SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A2BP36

- SYI_PROMS

UniProt

A2BP36 - SYI_PROMS

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Isoleucine--tRNA ligase
Gene
ileS, A9601_02591
Organism
Prochlorococcus marinus (strain AS9601)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei582 – 5821Aminoacyl-adenylate By similarity
Binding sitei626 – 6261ATP By similarity
Metal bindingi936 – 9361Zinc By similarity
Metal bindingi939 – 9391Zinc By similarity
Metal bindingi956 – 9561Zinc By similarity
Metal bindingi959 – 9591Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPMAR146891:GH90-263-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:A9601_02591
OrganismiProchlorococcus marinus (strain AS9601)
Taxonomic identifieri146891 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000002590: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 968968Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022101Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi146891.A9601_02591.

Structurei

3D structure databases

ProteinModelPortaliA2BP36.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi68 – 7811"HIGH" regionUniRule annotation
Add
BLAST
Motifi623 – 6275"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2BP36-1 [UniParc]FASTAAdd to Basket

« Hide

MKSQNSKEQK SDFSYKETLN LLKTDFSMRA NSVVREPEIQ NFWAKNNIDF    50
KLGSNNSGEI FTLHDGPPYA NGALHMGHAL NKVLKDIINK YKTLRGFRVH 100
YVPGWDCHGL PIELKVLQNL KSDERKNLDT LNLRKKATDY AHIQINNQME 150
GFKRWGIWGD WDNPYLTLKK SYESAQIGVF GKMFLNGYIY RGLKPVHWSP 200
SSRTALAEAE LEYPDEHYSK SIYISLKITK ISEEILLNFV QENLNIKREF 250
FENNSFITIW TTTPWTIPAN EAVAVNPKIK YVFAIDEEKR IYLFAKDLST 300
EISNKFNKDL KVLLEVKGSQ LENIEYQHPS KNKNCRIVIG GDYITTESGT 350
GIVHTAPGHG VDDFNVGQKY DLPITCVVDE KGNLNEYSGQ FQGSNVLKDA 400
NDLIIEYLKG NNLLLLQENY KHRYPYDWRT KKPTIFRATE QWFASVNGFR 450
SSALQAIEDV EWMPATGKKR IYSMVVGRGD WCISRQRSWG VPIPVFYKKN 500
GNDILLNSEI INHIQELFSE HGADIWWDWD VKNLLPEKYA RESDLWKKGT 550
DTMDVWFDSG SSWAAVCELR SELKYPADLY LEGSDQHRGW FQSSLLTSVA 600
VNNKPPYKKV LTHGFALDEN GRKMSKSLGN VVDPNIIING GNNKKTEPAY 650
GADVLRLWVS SVDYSVDVPI GSNILKQLAD VYRKVRNTAR YLLGNIHDYD 700
PNIDSYEIDQ LPLLDQWMLG RLVEVTDQIS NAYENYEFSK FFQILQSFCV 750
VDLSNFYLDI AKDRLYVSSK SQFRRRSCQF VMSKVVENLA VLISPVLCHM 800
AEDIWQNIPY LTKEKSVFQR GWPIFAQSWK NQILNEHIAN LRNLRVEINK 850
AIEGCRNKQI IGAALETEVN YLPEDKVVKD SLIWLQEFGS QDVDLFRDWL 900
IVSNFQVVSD LVENSLIIDN NALGKIQIIK AQGQKCDRCW HYQKETFNGI 950
QNTKLCKRCS NIINLEFT 968
Length:968
Mass (Da):111,683
Last modified:February 20, 2007 - v1
Checksum:i98CBB89794DD661B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000551 Genomic DNA. Translation: ABM69547.1.
RefSeqiWP_011817731.1. NC_008816.1.
YP_001008654.1. NC_008816.1.

Genome annotation databases

EnsemblBacteriaiABM69547; ABM69547; A9601_02591.
GeneIDi4716944.
KEGGipmb:A9601_02591.
PATRICi22981967. VBIProMar75723_0259.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000551 Genomic DNA. Translation: ABM69547.1 .
RefSeqi WP_011817731.1. NC_008816.1.
YP_001008654.1. NC_008816.1.

3D structure databases

ProteinModelPortali A2BP36.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 146891.A9601_02591.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABM69547 ; ABM69547 ; A9601_02591 .
GeneIDi 4716944.
KEGGi pmb:A9601_02591.
PATRICi 22981967. VBIProMar75723_0259.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PMAR146891:GH90-263-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AS9601.

Entry informationi

Entry nameiSYI_PROMS
AccessioniPrimary (citable) accession number: A2BP36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 20, 2007
Last modified: September 3, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi