601_10251.
PATRIC	22983553. VBIProMar75723_1032.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG10633.
HOGENOM	HBG284843.
OMA	HGDFFDA.
PhylomeDB	A2BR98.
ProtClustDB	PRK13654.
Enzyme and pathway databases
BioCyc	PMAR146891:A9601_10251-MONOMER.
Family and domain databases
HAMAP	MF_01840. AcsF. [Tree]
InterPro	IPR008434. AcsF. IPR012347. Ferritin-rel. IPR009078. Ferritin/RR-like. IPR003251. Rubrerythrin. [Graphical view]
Gene3D	G3DSA:1.20.1260.10. Ferritin_rel. 1 hit.
KO	K04035.
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACSF_PROMS

Accession

Primary (citable) accession number: A2BR98

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 20, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program