A2BP63 (ISPH_PROMS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	A9601_02861

Organism

Prochlorococcus marinus (strain AS9601) [Complete proteome] [HAMAP]

Taxonomic identifier

146891 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000021155

Sequences

	Sequence		Length	Mass (Da)
	A2BP63 [UniParc]. Last modified February 20, 2007. Version 1. Checksum: 5CE164388AE21952	FASTA	398	44,964
	10 20 30 40 50 60 MDTQAFRRSL HHSDRYNRRG FDSPTKRAQA LEEAYQSDLI SSIRDNGFTY TKGRLNIKLA 70 80 90 100 110 120 QAFGFCWGVE RAVAMAYETR RHYPNENIWI TNEIIHNPSV NDHLRKMNVK FISAKNGIKD 130 140 150 160 170 180 FSLVSNGDVV ILPAFGATVQ EMKLLHEKGC HIIDTTCPWV SKVWHTVEKH KKHVFTSIIH 190 200 210 220 230 240 GKFKHEETLA TSSFAGKYLV VLDLEEANYV SEYILGKGNR NEFMNKFSKA FSNGFDPDKD 250 260 270 280 290 300 LDRVGVANQT TMLKSETEEI GKVFERTMLK KFGPENLNSH FLAFNTICDA TEERQDAMFS 310 320 330 340 350 360 LVDEDLDILV VIGGFNSSNT THLQEIAITK NISSFHIDTP ERISVKENSI FHKPLGSELE 370 380 390 LKKNFLPSGK INVGITSGAS TPDKVVADVI EKLIDIAS

References

[1]

"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus." Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W. PLoS Genet. 3:2515-2528(2007) [PubMed: 18159947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AS9601.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000551 Genomic DNA. Translation: ABM69574.1.
RefSeq	YP_001008681.1. NC_008816.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A2BP63.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4716972.
GenomeReviews	Gene locus A9601_02861 in contig CP000551_GR.
KEGG	pmb:A9601_02861.
PATRIC	22982025. VBIProMar75723_0287.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0761.
HOGENOM	HBG286221.
OMA	VQEMQLL.
PhylomeDB	A2BP63.
ProtClustDB	PRK13371.
Enzyme and pathway databases
BioCyc	PMAR146891:A9601_02861-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_PROMS

Accession

Primary (citable) accession number: A2BP63

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 20, 2007
Last modified:	December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program