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A2BDX3

- MOCS3_MOUSE

UniProt

A2BDX3 - MOCS3_MOUSE

Protein

Adenylyltransferase and sulfurtransferase MOCS3

Gene

Mocs3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (20 Feb 2007)
      Previous versions | rss
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    Functioni

    Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.UniRule annotation

    Catalytic activityi

    ATP + [molybdopterin-synthase sulfur-carrier protein]-Gly-Gly = diphosphate + [molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP.UniRule annotation
    [Molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP + [cysteine desulfurase]-S-sulfanyl-L-cysteine = AMP + [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH(2)-C(O)SH + cysteine desulfurase.UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei92 – 921ATP; via amide nitrogenUniRule annotation
    Binding sitei113 – 1131ATPUniRule annotation
    Binding sitei137 – 1371ATPUniRule annotation
    Metal bindingi222 – 2221ZincUniRule annotation
    Metal bindingi225 – 2251ZincUniRule annotation
    Active sitei239 – 2391Glycyl thioester intermediate; for adenylyltransferase activityUniRule annotation
    Metal bindingi297 – 2971ZincUniRule annotation
    Metal bindingi300 – 3001ZincUniRule annotation
    Active sitei412 – 4121Cysteine persulfide intermediate; for sulfurtransferase activityUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi120 – 1245ATPUniRule annotation
    Nucleotide bindingi181 – 1822ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. molybdopterin-synthase adenylyltransferase activity Source: UniProtKB-EC
    4. molybdopterin-synthase sulfurtransferase activity Source: UniProtKB-EC
    5. nucleotidyltransferase activity Source: UniProtKB
    6. protein adenylyltransferase activity Source: RefGenome
    7. sulfurtransferase activity Source: UniProtKB
    8. thiosulfate sulfurtransferase activity Source: UniProtKB
    9. URM1 activating enzyme activity Source: UniProtKB

    GO - Biological processi

    1. enzyme active site formation via L-cysteine persulfide Source: UniProtKB
    2. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB
    3. protein adenylylation Source: GOC
    4. protein urmylation Source: RefGenome
    5. tRNA thio-modification Source: UniProtKB
    6. tRNA wobble position uridine thiolation Source: RefGenome
    7. tRNA wobble uridine modification Source: UniProtKB

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Molybdenum cofactor biosynthesis, tRNA processing

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_198541. Molybdenum cofactor biosynthesis.
    UniPathwayiUPA00344.
    UPA00988.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenylyltransferase and sulfurtransferase MOCS3UniRule annotation
    Alternative name(s):
    Molybdenum cofactor synthesis protein 3UniRule annotation
    Including the following 2 domains:
    Molybdopterin-synthase adenylyltransferaseUniRule annotation (EC:2.7.7.80UniRule annotation)
    Alternative name(s):
    Adenylyltransferase MOCS3UniRule annotation
    Sulfur carrier protein MOCS2A adenylyltransferaseUniRule annotation
    Molybdopterin-synthase sulfurtransferaseUniRule annotation (EC:2.8.1.11UniRule annotation)
    Alternative name(s):
    Sulfur carrier protein MOCS2A sulfurtransferaseUniRule annotation
    Sulfurtransferase MOCS3UniRule annotation
    Gene namesi
    Name:Mocs3
    Synonyms:Uba4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1916622. Mocs3.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytosol Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 460460Adenylyltransferase and sulfurtransferase MOCS3PRO_0000281918Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi316 ↔ 324AlternateUniRule annotation

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiA2BDX3.
    PRIDEiA2BDX3.

    PTM databases

    PhosphoSiteiA2BDX3.

    Expressioni

    Gene expression databases

    ArrayExpressiA2BDX3.
    BgeeiA2BDX3.
    CleanExiMM_MOCS3.
    GenevestigatoriA2BDX3.

    Interactioni

    Subunit structurei

    Interacts with NFS1.

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000096670.

    Structurei

    3D structure databases

    ProteinModelPortaliA2BDX3.
    SMRiA2BDX3. Positions 53-301, 335-460.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini347 – 458112RhodaneseUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily.UniRule annotation
    Contains 1 rhodanese domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0476.
    GeneTreeiENSGT00570000079161.
    HOGENOMiHOG000281219.
    HOVERGENiHBG052491.
    InParanoidiA2BDX3.
    KOiK11996.
    OMAiVRKDPEC.
    OrthoDBiEOG776SQ3.
    TreeFamiTF106103.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    3.40.50.720. 1 hit.
    HAMAPiMF_03049. MOCS3_Uba4.
    InterProiIPR028885. MOCS3/Uba4.
    IPR007901. MoeZ_MoeB.
    IPR009036. Molybdenum_cofac_synth_MoeB.
    IPR016040. NAD(P)-bd_dom.
    IPR001763. Rhodanese-like_dom.
    IPR000594. ThiF_NAD_FAD-bd.
    [Graphical view]
    PfamiPF05237. MoeZ_MoeB. 1 hit.
    PF00581. Rhodanese. 1 hit.
    PF00899. ThiF. 1 hit.
    [Graphical view]
    SMARTiSM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF69572. SSF69572. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A2BDX3-1 [UniParc]FASTAAdd to Basket

