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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Prochlorococcus marinus (strain AS9601)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei582Aminoacyl-adenylateUniRule annotation1
Binding sitei626ATPUniRule annotation1
Metal bindingi936ZincUniRule annotation1
Metal bindingi939ZincUniRule annotation1
Metal bindingi956ZincUniRule annotation1
Metal bindingi959ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPMAR146891:G1G7X-254-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:A9601_02591
OrganismiProchlorococcus marinus (strain AS9601)
Taxonomic identifieri146891 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesProchloraceaeProchlorococcus
Proteomesi
  • UP000002590 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000221011 – 968Isoleucine--tRNA ligaseAdd BLAST968

Proteomic databases

PRIDEiA2BP36

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi146891.A9601_02591

Structurei

3D structure databases

ProteinModelPortaliA2BP36
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi68 – 78"HIGH" regionAdd BLAST11
Motifi623 – 627"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A2BP36-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSQNSKEQK SDFSYKETLN LLKTDFSMRA NSVVREPEIQ NFWAKNNIDF
60 70 80 90 100
KLGSNNSGEI FTLHDGPPYA NGALHMGHAL NKVLKDIINK YKTLRGFRVH
110 120 130 140 150
YVPGWDCHGL PIELKVLQNL KSDERKNLDT LNLRKKATDY AHIQINNQME
160 170 180 190 200
GFKRWGIWGD WDNPYLTLKK SYESAQIGVF GKMFLNGYIY RGLKPVHWSP
210 220 230 240 250
SSRTALAEAE LEYPDEHYSK SIYISLKITK ISEEILLNFV QENLNIKREF
260 270 280 290 300
FENNSFITIW TTTPWTIPAN EAVAVNPKIK YVFAIDEEKR IYLFAKDLST
310 320 330 340 350
EISNKFNKDL KVLLEVKGSQ LENIEYQHPS KNKNCRIVIG GDYITTESGT
360 370 380 390 400
GIVHTAPGHG VDDFNVGQKY DLPITCVVDE KGNLNEYSGQ FQGSNVLKDA
410 420 430 440 450
NDLIIEYLKG NNLLLLQENY KHRYPYDWRT KKPTIFRATE QWFASVNGFR
460 470 480 490 500
SSALQAIEDV EWMPATGKKR IYSMVVGRGD WCISRQRSWG VPIPVFYKKN
510 520 530 540 550
GNDILLNSEI INHIQELFSE HGADIWWDWD VKNLLPEKYA RESDLWKKGT
560 570 580 590 600
DTMDVWFDSG SSWAAVCELR SELKYPADLY LEGSDQHRGW FQSSLLTSVA
610 620 630 640 650
VNNKPPYKKV LTHGFALDEN GRKMSKSLGN VVDPNIIING GNNKKTEPAY
660 670 680 690 700
GADVLRLWVS SVDYSVDVPI GSNILKQLAD VYRKVRNTAR YLLGNIHDYD
710 720 730 740 750
PNIDSYEIDQ LPLLDQWMLG RLVEVTDQIS NAYENYEFSK FFQILQSFCV
760 770 780 790 800
VDLSNFYLDI AKDRLYVSSK SQFRRRSCQF VMSKVVENLA VLISPVLCHM
810 820 830 840 850
AEDIWQNIPY LTKEKSVFQR GWPIFAQSWK NQILNEHIAN LRNLRVEINK
860 870 880 890 900
AIEGCRNKQI IGAALETEVN YLPEDKVVKD SLIWLQEFGS QDVDLFRDWL
910 920 930 940 950
IVSNFQVVSD LVENSLIIDN NALGKIQIIK AQGQKCDRCW HYQKETFNGI
960
QNTKLCKRCS NIINLEFT
Length:968
Mass (Da):111,683
Last modified:February 20, 2007 - v1
Checksum:i98CBB89794DD661B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000551 Genomic DNA Translation: ABM69547.1
RefSeqiWP_011817731.1, NC_008816.1

Genome annotation databases

EnsemblBacteriaiABM69547; ABM69547; A9601_02591
GeneIDi4716944
KEGGipmb:A9601_02591

Similar proteinsi

Entry informationi

Entry nameiSYI_PROMS
AccessioniPrimary (citable) accession number: A2BP36
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 20, 2007
Last modified: May 23, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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