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Protein

DNA ligase

Gene

lig

Organism
Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. Can use ATP, ADP and GTP, but not CTP, TTP or NAD+.1 Publication

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.UniRule annotation1 Publication
ADP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + phosphate.1 Publication
GTP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + GMP + diphosphate.1 Publication

Cofactori

Mg2+1 Publication, Mn2+1 Publication

pH dependencei

Optimum pH is 8.0.1 Publication

Temperature dependencei

Optimum temperature is 75 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei266ATPUniRule annotation1
Active sitei268N6-AMP-lysine intermediateUniRule annotation1
Binding sitei273ATPUniRule annotation1
Binding sitei288ATPUniRule annotation1
Binding sitei318ATPUniRule annotation1
Binding sitei358ATPUniRule annotation1
Binding sitei435ATPUniRule annotation1
Binding sitei441ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication
LigandATP-binding, GTP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase1 PublicationUniRule annotation (EC:6.5.1.71 Publication)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP/ADP/GTP]Curated
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:Hbut_0421
OrganismiHyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)
Taxonomic identifieri415426 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesPyrodictiaceaeHyperthermus
Proteomesi
  • UP000002593 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003652481 – 608DNA ligaseAdd BLAST608

Proteomic databases

PRIDEiA2BJX6

Interactioni

Protein-protein interaction databases

STRINGi415426.Hbut_0421

Structurei

3D structure databases

ProteinModelPortaliA2BJX6
SMRiA2BJX6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotationCurated

Phylogenomic databases

eggNOGiarCOG01347 Archaea
COG1793 LUCA
HOGENOMiHOG000036008
KOiK10747
OMAiWIKYKRD
OrthoDBiPOG093Z03L0

Family and domain databases

Gene3Di1.10.3260.10, 1 hit
HAMAPiMF_00407 DNA_ligase, 1 hit
InterProiView protein in InterPro
IPR022865 DNA_ligae_ATP-dep_bac/arc
IPR000977 DNA_ligase_ATP-dep
IPR012309 DNA_ligase_ATP-dep_C
IPR012310 DNA_ligase_ATP-dep_cent
IPR016059 DNA_ligase_ATP-dep_CS
IPR012308 DNA_ligase_ATP-dep_N
IPR036599 DNA_ligase_N_sf
IPR012340 NA-bd_OB-fold
PfamiView protein in Pfam
PF04679 DNA_ligase_A_C, 1 hit
PF01068 DNA_ligase_A_M, 1 hit
PF04675 DNA_ligase_A_N, 1 hit
SUPFAMiSSF117018 SSF117018, 1 hit
SSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00574 dnl1, 1 hit
PROSITEiView protein in PROSITE
PS00697 DNA_LIGASE_A1, 1 hit
PS00333 DNA_LIGASE_A2, 1 hit
PS50160 DNA_LIGASE_A3, 1 hit

Sequencei

Sequence statusi: Complete.

A2BJX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMPFSVLAE TFEKLERVTA RTQMLLYLVE LFKKTPPEII DKVVYFIQGK
60 70 80 90 100
LWPDWKGLPE LGIGEKMLIK AASIALHVSE KQIEQLVKKL GDTGKAIEMI
110 120 130 140 150
KREKQQKQKA VGLLAFMQKP SGGESTLTVE KVYDTLARIA LVQGEGSRDI
160 170 180 190 200
KLKLLAGLLA EASPREAKYI ARFVEGRLRL GVGDATIMEA LAVVYGGSAA
210 220 230 240 250
MRSVVERAYN LRADLGMVAK ILATQGIDAL KNIKPEVGVP IRPMLAERLN
260 270 280 290 300
DPVEIIKKLG GRALVEYKYD GERAQIHKKG DKIWIFSRRL ENITHMYPDV
310 320 330 340 350
VEMAKKGIKA EEAIVEGEIV AIDPETGEFR PFQVLMHRRR KKDVHAVLSE
360 370 380 390 400
IPVAVFLFDT LYVNGEDLTL KPLPARHEVL EKIIEQSDTW RIAEGIVTSD
410 420 430 440 450
PQELESFFLK AIEDGAEGVM AKAIHDRSIY QAGARGWLWI KYKRDYKSEM
460 470 480 490 500
SDTVDLVVIG AFYGKGRRGG KFGALLMAAY DPDTDTFKSV CKVGSGFTDE
510 520 530 540 550
DLERLDDMLK PYISDKKPAR VDSQMKPDVW VEPTLVAEII GAELTLSPIH
560 570 580 590 600
TCCYGWVKSG AGISIRFPRF IRWRPDKGPE DATTTKELYE MYKRQLRRIK

EEEAPTGV
Length:608
Mass (Da):68,330
Last modified:February 20, 2007 - v1
Checksum:i7D16CF4AEB60771E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000493 Genomic DNA Translation: ABM80287.1
RefSeqiWP_011821605.1, NC_008818.1

Genome annotation databases

EnsemblBacteriaiABM80287; ABM80287; Hbut_0421
GeneIDi4781994
KEGGihbu:Hbut_0421

Similar proteinsi

Entry informationi

Entry nameiDNLI_HYPBU
AccessioniPrimary (citable) accession number: A2BJX6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: February 20, 2007
Last modified: May 23, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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