A2BLC6 (G3P_HYPBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase
Short name=GAPDH
EC=1.2.1.59

Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase

Gene names

Name:	gap
Ordered Locus Names:	Hbut_0939

Organism

Hyperthermus butylicus (strain DSM 5456 / JCM 9403)

Taxonomic identifier

415426 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Pyrodictiaceae › Hyperthermus

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H. HAMAP MF_00559
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. HAMAP MF_00559
Subunit structure	Homotetramer By similarity. HAMAP MF_00559
Subcellular location	Cytoplasm By similarity HAMAP MF_00559.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Glyceraldehyde-3-phosphate dehydrogenase HAMAP MF_00559		PRO_0000300970
Regions
	Nucleotide binding	13 – 14	2	NAD By similarity
	Region	140 – 142	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	195 – 196	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	141	1	Nucleophile By similarity
	Binding site	111	1	NAD; via amide nitrogen By similarity
	Binding site	169	1	NAD By similarity
	Binding site	302	1	NAD; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2BLC6 [UniParc]. Last modified February 20, 2007. Version 1. Checksum: DFAFF32D589DCBFF	FASTA	343	37,560
	10 20 30 40 50 60 MARVRVGVNG FGTIGKRVAE AIMLQPDMEL VGVTKTKPDY TAKYAVSRGI PVYVPKESLE 70 80 90 100 110 120 EFQAKGIEPA GTIEELLEKV DVIVDATPGG TGRKYKPLYE KAGVKMIFQG GEKADIAELS 130 140 150 160 170 180 FSTLCNYEQA LGKTSLRVVS CNTTGLLRAI CSISRLAPVR RVRATIVRRA ADPKEIKRGP 190 200 210 220 230 240 VNAIKPDPVK TPSHHALDVK TVLPDLDIVT MAVVVPTTLM HVHIVYAELE KPVTREDVVA 250 260 270 280 290 300 TFESTPRILL ADASYGLAST AELVEYARDL GRKRYDIPEL IVWLDSIAVN GVEVMWMQAV 310 320 330 340 HQEAIVVPEN IDAIRAVAEL AKTAEETIRI TDERLGLLKG RIP

References

[1]

"The genome of Hyperthermus butylicus: a sulfur-reducing, peptide fermenting, neutrophilic Crenarchaeote growing up to 108 degrees C." Bruegger K., Chen L., Stark M., Zibat A., Redder P., Ruepp A., Awayez M., She Q., Garrett R.A., Klenk H.-P. Archaea 2:127-135(2007) [PubMed: 17350933] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 5456 / JCM 9403.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000493 Genomic DNA. Translation: ABM80787.1.
RefSeq	YP_001013132.1. NC_008818.1.
3D structure databases
ProteinModelPortal	A2BLC6.
SMR	A2BLC6. Positions 3-339.
ModBase	Search...
Protein-protein interaction databases
STRING	A2BLC6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4782951.
GenomeReviews	Gene locus Hbut_0939 in contig CP000493_GR.
KEGG	hbu:Hbut_0939.
Phylogenomic databases
eggNOG	arNOG04694.
HOGENOM	HBG392099.
OMA	VPSHHGP.
ProtClustDB	PRK04207.
Enzyme and pathway databases
BioCyc	HBUT415426:HBUT_0939-MONOMER.
Family and domain databases
HAMAP	MF_00559. G3P_dehdrog_arch. [Tree]
InterPro	IPR020831. GlycerAld/Erythrose_P_DH. IPR020830. GlycerAld_3-P_DH_AS. IPR020829. GlycerAld_3-P_DH_cat. IPR020828. GlycerAld_3-P_DH_NAD(P)-bd. IPR006436. Glyceraldehyde-3-P_DH_2_arc. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00150.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
SMART	SM00846. Gp_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01546. GAPDH-II_archae. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P_HYPBU

Accession

Primary (citable) accession number: A2BLC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	February 20, 2007
Last modified:	November 16, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program