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Protein

Flap endonuclease 1

Gene

fen

Organism
Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA (By similarity).By similarity

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi30Magnesium 1UniRule annotation1
Metal bindingi83Magnesium 1UniRule annotation1
Metal bindingi155Magnesium 1UniRule annotation1
Metal bindingi157Magnesium 1UniRule annotation1
Metal bindingi176Magnesium 2UniRule annotation1
Metal bindingi178Magnesium 2UniRule annotation1
Metal bindingi239Magnesium 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Exonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciHBUT415426:GC56-1366-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flap endonuclease 1UniRule annotation (EC:3.1.-.-UniRule annotation)
Short name:
FEN-1UniRule annotation
Alternative name(s):
Flap structure-specific endonuclease 1UniRule annotation
Gene namesi
Name:fenUniRule annotation
Ordered Locus Names:Hbut_1366
OrganismiHyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)
Taxonomic identifieri415426 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesPyrodictiaceaeHyperthermus
Proteomesi
  • UP000002593 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000613181 – 350Flap endonuclease 1Add BLAST350

Interactioni

Subunit structurei

Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity.UniRule annotation

Protein-protein interaction databases

STRINGi415426.Hbut_1366.

Structurei

3D structure databases

ProteinModelPortaliA2BMI0.
SMRiA2BMI0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 101N-domainAdd BLAST101
Regioni119 – 261I-domainAdd BLAST143
Regioni340 – 348Interaction with PCNAUniRule annotation9

Sequence similaritiesi

Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04050. Archaea.
COG0258. LUCA.
HOGENOMiHOG000193852.
KOiK04799.
OMAiGSQDYDS.
OrthoDBiPOG093Z0642.

Family and domain databases

CDDicd09867. PIN_FEN1. 1 hit.
Gene3Di3.40.50.1010. 1 hit.
HAMAPiMF_00614. Fen. 1 hit.
InterProiView protein in InterPro
IPR020045. 5-3_exonuclease_C.
IPR023426. Flap_endonuc.
IPR019973. Flap_structure-sp_endonuc_arc.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR006085. XPG_DNA_repair_N.
PANTHERiPTHR11081. PTHR11081. 1 hit.
PfamiView protein in Pfam
PF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
PRINTSiPR00853. XPGRADSUPER.
SMARTiView protein in SMART
SM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF88723. SSF88723. 1 hit.
TIGRFAMsiTIGR03674. fen_arch. 1 hit.

Sequencei

Sequence statusi: Complete.

A2BMI0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVNIREVIP PEAIQEIDLA HLKYKVVAID AYNALYQFLT AIRQPDGTPL
60 70 80 90 100
MDSKGRITSH LSGLFYRTIN LMEHGIKIVY VFDGKPPEMK YLEIERRKRV
110 120 130 140 150
KSEAVKKYEE AVKKGDTKAA RRYAQMAARL TDEMVEDAKK LLDAMGVPWV
160 170 180 190 200
QAPAEGEAQA AFMARRGDAW AAASQDYDSL LFGSPRLVRN LAITGRRKLP
210 220 230 240 250
RKDVYVEIKP ELIELDKLLK ALGITREQLV ALGILIGTDY NPDGVKGIGP
260 270 280 290 300
KTALKMVKAH RDPVKLLQGL PRHEFPTDPL KIYEYFLNPP VTTDYKLEWR
310 320 330 340 350
EPDEKKVFEI LVEGHDFSPE RVRNALERLK KAYREHFRGQ QMGLDAWLRR
Length:350
Mass (Da):40,038
Last modified:February 20, 2007 - v1
Checksum:i4EEA44065E2C49AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000493 Genomic DNA. Translation: ABM81191.1.
RefSeqiWP_011822509.1. NC_008818.1.

Genome annotation databases

EnsemblBacteriaiABM81191; ABM81191; Hbut_1366.
GeneIDi4782618.
KEGGihbu:Hbut_1366.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiFEN_HYPBU
AccessioniPrimary (citable) accession number: A2BMI0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 20, 2007
Last modified: February 15, 2017
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families