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Protein

Adenylyltransferase and sulfurtransferase MOCS3

Gene

Mocs3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.UniRule annotation

Catalytic activityi

ATP + [molybdopterin-synthase sulfur-carrier protein]-Gly-Gly = diphosphate + [molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP.UniRule annotation
[Molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP + [cysteine desulfurase]-S-sulfanyl-L-cysteine + reduced acceptor = AMP + [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH(2)-C(O)SH + [cysteine desulfurase] + oxidized acceptor.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi: 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis

This protein is involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis and in tRNA modification.

Pathwayi: molybdopterin biosynthesis

This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92ATP; via amide nitrogenUniRule annotation1
Binding sitei113ATPUniRule annotation1
Binding sitei137ATPUniRule annotation1
Metal bindingi222ZincUniRule annotation1
Metal bindingi225ZincUniRule annotation1
Active sitei239Glycyl thioester intermediate; for adenylyltransferase activityUniRule annotation1
Metal bindingi297ZincUniRule annotation1
Metal bindingi300ZincUniRule annotation1
Active sitei412Cysteine persulfide intermediate; for sulfurtransferase activityUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi120 – 124ATPUniRule annotation5
Nucleotide bindingi181 – 182ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Molybdenum cofactor biosynthesis, tRNA processing

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-947581. Molybdenum cofactor biosynthesis.
UniPathwayiUPA00344.
UPA00988.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylyltransferase and sulfurtransferase MOCS3UniRule annotation
Alternative name(s):
Molybdenum cofactor synthesis protein 3UniRule annotation
Including the following 2 domains:
Molybdopterin-synthase adenylyltransferaseUniRule annotation (EC:2.7.7.80UniRule annotation)
Alternative name(s):
Adenylyltransferase MOCS3UniRule annotation
Sulfur carrier protein MOCS2A adenylyltransferaseUniRule annotation
Molybdopterin-synthase sulfurtransferaseUniRule annotation (EC:2.8.1.11UniRule annotation)
Alternative name(s):
Sulfur carrier protein MOCS2A sulfurtransferaseUniRule annotation
Sulfurtransferase MOCS3UniRule annotation
Gene namesi
Name:Mocs3
Synonyms:Uba4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1916622. Mocs3.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002819181 – 460Adenylyltransferase and sulfurtransferase MOCS3Add BLAST460

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi316 ↔ 324UniRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiA2BDX3.
MaxQBiA2BDX3.
PaxDbiA2BDX3.
PeptideAtlasiA2BDX3.
PRIDEiA2BDX3.

PTM databases

iPTMnetiA2BDX3.
PhosphoSitePlusiA2BDX3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000074576.
CleanExiMM_MOCS3.
GenevisibleiA2BDX3. MM.

Interactioni

Subunit structurei

Interacts with NFS1.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000096670.

Structurei

3D structure databases

ProteinModelPortaliA2BDX3.
SMRiA2BDX3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini347 – 458RhodaneseUniRule annotationAdd BLAST112

Sequence similaritiesi

In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily.UniRule annotation
Contains 1 rhodanese domain.UniRule annotation

Phylogenomic databases

eggNOGiKOG2017. Eukaryota.
COG0476. LUCA.
COG0607. LUCA.
GeneTreeiENSGT00810000125467.
HOGENOMiHOG000281219.
HOVERGENiHBG052491.
InParanoidiA2BDX3.
KOiK11996.
OMAiVRNPNEY.
OrthoDBiEOG091G0EQP.
TreeFamiTF106103.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_03049. MOCS3_Uba4. 1 hit.
InterProiIPR028885. MOCS3/Uba4.
IPR016040. NAD(P)-bd_dom.
IPR001763. Rhodanese-like_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
PF00899. ThiF. 1 hit.
[Graphical view]
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2BDX3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPEDVAAL QAEITRREEE LASLKRRLAA ALTAEPEPER PLRVPPPPLA
60 70 80 90 100
PRAALSRDEI LRYSRQLLLP ELGVRGQLRL AAAAVLVVGC GGLGCPLAQY
110 120 130 140 150
LAAAGVGRLG LVDHDVVETS NLARQVLHGE AQAGESKARS AAAALRRLNS
160 170 180 190 200
AVECVAYPRA LAEDWALDLV RGYDVVADCC DNVPTRYLVN DACVLAGRPL
210 220 230 240 250
VSASALRFEG QMTVYHHDGG PCYRCVFPRP PPPETVTNCA DGGVLGAVPG
260 270 280 290 300
VLGCAQALEV LKIAAGLGSS YSGSMLLFDG LGGHFRRIRL RRRRPDCVVC
310 320 330 340 350
GQQPTVTRLQ DYEAFCGSSA TDKCRALKLL CPEERISVTD YKRLLDSGAP
360 370 380 390 400
HVLLDVRPQV EVDICRLPHS LHIPLSQLER RDADSLKLLG AALRKGKQES
410 420 430 440 450
QEGVALPVYV ICKLGNDSQK AVKVLQSLTA VPELDSLTVQ DIVGGLMAWA
460
AKIDGTFPQY
Length:460
Mass (Da):49,375
Last modified:February 20, 2007 - v1
Checksum:i7340FD4B2D9826F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX005039 Genomic DNA. Translation: CAM22977.1.
CCDSiCCDS50802.1.
RefSeqiNP_001153802.1. NM_001160330.1.
UniGeneiMm.482182.

Genome annotation databases

EnsembliENSMUST00000099071; ENSMUSP00000096670; ENSMUSG00000074576.
GeneIDi69372.
KEGGimmu:69372.
UCSCiuc012cjs.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX005039 Genomic DNA. Translation: CAM22977.1.
CCDSiCCDS50802.1.
RefSeqiNP_001153802.1. NM_001160330.1.
UniGeneiMm.482182.

3D structure databases

ProteinModelPortaliA2BDX3.
SMRiA2BDX3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000096670.

PTM databases

iPTMnetiA2BDX3.
PhosphoSitePlusiA2BDX3.

Proteomic databases

EPDiA2BDX3.
MaxQBiA2BDX3.
PaxDbiA2BDX3.
PeptideAtlasiA2BDX3.
PRIDEiA2BDX3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000099071; ENSMUSP00000096670; ENSMUSG00000074576.
GeneIDi69372.
KEGGimmu:69372.
UCSCiuc012cjs.1. mouse.

Organism-specific databases

CTDi27304.
MGIiMGI:1916622. Mocs3.

Phylogenomic databases

eggNOGiKOG2017. Eukaryota.
COG0476. LUCA.
COG0607. LUCA.
GeneTreeiENSGT00810000125467.
HOGENOMiHOG000281219.
HOVERGENiHBG052491.
InParanoidiA2BDX3.
KOiK11996.
OMAiVRNPNEY.
OrthoDBiEOG091G0EQP.
TreeFamiTF106103.

Enzyme and pathway databases

UniPathwayiUPA00344.
UPA00988.
ReactomeiR-MMU-947581. Molybdenum cofactor biosynthesis.

Miscellaneous databases

PROiA2BDX3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000074576.
CleanExiMM_MOCS3.
GenevisibleiA2BDX3. MM.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_03049. MOCS3_Uba4. 1 hit.
InterProiIPR028885. MOCS3/Uba4.
IPR016040. NAD(P)-bd_dom.
IPR001763. Rhodanese-like_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
PF00899. ThiF. 1 hit.
[Graphical view]
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMOCS3_MOUSE
AccessioniPrimary (citable) accession number: A2BDX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: February 20, 2007
Last modified: November 2, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.