A2BGT0 (UBP30_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 30
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 30
Ubiquitin thioesterase 30
Ubiquitin-specific-processing protease 30
Short name=Ub-specific protease 30

Gene names

Name:	usp30
ORF Names:	si:dkey-72n1.2

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May participate in the maintenance of mitochondrial morphology By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subcellular location	Mitochondrion outer membrane By similarity.
Sequence similarities	Belongs to the peptidase C19 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Ubiquitin carboxyl-terminal hydrolase 30		PRO_0000377538
Regions
	Topological domain	1 – 31	31	Mitochondrial intermembrane Potential
	Transmembrane	32 – 52	21	Helical; Potential
	Topological domain	53 – 491	439	Cytoplasmic Potential
Sites
	Active site	73	1	Nucleophile By similarity
	Active site	432	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2BGT0 [UniParc]. Last modified February 20, 2007. Version 1. Checksum: 8F3FF87F855E4CC9	FASTA	491	55,643
	10 20 30 40 50 60 MPWCKQGTTD KLVREFLRTG AAARNKMMKN WGVIGGIAAA MAAGVYVLWG PISDRRKKRK 70 80 90 100 110 120 GMVPGLLNLG NTCFMNSLLQ GLAACPSFIR WLEDFTSQNS ADRERTERET QLSRSLMQLL 130 140 150 160 170 180 KALSSHDPGE DDVLDAGGLL EALRLYRWHI SSFEEQDAHE LFHVLTSSLE EEQERQPRVA 190 200 210 220 230 240 HLFDMQTLEK SVESKEKNIS CRSGGPLHPI PSLWRTRHPF HGRLTSYMAC KRCEQQSPVH 250 260 270 280 290 300 YDSFDSLSLS IPSIQWGRPV TLDQCLQHFI SSETIKEVEC ENCTKQQAGE LVNGEVLESQ 310 320 330 340 350 360 RTTFVKQLKL GKRLTWSKEG SPIKRQEHVQ FTEYLSLDRY KHCSAAQSQQ KTSRTNKAKA 370 380 390 400 410 420 SADPKDKAIA NGVDSEHCNN NKPQSNGTFP SVFLHSPGLS SQLNLTYDYS TSEYLFRLTA 430 440 450 460 470 480 VLVHHGDMHS GHFITYRRCP AAPRGTSPFS SQWLWVSDDS VRKASLQEVL SSSAYLLFYE 490 RMQRPGLRVE E

References

[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX511005 Genomic DNA. Translation: CAM16313.1.
IPI	IPI00498326.
RefSeq	NP_001155956.1. NM_001162484.1.
UniGene	Dr.103783.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	7955.ENSDARP00000073858.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000079402; ENSDARP00000073858; ENSDARG00000056842.
GeneID	559099.
KEGG	dre:559099.
Organism-specific databases
CTD	84749.
ZFIN	ZDB-GENE-060526-335. usp30.
Phylogenomic databases
eggNOG	NOG259866.
GeneTree	ENSGT00550000075075.
HOGENOM	HOG000065744.
InParanoid	A2BGT0.
KO	K11851.
OMA	MDIYKYR.
OrthoDB	EOG451DQH.
Gene expression databases
Bgee	A2BGT0.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20882786.

Entry information

Entry name

UBP30_DANRE

Accession

Primary (citable) accession number: A2BGT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	February 20, 2007
Last modified:	April 3, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program