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A2BIW2

- ENDA_HYPBU

UniProt

A2BIW2 - ENDA_HYPBU

Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Hyperthermus butylicus (strain DSM 5456 / JCM 9403)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (20 Feb 2007)
      Previous versions | rss
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    Functioni

    Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.UniRule annotation

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei118 – 1181UniRule annotation
    Active sitei126 – 1261UniRule annotation
    Active sitei157 – 1571UniRule annotation

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    tRNA processing

    Enzyme and pathway databases

    BioCyciHBUT415426:GC56-45-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
    Alternative name(s):
    tRNA-intron endonucleaseUniRule annotation
    Gene namesi
    Name:endAUniRule annotation
    Ordered Locus Names:Hbut_0045
    OrganismiHyperthermus butylicus (strain DSM 5456 / JCM 9403)
    Taxonomic identifieri415426 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesPyrodictiaceaeHyperthermus
    ProteomesiUP000002593: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 181181tRNA-splicing endonucleasePRO_0000309816Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers.UniRule annotation

    Protein-protein interaction databases

    STRINGi415426.Hbut_0045.

    Structurei

    3D structure databases

    ProteinModelPortaliA2BIW2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1676.
    HOGENOMiHOG000107823.
    KOiK01170.
    OMAiALKFGSH.

    Family and domain databases

    Gene3Di3.40.1350.10. 1 hit.
    HAMAPiMF_01833. EndA_short.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR016442. tRNA_splic_arch_short.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 1 hit.
    PF02778. tRNA_int_endo_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005285. tRNA_splic_archaea. 1 hit.
    SUPFAMiSSF53032. SSF53032. 1 hit.
    SSF55267. SSF55267. 1 hit.
    TIGRFAMsiTIGR00324. endA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A2BIW2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIRAVLLGLR AVVFDREAAR RLYASGYYGK PLGIPKPRGS NFDAPLELGL    50
    LETLHLLERG MLEVYDEEGH RVSVEEVRRR AERFIPKFEL LYKVYRDLRD 100
    RGYVVRSGLK YGSDFAVYER GPGLEHAPYL VHVMRVDEEI DPLEIVRAGR 150
    LSHSVRKAFI LALTGPRDTP IRYLLLKWSK P 181
    Length:181
    Mass (Da):20,892
    Last modified:February 20, 2007 - v1
    Checksum:iA1C361B1456F3696
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000493 Genomic DNA. Translation: ABM79923.1.
    RefSeqiWP_011821240.1. NC_008818.1.
    YP_001012268.1. NC_008818.1.

    Genome annotation databases

    EnsemblBacteriaiABM79923; ABM79923; Hbut_0045.
    GeneIDi4782745.
    KEGGihbu:Hbut_0045.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000493 Genomic DNA. Translation: ABM79923.1 .
    RefSeqi WP_011821240.1. NC_008818.1.
    YP_001012268.1. NC_008818.1.

    3D structure databases

    ProteinModelPortali A2BIW2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 415426.Hbut_0045.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABM79923 ; ABM79923 ; Hbut_0045 .
    GeneIDi 4782745.
    KEGGi hbu:Hbut_0045.

    Phylogenomic databases

    eggNOGi COG1676.
    HOGENOMi HOG000107823.
    KOi K01170.
    OMAi ALKFGSH.

    Enzyme and pathway databases

    BioCyci HBUT415426:GC56-45-MONOMER.

    Family and domain databases

    Gene3Di 3.40.1350.10. 1 hit.
    HAMAPi MF_01833. EndA_short.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR016442. tRNA_splic_arch_short.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 1 hit.
    PF02778. tRNA_int_endo_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005285. tRNA_splic_archaea. 1 hit.
    SUPFAMi SSF53032. SSF53032. 1 hit.
    SSF55267. SSF55267. 1 hit.
    TIGRFAMsi TIGR00324. endA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome of Hyperthermus butylicus: a sulfur-reducing, peptide fermenting, neutrophilic Crenarchaeote growing up to 108 degrees C."
      Bruegger K., Chen L., Stark M., Zibat A., Redder P., Ruepp A., Awayez M., She Q., Garrett R.A., Klenk H.-P.
      Archaea 2:127-135(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 5456 / JCM 9403.

    Entry informationi

    Entry nameiENDA_HYPBU
    AccessioniPrimary (citable) accession number: A2BIW2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2007
    Last sequence update: February 20, 2007
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3