Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei160Proton acceptorUniRule annotation1
Binding sitei162SubstrateUniRule annotation1
Metal bindingi186Magnesium; via carbamate groupUniRule annotation1
Metal bindingi188MagnesiumUniRule annotation1
Metal bindingi189MagnesiumUniRule annotation1
Active sitei278Proton acceptorUniRule annotation1
Binding sitei279SubstrateUniRule annotation1
Binding sitei311SubstrateUniRule annotation1
Sitei319Transition state stabilizerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationImported, OxidoreductaseUniRule annotation

Keywords - Biological processi

Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciHBUT415426:GC56-503-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCOUniRule annotation
Gene namesi
Name:rbcLUniRule annotation
Ordered Locus Names:Hbut_0503Imported
OrganismiHyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)Imported
Taxonomic identifieri415426 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesPyrodictiaceaeHyperthermus
Proteomesi
  • UP000002593 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei186N6-carboxylysineUniRule annotation1

Interactioni

Subunit structurei

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

Protein-protein interaction databases

STRINGi415426.Hbut_0503.

Structurei

3D structure databases

ProteinModelPortaliA2BK54.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 129RuBisCO_large_NInterPro annotationAdd BLAST119
Domaini139 – 435RuBisCO_largeInterPro annotationAdd BLAST297

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni364 – 366Substrate bindingUniRule annotation3
Regioni386 – 389Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04443. Archaea.
COG1850. LUCA.
HOGENOMiHOG000230831.
KOiK01601.
OMAiFTQDWAS.

Family and domain databases

CDDicd08213. RuBisCO_large_III. 1 hit.
Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3. 1 hit.
InterProiIPR033966. RuBisCO.
IPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.

Sequencei

Sequence statusi: Complete.

A2BK54-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHEHFDSIYL EFVDESYKPG KDEVIAVFRV TPAQGISIKD AAGRIAAESS
60 70 80 90 100
VGTWTTLSVK PSWFEKLKAK AYRFHDLGDG SWLVWVAYPV ELFEEGSIPN
110 120 130 140 150
FASSILGNIF GMKAIAGLRV EDVYFPPSYL ETFPGPNKGI QGVREILGIK
160 170 180 190 200
DRPILATVPK PKLGYTPEEY GRVAYEILIG GIDLVKDDEN FASQPFCRFE
210 220 230 240 250
ARLKEVMKAI DRAEKETGER KGYLANVTAP IREMEKRIKL VADYGNKFIM
260 270 280 290 300
IDFLTAGWAA LQHARELAEE YDLAIHGHRA FHAAFTRNPK HGVSMFLVAK
310 320 330 340 350
LARMAGVDHV HVGTPGVGKM DAKTREVLEH TRIVREQYYR PKEGDLFHLE
360 370 380 390 400
QPWSNIKPVF PVASGGLHPG TLPEVIRVMG KDIIMQVGGG VLGHPDGPEA
410 420 430 440
GARAVRQAVE AAMKGISLDE YAREHRELAR ALEKWGYVRP V
Length:441
Mass (Da):49,178
Last modified:February 20, 2007 - v1
Checksum:iF8A627C6D6189C16
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000493 Genomic DNA. Translation: ABM80365.1.
RefSeqiWP_011821683.1. NC_008818.1.

Genome annotation databases

EnsemblBacteriaiABM80365; ABM80365; Hbut_0503.
GeneIDi4781477.
KEGGihbu:Hbut_0503.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000493 Genomic DNA. Translation: ABM80365.1.
RefSeqiWP_011821683.1. NC_008818.1.

3D structure databases

ProteinModelPortaliA2BK54.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi415426.Hbut_0503.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABM80365; ABM80365; Hbut_0503.
GeneIDi4781477.
KEGGihbu:Hbut_0503.

Phylogenomic databases

eggNOGiarCOG04443. Archaea.
COG1850. LUCA.
HOGENOMiHOG000230831.
KOiK01601.
OMAiFTQDWAS.

Enzyme and pathway databases

BioCyciHBUT415426:GC56-503-MONOMER.

Family and domain databases

CDDicd08213. RuBisCO_large_III. 1 hit.
Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3. 1 hit.
InterProiIPR033966. RuBisCO.
IPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiA2BK54_HYPBU
AccessioniPrimary (citable) accession number: A2BK54
Entry historyi
Integrated into UniProtKB/TrEMBL: February 20, 2007
Last sequence update: February 20, 2007
Last modified: November 30, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.