A2BJY7 (NTPA_HYPBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

Hbut_0432

Organism

Hyperthermus butylicus (strain DSM 5456 / JCM 9403)

Taxonomic identifier

415426 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Pyrodictiaceae › Hyperthermus

Protein attributes

Sequence length	190 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 190	190	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_1000087378
Regions
	Region	10 – 15	6	Substrate binding By similarity
	Region	68 – 69	2	Substrate binding By similarity
Sites
	Metal binding	39	1	Magnesium or manganese By similarity
	Metal binding	68	1	Magnesium or manganese By similarity
	Binding site	146	1	Substrate By similarity
	Binding site	166	1	Substrate By similarity
	Binding site	172	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2BJY7 [UniParc]. Last modified February 20, 2007. Version 1. Checksum: E34FD1C445DE4268	FASTA	190	21,059
	10 20 30 40 50 60 MEARVILIAT TNKHKIEEIN EVLQSCGYRV EPAAASKLEV QSNRLEDVAA YAAIQAYLAL 70 80 90 100 110 120 QRPVIVEDAG LFVEALGGFP GPYSSYVFKT IGIRGLLKLL EDVENRRAYF KSVIALAHSG 130 140 150 160 170 180 GVEVFTGTVH GVIAEKPRGD RGFGYDPVFI PEGSSKTFAE METQEKNKFS HRGKAARELC 190 RWLRQYGPPR

References

[1]

"The genome of Hyperthermus butylicus: a sulfur-reducing, peptide fermenting, neutrophilic Crenarchaeote growing up to 108 degrees C." Bruegger K., Chen L., Stark M., Zibat A., Redder P., Ruepp A., Awayez M., She Q., Garrett R.A., Klenk H.-P. Archaea 2:127-135(2007) [PubMed: 17350933] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 5456 / JCM 9403.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000493 Genomic DNA. Translation: ABM80298.1.
RefSeq	YP_001012643.1. NC_008818.1.
3D structure databases
ProteinModelPortal	A2BJY7.
ModBase	Search...
Protein-protein interaction databases
STRING	A2BJY7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4782980.
GenomeReviews	Gene locus Hbut_0432 in contig CP000493_GR.
KEGG	hbu:Hbut_0432.
Phylogenomic databases
eggNOG	arNOG10472.
HOGENOM	HBG697237.
OMA	VEDSGFF.
PhylomeDB	A2BJY7.
ProtClustDB	PRK14821.
Enzyme and pathway databases
BioCyc	HBUT415426:HBUT_0432-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_HYPBU

Accession

Primary (citable) accession number: A2BJY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 20, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program