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A2BK91 (SYE_HYPBU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:Hbut_0542
OrganismHyperthermus butylicus (strain DSM 5456 / JCM 9403) [Reference proteome] [HAMAP]
Taxonomic identifier415426 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiDesulfurococcalesPyrodictiaceaeHyperthermus

Protein attributes

Sequence length578 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 578578Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000367801

Regions

Motif97 – 10711"HIGH" region HAMAP-Rule MF_00022

Sequences

Sequence LengthMass (Da)Tools
A2BK91 [UniParc].

Last modified February 20, 2007. Version 1.
Checksum: AB7854019C4E9E29

FASTA57866,009
        10         20         30         40         50         60 
MLNAVGHGGR AAVGPVMGKI MAERPDLRPQ AKQIVAIVRE VVEEVNKLSL EEQKRLLEEK 

        70         80         90        100        110        120 
YSWVIEKKLQ KRREAVEKKL PPLPDAEEGR VVTRFAPNPD FVIHLGNARP ALLSYEYAVM 

       130        140        150        160        170        180 
YRGKMILRFE DTDPRIKTPL PEAYELIRED LKWLGIRWDE EYIQSLRMHI YYDIARKLIE 

       190        200        210        220        230        240 
VGGAYVDDRS PEEFRRYRDE GRLEDYPPRK RSVEENLELW DKMVSGAFGE GEAVLRVKTD 

       250        260        270        280        290        300 
PRHPDPSVRD WVAFRIIDTS RYPHPLVGDR YVAWPTYNFA AAVDDKLMGV THILRAKEHM 

       310        320        330        340        350        360 
QNTIKQQFLY KHLGWSYPHV VHFGRLKLEG FIMSKSTLKR FLDAGISRGI DDPRFATIAG 

       370        380        390        400        410        420 
LRRRGIVPEA IKKLIMEVGV KYTDASISYD NLAAINRSII DPRAKRIMAA LSPVPVEVEG 

       430        440        450        460        470        480 
LPWREKEFRI PFHPSGQLGE RIVKLAGPKA TIYVSQSDAV QLAKGNIVRL MEAFNIEVLG 

       490        500        510        520        530        540 
ASPRRVRARY HSLTVDEARQ HNAPIIQWVP ATDNIAVEVL KPEGLDLVQE RGLAEPAAAQ 

       550        560        570 
LKPGEIIQLV RIGFARVDSV ETDEKGKVRK VVLIYAHD 

« Hide

References

[1]"The genome of Hyperthermus butylicus: a sulfur-reducing, peptide fermenting, neutrophilic Crenarchaeote growing up to 108 degrees C."
Bruegger K., Chen L., Stark M., Zibat A., Redder P., Ruepp A., Awayez M., She Q., Garrett R.A., Klenk H.-P.
Archaea 2:127-135(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 5456 / JCM 9403.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000493 Genomic DNA. Translation: ABM80402.1.
RefSeqYP_001012747.1. NC_008818.1.

3D structure databases

ProteinModelPortalA2BK91.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING415426.Hbut_0542.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM80402; ABM80402; Hbut_0542.
GeneID4782144.
KEGGhbu:Hbut_0542.

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000073585.
KOK01885.
OMAMRFAPNP.
ProtClustDBPRK04156.

Enzyme and pathway databases

BioCycHBUT415426:GC56-542-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_A. Glu_tRNA_synth_A.
InterProIPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00463. gltX_arch. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYE_HYPBU
AccessionPrimary (citable) accession number: A2BK91
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: February 20, 2007
Last modified: February 19, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries