A2BIW2 (ENDA_HYPBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-splicing endonuclease
EC=3.1.27.9

Alternative name(s):
tRNA-intron endonuclease

Gene names

Name:	endA
Ordered Locus Names:	Hbut_0045

Organism

Hyperthermus butylicus (strain DSM 5456 / JCM 9403) [Reference proteome] [HAMAP]

Taxonomic identifier

415426 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Pyrodictiaceae › Hyperthermus › Hyperthermus butylicus

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity. HAMAP-Rule MF_01833
Catalytic activity	Endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxy and 2',3'-cyclic phosphate termini, and specifically removing the intron. HAMAP-Rule MF_01833
Subunit structure	Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers By similarity.
Sequence similarities	Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA splicing, via endonucleolytic cleavage and ligation Inferred from electronic annotation. Source: HAMAP
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro tRNA-intron endonuclease activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 181	181	tRNA-splicing endonuclease HAMAP-Rule MF_01833		PRO_0000309816
Sites
	Active site	118	1	By similarity
	Active site	126	1	By similarity
	Active site	157	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2BIW2 [UniParc]. Last modified February 20, 2007. Version 1. Checksum: A1C361B1456F3696	FASTA	181	20,892
	10 20 30 40 50 60 MIRAVLLGLR AVVFDREAAR RLYASGYYGK PLGIPKPRGS NFDAPLELGL LETLHLLERG 70 80 90 100 110 120 MLEVYDEEGH RVSVEEVRRR AERFIPKFEL LYKVYRDLRD RGYVVRSGLK YGSDFAVYER 130 140 150 160 170 180 GPGLEHAPYL VHVMRVDEEI DPLEIVRAGR LSHSVRKAFI LALTGPRDTP IRYLLLKWSK P

References

[1]

"The genome of Hyperthermus butylicus: a sulfur-reducing, peptide fermenting, neutrophilic Crenarchaeote growing up to 108 degrees C." Bruegger K., Chen L., Stark M., Zibat A., Redder P., Ruepp A., Awayez M., She Q., Garrett R.A., Klenk H.-P. Archaea 2:127-135(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 5456 / JCM 9403.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000493 Genomic DNA. Translation: ABM79923.1.
RefSeq	YP_001012268.1. NC_008818.1.
3D structure databases
ProteinModelPortal	A2BIW2.
ModBase	Search...
Protein-protein interaction databases
STRING	415426.Hbut_0045.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABM79923; ABM79923; Hbut_0045.
GeneID	4782745.
KEGG	hbu:Hbut_0045.
Phylogenomic databases
eggNOG	COG1676.
HOGENOM	HOG000107823.
KO	K01170.
OMA	FVRVAHS.
ProtClustDB	PRK09297.
Enzyme and pathway databases
BioCyc	HBUT415426:GC56-45-MONOMER.
Family and domain databases
Gene3D	3.40.1350.10. 1 hit.
HAMAP	MF_01833. EndA_short.
InterPro	IPR011856. tRNA_endonuc-like_dom. IPR006677. tRNA_intron_Endonuc_cat-like. IPR006678. tRNA_intron_Endonuc_N. IPR006676. tRNA_splic. IPR016442. tRNA_splic_arch_short. [Graphical view]
Pfam	PF01974. tRNA_int_endo. 1 hit. PF02778. tRNA_int_endo_N. 1 hit. [Graphical view]
PIRSF	PIRSF005285. tRNA_splic_archaea. 1 hit.
SUPFAM	SSF53032. tRNA_int_endo_C. 1 hit. SSF55267. tRNA_int_endo_N. 1 hit.
TIGRFAMs	TIGR00324. endA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ENDA_HYPBU

Accession

Primary (citable) accession number: A2BIW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	February 20, 2007
Last modified:	May 29, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program