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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Hyperthermus butylicus (strain DSM 5456 / JCM 9403)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.UniRule annotation

Catalytic activityi

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei100 – 1001ATPUniRule annotation
Active sitei317 – 3171Tele-AMP-histidine intermediateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi291 – 2944ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA-3'-phosphate cyclase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHBUT415426:GC56-1561-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclaseUniRule annotation (EC:6.5.1.4UniRule annotation)
Short name:
RNA cyclaseUniRule annotation
Short name:
RNA-3'-phosphate cyclaseUniRule annotation
Gene namesi
Name:rtcAUniRule annotation
Ordered Locus Names:Hbut_1561
OrganismiHyperthermus butylicus (strain DSM 5456 / JCM 9403)
Taxonomic identifieri415426 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesPyrodictiaceaeHyperthermus
ProteomesiUP000002593 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359RNA 3'-terminal phosphate cyclasePRO_0000325189Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi415426.Hbut_1561.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0430.
HOGENOMiHOG000015264.
KOiK01974.
OMAiDMAVVYM.

Family and domain databases

Gene3Di3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPiMF_00200. RTC.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
PIRSFiPIRSF005378. RNA3'_term_phos_cycl_euk. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEiPS01287. RTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2BN21-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVIDGSIGE GGGQILRTTL ALAALLGKPV RIVNIRAKRP RPGLQRQHLT
60 70 80 90 100
SVKAVAELAS ARVEGLELGS TTLVFIPRGL RSGRFYFNIG TAGSITLVLQ
110 120 130 140 150
ALLPVTAFAP GPVEVEIVGG TDVPWSPPID YVRFVLRKLL AMFGFEFEII
160 170 180 190 200
VKRRGHYPRG GGRVVLRVTQ PPHVLKPVKL EERGKVLRVE GLSHAVRLPR
210 220 230 240 250
HVAERQARSA EAVLRSKLPG VPISIDLEWY EPSRDPHLGP GSGVVVWAVA
260 270 280 290 300
EHSVLGSDSL GAKGKPAEAV GREAAEKLLE DLATGTALDR HASDMLIPYA
310 320 330 340 350
ALACGESILG GARLTMHAWT NIEVVKMLVP GAEMEFIEGG KLNEKFKLRV

KGICYKPSS
Length:359
Mass (Da):38,709
Last modified:February 20, 2007 - v1
Checksum:i3E819662376B17DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000493 Genomic DNA. Translation: ABM81382.1.
RefSeqiYP_001013727.1. NC_008818.1.

Genome annotation databases

EnsemblBacteriaiABM81382; ABM81382; Hbut_1561.
GeneIDi4781412.
KEGGihbu:Hbut_1561.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000493 Genomic DNA. Translation: ABM81382.1.
RefSeqiYP_001013727.1. NC_008818.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi415426.Hbut_1561.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABM81382; ABM81382; Hbut_1561.
GeneIDi4781412.
KEGGihbu:Hbut_1561.

Phylogenomic databases

eggNOGiCOG0430.
HOGENOMiHOG000015264.
KOiK01974.
OMAiDMAVVYM.

Enzyme and pathway databases

BioCyciHBUT415426:GC56-1561-MONOMER.

Family and domain databases

Gene3Di3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPiMF_00200. RTC.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
PIRSFiPIRSF005378. RNA3'_term_phos_cycl_euk. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEiPS01287. RTC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of Hyperthermus butylicus: a sulfur-reducing, peptide fermenting, neutrophilic Crenarchaeote growing up to 108 degrees C."
    Bruegger K., Chen L., Stark M., Zibat A., Redder P., Ruepp A., Awayez M., She Q., Garrett R.A., Klenk H.-P.
    Archaea 2:127-135(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 5456 / JCM 9403.

Entry informationi

Entry nameiRTCA_HYPBU
AccessioniPrimary (citable) accession number: A2BN21
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: February 20, 2007
Last modified: April 1, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.