Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A2BJX6 (DNLI_HYPBU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase

EC=6.5.1.1
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]
Gene names
Name:lig
Ordered Locus Names:Hbut_0421
OrganismHyperthermus butylicus (strain DSM 5456 / JCM 9403) [Reference proteome] [HAMAP]
Taxonomic identifier415426 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiDesulfurococcalesPyrodictiaceaeHyperthermus

Protein attributes

Sequence length608 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 608608DNA ligase HAMAP-Rule MF_00407
PRO_0000365248

Sites

Active site2681N6-AMP-lysine intermediate By similarity
Binding site2661ATP By similarity
Binding site2731ATP By similarity
Binding site2881ATP By similarity
Binding site3181ATP By similarity
Binding site3581ATP By similarity
Binding site4351ATP By similarity
Binding site4411ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A2BJX6 [UniParc].

Last modified February 20, 2007. Version 1.
Checksum: 7D16CF4AEB60771E

FASTA60868,330
        10         20         30         40         50         60 
MSMPFSVLAE TFEKLERVTA RTQMLLYLVE LFKKTPPEII DKVVYFIQGK LWPDWKGLPE 

        70         80         90        100        110        120 
LGIGEKMLIK AASIALHVSE KQIEQLVKKL GDTGKAIEMI KREKQQKQKA VGLLAFMQKP 

       130        140        150        160        170        180 
SGGESTLTVE KVYDTLARIA LVQGEGSRDI KLKLLAGLLA EASPREAKYI ARFVEGRLRL 

       190        200        210        220        230        240 
GVGDATIMEA LAVVYGGSAA MRSVVERAYN LRADLGMVAK ILATQGIDAL KNIKPEVGVP 

       250        260        270        280        290        300 
IRPMLAERLN DPVEIIKKLG GRALVEYKYD GERAQIHKKG DKIWIFSRRL ENITHMYPDV 

       310        320        330        340        350        360 
VEMAKKGIKA EEAIVEGEIV AIDPETGEFR PFQVLMHRRR KKDVHAVLSE IPVAVFLFDT 

       370        380        390        400        410        420 
LYVNGEDLTL KPLPARHEVL EKIIEQSDTW RIAEGIVTSD PQELESFFLK AIEDGAEGVM 

       430        440        450        460        470        480 
AKAIHDRSIY QAGARGWLWI KYKRDYKSEM SDTVDLVVIG AFYGKGRRGG KFGALLMAAY 

       490        500        510        520        530        540 
DPDTDTFKSV CKVGSGFTDE DLERLDDMLK PYISDKKPAR VDSQMKPDVW VEPTLVAEII 

       550        560        570        580        590        600 
GAELTLSPIH TCCYGWVKSG AGISIRFPRF IRWRPDKGPE DATTTKELYE MYKRQLRRIK 


EEEAPTGV 

« Hide

References

[1]"The genome of Hyperthermus butylicus: a sulfur-reducing, peptide fermenting, neutrophilic Crenarchaeote growing up to 108 degrees C."
Bruegger K., Chen L., Stark M., Zibat A., Redder P., Ruepp A., Awayez M., She Q., Garrett R.A., Klenk H.-P.
Archaea 2:127-135(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 5456 / JCM 9403.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000493 Genomic DNA. Translation: ABM80287.1.
RefSeqYP_001012632.1. NC_008818.1.

3D structure databases

ProteinModelPortalA2BJX6.
SMRA2BJX6. Positions 3-598.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING415426.Hbut_0421.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM80287; ABM80287; Hbut_0421.
GeneID4781994.
KEGGhbu:Hbut_0421.

Phylogenomic databases

eggNOGCOG1793.
HOGENOMHOG000036008.
KOK10747.
OMAMVKMLEG.

Enzyme and pathway databases

BioCycHBUT415426:GC56-421-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI_HYPBU
AccessionPrimary (citable) accession number: A2BJX6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: February 20, 2007
Last modified: July 9, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families