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    MAAPEDVAAL QAEITRREEE LASLKRRLAA ALTAEPEPER PLRVPPPPLA    50
    PRAALSRDEI LRYSRQLLLP ELGVRGQLRL AAAAVLVVGC GGLGCPLAQY 100
    LAAAGVGRLG LVDHDVVETS NLARQVLHGE AQAGESKARS AAAALRRLNS 150
    AVECVAYPRA LAEDWALDLV RGYDVVADCC DNVPTRYLVN DACVLAGRPL 200
    VSASALRFEG QMTVYHHDGG PCYRCVFPRP PPPETVTNCA DGGVLGAVPG 250
    VLGCAQALEV LKIAAGLGSS YSGSMLLFDG LGGHFRRIRL RRRRPDCVVC 300
    GQQPTVTRLQ DYEAFCGSSA TDKCRALKLL CPEERISVTD YKRLLDSGAP 350
    HVLLDVRPQV EVDICRLPHS LHIPLSQLER RDADSLKLLG AALRKGKQES 400
    QEGVALPVYV ICKLGNDSQK AVKVLQSLTA VPELDSLTVQ DIVGGLMAWA 450
    AKIDGTFPQY 460
    Length:460
    Mass (Da):49,375
    Last modified:February 20, 2007 - v1
    Checksum:i7340FD4B2D9826F1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX005039 Genomic DNA. Translation: CAM22977.1.
    CCDSiCCDS50802.1.
    RefSeqiNP_001153802.1. NM_001160330.1.
    UniGeneiMm.482182.

    Genome annotation databases

    EnsembliENSMUST00000099071; ENSMUSP00000096670; ENSMUSG00000074576.
    GeneIDi69372.
    KEGGimmu:69372.
    UCSCiuc012cjs.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX005039 Genomic DNA. Translation: CAM22977.1 .
    CCDSi CCDS50802.1.
    RefSeqi NP_001153802.1. NM_001160330.1.
    UniGenei Mm.482182.

    3D structure databases

    ProteinModelPortali A2BDX3.
    SMRi A2BDX3. Positions 53-301, 335-460.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000096670.

    PTM databases

    PhosphoSitei A2BDX3.

    Proteomic databases

    PaxDbi A2BDX3.
    PRIDEi A2BDX3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000099071 ; ENSMUSP00000096670 ; ENSMUSG00000074576 .
    GeneIDi 69372.
    KEGGi mmu:69372.
    UCSCi uc012cjs.1. mouse.

    Organism-specific databases

    CTDi 27304.
    MGIi MGI:1916622. Mocs3.

    Phylogenomic databases

    eggNOGi COG0476.
    GeneTreei ENSGT00570000079161.
    HOGENOMi HOG000281219.
    HOVERGENi HBG052491.
    InParanoidi A2BDX3.
    KOi K11996.
    OMAi VRKDPEC.
    OrthoDBi EOG776SQ3.
    TreeFami TF106103.

    Enzyme and pathway databases

    UniPathwayi UPA00344 .
    UPA00988 .
    Reactomei REACT_198541. Molybdenum cofactor biosynthesis.

    Miscellaneous databases

    NextBioi 329269.
    PROi A2BDX3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi A2BDX3.
    Bgeei A2BDX3.
    CleanExi MM_MOCS3.
    Genevestigatori A2BDX3.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    3.40.50.720. 1 hit.
    HAMAPi MF_03049. MOCS3_Uba4.
    InterProi IPR028885. MOCS3/Uba4.
    IPR007901. MoeZ_MoeB.
    IPR009036. Molybdenum_cofac_synth_MoeB.
    IPR016040. NAD(P)-bd_dom.
    IPR001763. Rhodanese-like_dom.
    IPR000594. ThiF_NAD_FAD-bd.
    [Graphical view ]
    Pfami PF05237. MoeZ_MoeB. 1 hit.
    PF00581. Rhodanese. 1 hit.
    PF00899. ThiF. 1 hit.
    [Graphical view ]
    SMARTi SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69572. SSF69572. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.

    Entry informationi

    Entry nameiMOCS3_MOUSE
    AccessioniPrimary (citable) accession number: A2BDX3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: February 20, 2007
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3