A2BR01 (ISPE_PROMS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	A9601_09281

Organism

Prochlorococcus marinus (strain AS9601) [Complete proteome] [HAMAP]

Taxonomic identifier

146891 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000007873
Regions
	Nucleotide binding	100 – 110	11	ATP Potential
Sites
	Active site	16	1	By similarity
	Active site	142	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2BR01 [UniParc]. Last modified February 20, 2007. Version 1. Checksum: E8459C21D48DF98E	FASTA	311	34,805
	10 20 30 40 50 60 MQDFAKKKIN IKSPAKINLH LEVIGKREDG FHELAMIMQN IDLADYLEFE INNEGLIKLE 70 80 90 100 110 120 SDCNDLSLSD DNLIVKSANL LRKKSNIDYG ANIFLRKNIP IGAGLAGGSS NAAATLIGLN 130 140 150 160 170 180 NLWDLKLDQE TLCSLASTLG SDIPFFINGG IQLCFGRGEI LEKLDSTLEY GAILLKNPNV 190 200 210 220 230 240 SVSTAETYKK YSNRFCDQYL TDREMIENIR KNLRDNGLNN LNFDNQHLSI KNDLQLVVEN 250 260 270 280 290 300 ENDSVKQALY LLSKLENCLT FSMSGSGPTC FALFKDKETA KKELTANSKL FKDKGYDSWV 310 CTFLEKGITF I

References

[1]

"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus." Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W. PLoS Genet. 3:2515-2528(2007) [PubMed: 18159947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AS9601.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000551 Genomic DNA. Translation: ABM70212.1.
RefSeq	YP_001009319.1. NC_008816.1.
3D structure databases
ProteinModelPortal	A2BR01.
ModBase	Search...
Protein-protein interaction databases
STRING	A2BR01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4717635.
GenomeReviews	Gene locus A9601_09281 in contig CP000551_GR.
KEGG	pmb:A9601_09281.
PATRIC	22983353. VBIProMar75723_0934.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	QLCFGRG.
PhylomeDB	A2BR01.
ProtClustDB	PRK02534.
Enzyme and pathway databases
BioCyc	PMAR146891:A9601_09281-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_PROMS

Accession

Primary (citable) accession number: A2BR01

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 20, 2007
Last modified:	December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program