A2BN21 (RTCA_HYPBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA 3'-terminal phosphate cyclase
Short name=RNA cyclase
Short name=RNA-3'-phosphate cyclase
EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	Hbut_1561

Organism

Hyperthermus butylicus (strain DSM 5456 / JCM 9403)

Taxonomic identifier

415426 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Pyrodictiaceae › Hyperthermus

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP MF_00200
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential HAMAP MF_00200.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_0000325189
Regions
	Nucleotide binding	291 – 294	4	ATP By similarity
Sites
	Active site	317	1	Tele-AMP-histidine intermediate By similarity
	Binding site	100	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2BN21 [UniParc]. Last modified February 20, 2007. Version 1. Checksum: 3E819662376B17DB	FASTA	359	38,709
	10 20 30 40 50 60 MIVIDGSIGE GGGQILRTTL ALAALLGKPV RIVNIRAKRP RPGLQRQHLT SVKAVAELAS 70 80 90 100 110 120 ARVEGLELGS TTLVFIPRGL RSGRFYFNIG TAGSITLVLQ ALLPVTAFAP GPVEVEIVGG 130 140 150 160 170 180 TDVPWSPPID YVRFVLRKLL AMFGFEFEII VKRRGHYPRG GGRVVLRVTQ PPHVLKPVKL 190 200 210 220 230 240 EERGKVLRVE GLSHAVRLPR HVAERQARSA EAVLRSKLPG VPISIDLEWY EPSRDPHLGP 250 260 270 280 290 300 GSGVVVWAVA EHSVLGSDSL GAKGKPAEAV GREAAEKLLE DLATGTALDR HASDMLIPYA 310 320 330 340 350 ALACGESILG GARLTMHAWT NIEVVKMLVP GAEMEFIEGG KLNEKFKLRV KGICYKPSS

References

[1]

"The genome of Hyperthermus butylicus: a sulfur-reducing, peptide fermenting, neutrophilic Crenarchaeote growing up to 108 degrees C." Bruegger K., Chen L., Stark M., Zibat A., Redder P., Ruepp A., Awayez M., She Q., Garrett R.A., Klenk H.-P. Archaea 2:127-135(2007) [PubMed: 17350933] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 5456 / JCM 9403.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000493 Genomic DNA. Translation: ABM81382.1.
RefSeq	YP_001013727.1. NC_008818.1.
3D structure databases
ProteinModelPortal	A2BN21.
ModBase	Search...
Protein-protein interaction databases
STRING	A2BN21.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4781412.
GenomeReviews	Gene locus Hbut_1561 in contig CP000493_GR.
KEGG	hbu:Hbut_1561.
Phylogenomic databases
eggNOG	arNOG04553.
HOGENOM	HBG683539.
OMA	IHIENIR.
PhylomeDB	A2BN21.
ProtClustDB	PRK04204.
Enzyme and pathway databases
BioCyc	HBUT415426:HBUT_1561-MONOMER.
Family and domain databases
HAMAP	MF_00200. RTC. [Tree]
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR023797. RNA3'_phos_cyclase_dom. IPR000228. RNA3'_term_phos_cyc. IPR017770. RNA3'_term_phos_cyc_type_1. IPR020719. RNA3'_term_phos_cycl-like_CS. IPR013796. RNA3'_term_phos_cycl_insert. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.30.360.20. G3DSA:3.30.360.20. 1 hit. G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 2 hits.
KO	K01974.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_HYPBU

Accession

Primary (citable) accession number: A2BN21

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	February 20, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program