A2BPV5 (SYE_PROMS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	A9601_05281

Organism

Prochlorococcus marinus (strain AS9601) [Complete proteome] [HAMAP]

Taxonomic identifier

146891 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_1000001935
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP MF_00022_B
	Motif	248 – 252	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	251	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2BPV5 [UniParc]. Last modified February 20, 2007. Version 1. Checksum: 9D34A58CE8DBBCAD	FASTA	476	55,285
	10 20 30 40 50 60 MEKRLRLAPS PTGLFHIGTA RTALFNWLYA QKIGGKFLIR IEDTDFLRSK SEYTKNILQG 70 80 90 100 110 120 LKWLGLKWDE EPIKQSDRIS IHKSHIKKLL ECGAAYRCFT SEDEISELRE EQKKKGLPPK 130 140 150 160 170 180 HDNRHRSLSK EEIETFISQG RTSVIRFKID EKIVIKWIDQ IRGEIKWQGK DLGGDLVLSR 190 200 210 220 230 240 RAKGYEIGDP LYNLAVVVDD NFMNITHVVR GEDHISNTAK QILIYNALNF NLPTFSHTPL 250 260 270 280 290 300 ILNSEGKKLS KRDCVTSIDE FREMGYLPEA LSNYMAFLGW SPKTADREIL SLEEISEIFD 310 320 330 340 350 360 LSEINKAGAK FSWEKLNWIN SQYIKNMESI KLVEIMRTYW DENGWEPPSQ EWANKLAILI 370 380 390 400 410 420 RDSMTLLKDS IDQSKPFFLI PTIQKEGQDF LENRESKLSL KLILNYLIEK NTIKLNKEKA 430 440 450 460 470 KEIINEISKK HNIKKGILMK SLRVAFFGSL IGPDLIQSWE LFAESKTDRT RIERCL

References

[1]

"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus." Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W. PLoS Genet. 3:2515-2528(2007) [PubMed: 18159947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AS9601.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000551 Genomic DNA. Translation: ABM69816.1.
RefSeq	YP_001008923.1. NC_008816.1.
3D structure databases
ProteinModelPortal	A2BPV5.
ModBase	Search...
Protein-protein interaction databases
STRING	A2BPV5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4717226.
GenomeReviews	Gene locus A9601_05281 in contig CP000551_GR.
KEGG	pmb:A9601_05281.
PATRIC	22982527. VBIProMar75723_0527.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	DKETAND.
PhylomeDB	A2BPV5.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	PMAR146891:A9601_05281-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_PROMS

Accession

Primary (citable) accession number: A2BPV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 20, 2007
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program