A2BK91 (SYE_HYPBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	Hbut_0542

Organism

Hyperthermus butylicus (strain DSM 5456 / JCM 9403) [Reference proteome] [HAMAP]

Taxonomic identifier

415426 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Pyrodictiaceae › Hyperthermus › Hyperthermus butylicus

Protein attributes

Sequence length	578 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 578	578	Glutamate--tRNA ligase HAMAP-Rule MF_00022		PRO_0000367801
Regions
	Motif	97 – 107	11	"HIGH" region HAMAP-Rule MF_00022

Sequences

	Sequence		Length	Mass (Da)
	A2BK91 [UniParc]. Last modified February 20, 2007. Version 1. Checksum: AB7854019C4E9E29	FASTA	578	66,009
	10 20 30 40 50 60 MLNAVGHGGR AAVGPVMGKI MAERPDLRPQ AKQIVAIVRE VVEEVNKLSL EEQKRLLEEK 70 80 90 100 110 120 YSWVIEKKLQ KRREAVEKKL PPLPDAEEGR VVTRFAPNPD FVIHLGNARP ALLSYEYAVM 130 140 150 160 170 180 YRGKMILRFE DTDPRIKTPL PEAYELIRED LKWLGIRWDE EYIQSLRMHI YYDIARKLIE 190 200 210 220 230 240 VGGAYVDDRS PEEFRRYRDE GRLEDYPPRK RSVEENLELW DKMVSGAFGE GEAVLRVKTD 250 260 270 280 290 300 PRHPDPSVRD WVAFRIIDTS RYPHPLVGDR YVAWPTYNFA AAVDDKLMGV THILRAKEHM 310 320 330 340 350 360 QNTIKQQFLY KHLGWSYPHV VHFGRLKLEG FIMSKSTLKR FLDAGISRGI DDPRFATIAG 370 380 390 400 410 420 LRRRGIVPEA IKKLIMEVGV KYTDASISYD NLAAINRSII DPRAKRIMAA LSPVPVEVEG 430 440 450 460 470 480 LPWREKEFRI PFHPSGQLGE RIVKLAGPKA TIYVSQSDAV QLAKGNIVRL MEAFNIEVLG 490 500 510 520 530 540 ASPRRVRARY HSLTVDEARQ HNAPIIQWVP ATDNIAVEVL KPEGLDLVQE RGLAEPAAAQ 550 560 570 LKPGEIIQLV RIGFARVDSV ETDEKGKVRK VVLIYAHD

References

[1]

"The genome of Hyperthermus butylicus: a sulfur-reducing, peptide fermenting, neutrophilic Crenarchaeote growing up to 108 degrees C." Bruegger K., Chen L., Stark M., Zibat A., Redder P., Ruepp A., Awayez M., She Q., Garrett R.A., Klenk H.-P. Archaea 2:127-135(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 5456 / JCM 9403.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000493 Genomic DNA. Translation: ABM80402.1.
RefSeq	YP_001012747.1. NC_008818.1.
3D structure databases
ProteinModelPortal	A2BK91.
ModBase	Search...
Protein-protein interaction databases
STRING	415426.Hbut_0542.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABM80402; ABM80402; Hbut_0542.
GeneID	4782144.
KEGG	hbu:Hbut_0542.
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000073585.
KO	K01885.
OMA	MRFAPNP.
ProtClustDB	PRK04156.
Family and domain databases
Gene3D	1.10.1160.10. 1 hit. 2.40.240.10. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00022_A. Glu_tRNA_synth_A.
InterPro	IPR004526. Glu-tRNA-synth_Ib_arc/euk. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR022888. Glu_tRNA_synth_arc. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00463. gltX_arch. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_HYPBU

Accession

Primary (citable) accession number: A2BK91

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 20, 2007
Last modified:	May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program