Reviewed, UniProtKB/Swiss-Prot A2BK57 (RIFK_HYPBU)

Names and origin

Protein names

Recommended name:
    Riboflavin kinase
      Short name=RFK
    EC=2.7.1.161
Alternative name(s):
    CTP:riboflavin 5'-phosphotransferase
    CTP-dependent riboflavin kinase
    Flavokinase

Gene names

Name:	rfk
Ordered Locus Names:	Hbut_0506

Organism

Hyperthermus butylicus (strain DSM 5456 / JCM 9403) [Complete proteome] [HAMAP]

Taxonomic identifier

415426 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Pyrodictiaceae › Hyperthermus

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity.
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	FMN biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: HAMAP riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 149	149	Riboflavin kinase HAMAP MF_01285		PRO_0000322066
Regions
	Nucleotide binding	18 – 23	6	CDP By similarity
	Nucleotide binding	118 – 121	4	CDP By similarity
Sites
	Metal binding	47	1	Magnesium By similarity
	Metal binding	49	1	Magnesium By similarity
	Binding site	105	1	FMN By similarity
	Binding site	113	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2BK57-1 [UniParc]. Last modified February 20, 2007. Version 1. Checksum: 5E3054CD6CBE2A94	FASTA	149	16,712
	10 20 30 40 50 60 MENGAVQQLQ LLGKRVKGFG VGGRYVAHPY YSGRFRELLG CTPFPGTLNF DANLDWRELA 70 80 90 100 110 120 SMCEPQVIPG TVWDGVRLGA VYVWKAKIMT RHGYVDCAVI RPLLSGHPPT VLEIVACEKL 130 140 EPILENNPDK TVIVTIACKR GDALRWRRY

References

[1]

"The genome of Hyperthermus butylicus: a sulfur-reducing, peptide fermenting, neutrophilic Crenarchaeote growing up to 108 degrees C." Bruegger K., Chen L., Stark M., Zibat A., Redder P., Ruepp A., Awayez M., She Q., Garrett R.A., Klenk H.-P. Archaea 2:127-135(2006) [PubMed: 17350933] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000493 Genomic DNA. Translation: ABM80368.1.
RefSeq	YP_001012713.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A2BK57.
Genome annotation databases
GeneID	4781463.
GenomeReviews	Gene locus Hbut_0506 in contig CP000493_GR.
KEGG	hbu:Hbut_0506.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01285. [Tree]
InterPro	IPR002834. Riboflavin_kinase_arc. [Graphical view]
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProDom	PD015839. DUF120. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

RIFK_HYPBU

Accession

Primary (citable) accession number: A2BK57

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	February 20, 2007
Last modified:	September 1, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)