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A2BLD4 (SYW_HYPBU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase
EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Ordered Locus Names:Hbut_0947
OrganismHyperthermus butylicus (strain DSM 5456 / JCM 9403) [Reference proteome] [HAMAP]
Taxonomic identifier415426 [NCBI]
Taxonomic lineageArchaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Pyrodictiaceae › Hyperthermus ›

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 373373Tryptophan--tRNA ligase HAMAP-Rule MF_00140
PRO_1000018995

Regions

Motif79 – 879"HIGH" region HAMAP-Rule MF_00140
Motif257 – 2615"KMSKS" region HAMAP-Rule MF_00140

Sequences

Sequence LengthMass (Da)Tools
A2BLD4 [UniParc].

Last modified February 20, 2007. Version 1.
Checksum: F351ABD38649A86C

FASTA37343,646
        10         20         30         40         50         60 
MEGYRLDPWA SAIRLEYEKL FRYFGIKPFQ PLLPEVEKAF GKPHRLMRRG IIFGHRDYEK 

        70         80         90        100        110        120 
ILEAYRSGER IALVTGFMPS GKFHFGHKMV ADQIIYYQKL GFEIFVVIAD AEAYAVRRLD 

       130        140        150        160        170        180 
RRKIIEIGLY EYVANLIALG LEKNKHTHIY FQTNYETPYY RLIQMFSRRV TLEEMSAIYG 

       190        200        210        220        230        240 
DLEPSKIMAA LTQAADILHP QLDYFGGFKY VVVPVGADQD PHIRLTRDIA ARFENELGLR 

       250        260        270        280        290        300 
RPASTYHRFQ TGLDGNKMSS SRPEYTIFLT DPVDIAVRKL KRALTGGRAT VEEQRRLGGE 

       310        320        330        340        350        360 
PEKCTVYEFY LYHLIEDDTQ LRKIYEDCRG SRLLCGPDKE YAAELLAKFL EEHQRRLERA 

       370 
RDRVLEYVEP PKF

« Hide

References

[1]"The genome of Hyperthermus butylicus: a sulfur-reducing, peptide fermenting, neutrophilic Crenarchaeote growing up to 108 degrees C."
Bruegger K., Chen L., Stark M., Zibat A., Redder P., Ruepp A., Awayez M., She Q., Garrett R.A., Klenk H.-P.
Archaea 2:127-135(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 5456 / JCM 9403.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000493 Genomic DNA. Translation: ABM80795.1.
RefSeqYP_001013140.1. NC_008818.1.

3D structure databases

ProteinModelPortalA2BLD4.
ModBaseSearch...

Protein-protein interaction databases

STRING415426.Hbut_0947.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM80795; ABM80795; Hbut_0947.
GeneID4782824.
KEGGhbu:Hbut_0947.

Phylogenomic databases

eggNOGCOG0180.
HOGENOMHOG000224743.
KOK01867.
OMAKTGGRET.
ProtClustDBPRK12285.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_A. Trp_tRNA_synth_A.
InterProIPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR020653. Tryptophan-tRNA-ligase_arc.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_HYPBU
AccessionPrimary (citable) accession number: A2BLD4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 20, 2007
Last modified: May 1, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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