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A2BJL6 (ASSY_HYPBU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:Hbut_0305
OrganismHyperthermus butylicus (strain DSM 5456 / JCM 9403) [Reference proteome] [HAMAP]
Taxonomic identifier415426 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiDesulfurococcalesPyrodictiaceaeHyperthermus

Protein attributes

Sequence length396 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00005

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 396396Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_1000000399

Regions

Nucleotide binding6 – 149ATP By similarity

Sites

Binding site831Citrulline By similarity
Binding site1131ATP; via amide nitrogen By similarity
Binding site1151Aspartate By similarity
Binding site1191Aspartate By similarity
Binding site1191Citrulline By similarity
Binding site1201Aspartate By similarity
Binding site1231Citrulline By similarity
Binding site1711Citrulline By similarity
Binding site1801Citrulline By similarity
Binding site2561Citrulline By similarity
Binding site2681Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
A2BJL6 [UniParc].

Last modified February 20, 2007. Version 1.
Checksum: 6DE1CD0EAB61DDB2

FASTA39644,521
        10         20         30         40         50         60 
MKVVLAYSGG LDTSAILVLL REQGHEVVTV TVSVGQEEDL EEVEERAYKL GASKHYTVNA 

        70         80         90        100        110        120 
VEEFAEKYIS MAIKANALYE DKYPLGTALA RPLIAEKVAE IARKESADAV AHGCTSKGND 

       130        140        150        160        170        180 
QVRFDTMLKY YLGDDIKIIA PVRELRLTRA KAAEILRRNG FQPPGLHKTY SIDENLWSRS 

       190        200        210        220        230        240 
IEGGPLDDPM AEPPEDAFAW TVAPEKAPDQ PLYLEIGFEK GIPVSINGEK MHLAKIVSLL 

       250        260        270        280        290        300 
NRLVGAYGYG RIDHIENRVV GLKSREVYEA PAALTLIEAH RDLEKTVYTP RELRFKRILD 

       310        320        330        340        350        360 
QEWSDLVYQG LWVEPLRATL EKAIDELNRW VTGTVRVKVY KGSLMVVGRW SPYSGYSREA 

       370        380        390 
IDYNHGWYPS DEEARGFITI WSLHSLAAAK ARSLWG 

« Hide

References

[1]"The genome of Hyperthermus butylicus: a sulfur-reducing, peptide fermenting, neutrophilic Crenarchaeote growing up to 108 degrees C."
Bruegger K., Chen L., Stark M., Zibat A., Redder P., Ruepp A., Awayez M., She Q., Garrett R.A., Klenk H.-P.
Archaea 2:127-135(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 5456 / JCM 9403.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000493 Genomic DNA. Translation: ABM80177.1.
RefSeqYP_001012522.1. NC_008818.1.

3D structure databases

ProteinModelPortalA2BJL6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING415426.Hbut_0305.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM80177; ABM80177; Hbut_0305.
GeneID4782292.
KEGGhbu:Hbut_0305.

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230093.
KOK01940.
OMAIYNGYWW.
ProtClustDBPRK00509.

Enzyme and pathway databases

BioCycHBUT415426:GC56-305-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_HYPBU
AccessionPrimary (citable) accession number: A2BJL6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 20, 2007
Last modified: February 19, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways