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Protein

Flap endonuclease 1

Gene

fen

Organism
Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA (By similarity).By similarity

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi30 – 301Magnesium 1UniRule annotation
Metal bindingi83 – 831Magnesium 1UniRule annotation
Metal bindingi155 – 1551Magnesium 1UniRule annotation
Metal bindingi157 – 1571Magnesium 1UniRule annotation
Metal bindingi176 – 1761Magnesium 2UniRule annotation
Metal bindingi178 – 1781Magnesium 2UniRule annotation
Metal bindingi239 – 2391Magnesium 2UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciHBUT415426:GC56-1366-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flap endonuclease 1UniRule annotation (EC:3.1.-.-UniRule annotation)
Short name:
FEN-1UniRule annotation
Alternative name(s):
Flap structure-specific endonuclease 1UniRule annotation
Gene namesi
Name:fenUniRule annotation
Ordered Locus Names:Hbut_1366
OrganismiHyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)
Taxonomic identifieri415426 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesPyrodictiaceaeHyperthermus
Proteomesi
  • UP000002593 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 350350Flap endonuclease 1PRO_1000061318Add
BLAST

Interactioni

Subunit structurei

Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity.UniRule annotation

Protein-protein interaction databases

STRINGi415426.Hbut_1366.

Structurei

3D structure databases

ProteinModelPortaliA2BMI0.
SMRiA2BMI0. Positions 2-334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 101101N-domainAdd
BLAST
Regioni119 – 261143I-domainAdd
BLAST
Regioni340 – 3489Interaction with PCNAUniRule annotation

Sequence similaritiesi

Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04050. Archaea.
COG0258. LUCA.
HOGENOMiHOG000193852.
KOiK04799.
OMAiGSQDYDS.

Family and domain databases

CDDicd09867. PIN_FEN1. 1 hit.
Gene3Di3.40.50.1010. 1 hit.
HAMAPiMF_00614. Fen. 1 hit.
InterProiIPR020045. 5-3_exonuclease_C.
IPR023426. Flap_endonuc.
IPR019973. Flap_structure-sp_endonuc_arc.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR006085. XPG_DNA_repair_N.
[Graphical view]
PANTHERiPTHR11081. PTHR11081. 1 hit.
PfamiPF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
[Graphical view]
PRINTSiPR00853. XPGRADSUPER.
SMARTiSM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR03674. fen_arch. 1 hit.

Sequencei

Sequence statusi: Complete.

A2BMI0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVNIREVIP PEAIQEIDLA HLKYKVVAID AYNALYQFLT AIRQPDGTPL
60 70 80 90 100
MDSKGRITSH LSGLFYRTIN LMEHGIKIVY VFDGKPPEMK YLEIERRKRV
110 120 130 140 150
KSEAVKKYEE AVKKGDTKAA RRYAQMAARL TDEMVEDAKK LLDAMGVPWV
160 170 180 190 200
QAPAEGEAQA AFMARRGDAW AAASQDYDSL LFGSPRLVRN LAITGRRKLP
210 220 230 240 250
RKDVYVEIKP ELIELDKLLK ALGITREQLV ALGILIGTDY NPDGVKGIGP
260 270 280 290 300
KTALKMVKAH RDPVKLLQGL PRHEFPTDPL KIYEYFLNPP VTTDYKLEWR
310 320 330 340 350
EPDEKKVFEI LVEGHDFSPE RVRNALERLK KAYREHFRGQ QMGLDAWLRR
Length:350
Mass (Da):40,038
Last modified:February 20, 2007 - v1
Checksum:i4EEA44065E2C49AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000493 Genomic DNA. Translation: ABM81191.1.
RefSeqiWP_011822509.1. NC_008818.1.

Genome annotation databases

EnsemblBacteriaiABM81191; ABM81191; Hbut_1366.
GeneIDi4782618.
KEGGihbu:Hbut_1366.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000493 Genomic DNA. Translation: ABM81191.1.
RefSeqiWP_011822509.1. NC_008818.1.

3D structure databases

ProteinModelPortaliA2BMI0.
SMRiA2BMI0. Positions 2-334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi415426.Hbut_1366.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABM81191; ABM81191; Hbut_1366.
GeneIDi4782618.
KEGGihbu:Hbut_1366.

Phylogenomic databases

eggNOGiarCOG04050. Archaea.
COG0258. LUCA.
HOGENOMiHOG000193852.
KOiK04799.
OMAiGSQDYDS.

Enzyme and pathway databases

BioCyciHBUT415426:GC56-1366-MONOMER.

Family and domain databases

CDDicd09867. PIN_FEN1. 1 hit.
Gene3Di3.40.50.1010. 1 hit.
HAMAPiMF_00614. Fen. 1 hit.
InterProiIPR020045. 5-3_exonuclease_C.
IPR023426. Flap_endonuc.
IPR019973. Flap_structure-sp_endonuc_arc.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR006085. XPG_DNA_repair_N.
[Graphical view]
PANTHERiPTHR11081. PTHR11081. 1 hit.
PfamiPF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
[Graphical view]
PRINTSiPR00853. XPGRADSUPER.
SMARTiSM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR03674. fen_arch. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFEN_HYPBU
AccessioniPrimary (citable) accession number: A2BMI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 20, 2007
Last modified: September 7, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.