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Protein

Adenylyltransferase and sulfurtransferase MOCS3

Gene

Mocs3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.UniRule annotation

Catalytic activityi

ATP + [molybdopterin-synthase sulfur-carrier protein]-Gly-Gly = diphosphate + [molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP.UniRule annotation
[Molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP + [cysteine desulfurase]-S-sulfanyl-L-cysteine + reduced acceptor = AMP + [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + [cysteine desulfurase] + oxidized acceptor.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi: 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis

This protein is involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis and in tRNA modification.

Pathwayi: molybdopterin biosynthesis

This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92ATP; via amide nitrogenUniRule annotation1
Binding sitei113ATPUniRule annotation1
Binding sitei137ATPUniRule annotation1
Metal bindingi222ZincUniRule annotation1
Metal bindingi225ZincUniRule annotation1
Active sitei239Glycyl thioester intermediate; for adenylyltransferase activityUniRule annotation1
Metal bindingi297ZincUniRule annotation1
Metal bindingi300ZincUniRule annotation1
Active sitei412Cysteine persulfide intermediate; for sulfurtransferase activityUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi120 – 124ATPUniRule annotation5
Nucleotide bindingi181 – 182ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMultifunctional enzyme, Transferase
Biological processMolybdenum cofactor biosynthesis, tRNA processing
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-947581 Molybdenum cofactor biosynthesis
UniPathwayiUPA00344
UPA00988

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylyltransferase and sulfurtransferase MOCS3UniRule annotation
Alternative name(s):
Molybdenum cofactor synthesis protein 3UniRule annotation
Including the following 2 domains:
Molybdopterin-synthase adenylyltransferaseUniRule annotation (EC:2.7.7.80UniRule annotation)
Alternative name(s):
Adenylyltransferase MOCS3UniRule annotation
Sulfur carrier protein MOCS2A adenylyltransferaseUniRule annotation
Molybdopterin-synthase sulfurtransferaseUniRule annotation (EC:2.8.1.11UniRule annotation)
Alternative name(s):
Sulfur carrier protein MOCS2A sulfurtransferaseUniRule annotation
Sulfurtransferase MOCS3UniRule annotation
Gene namesi
Name:Mocs3
Synonyms:Uba4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1916622 Mocs3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002819181 – 460Adenylyltransferase and sulfurtransferase MOCS3Add BLAST460

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi316 ↔ 324UniRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiA2BDX3
MaxQBiA2BDX3
PaxDbiA2BDX3
PeptideAtlasiA2BDX3
PRIDEiA2BDX3

PTM databases

iPTMnetiA2BDX3
PhosphoSitePlusiA2BDX3

Expressioni

Gene expression databases

BgeeiENSMUSG00000074576
CleanExiMM_MOCS3
GenevisibleiA2BDX3 MM

Interactioni

Subunit structurei

Interacts with NFS1.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000096670

Structurei

3D structure databases

ProteinModelPortaliA2BDX3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini347 – 458RhodaneseUniRule annotationAdd BLAST112

Sequence similaritiesi

In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2017 Eukaryota
COG0476 LUCA
COG0607 LUCA
GeneTreeiENSGT00810000125467
HOGENOMiHOG000281219
HOVERGENiHBG052491
InParanoidiA2BDX3
KOiK11996
OMAiMAWAAKI
OrthoDBiEOG091G0EQP
TreeFamiTF106103

Family and domain databases

Gene3Di3.40.250.10, 1 hit
HAMAPiMF_03049 MOCS3_Uba4, 1 hit
InterProiView protein in InterPro
IPR028885 MOCS3/Uba4
IPR001763 Rhodanese-like_dom
IPR036873 Rhodanese-like_dom_sf
IPR000594 ThiF_NAD_FAD-bd
IPR035985 Ubiquitin-activating_enz
PfamiView protein in Pfam
PF00581 Rhodanese, 1 hit
PF00899 ThiF, 1 hit
SMARTiView protein in SMART
SM00450 RHOD, 1 hit
SUPFAMiSSF69572 SSF69572, 1 hit
PROSITEiView protein in PROSITE
PS50206 RHODANESE_3, 1 hit

Sequencei

Sequence statusi: Complete.

A2BDX3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPEDVAAL QAEITRREEE LASLKRRLAA ALTAEPEPER PLRVPPPPLA
60 70 80 90 100
PRAALSRDEI LRYSRQLLLP ELGVRGQLRL AAAAVLVVGC GGLGCPLAQY
110 120 130 140 150
LAAAGVGRLG LVDHDVVETS NLARQVLHGE AQAGESKARS AAAALRRLNS
160 170 180 190 200
AVECVAYPRA LAEDWALDLV RGYDVVADCC DNVPTRYLVN DACVLAGRPL
210 220 230 240 250
VSASALRFEG QMTVYHHDGG PCYRCVFPRP PPPETVTNCA DGGVLGAVPG
260 270 280 290 300
VLGCAQALEV LKIAAGLGSS YSGSMLLFDG LGGHFRRIRL RRRRPDCVVC
310 320 330 340 350
GQQPTVTRLQ DYEAFCGSSA TDKCRALKLL CPEERISVTD YKRLLDSGAP
360 370 380 390 400
HVLLDVRPQV EVDICRLPHS LHIPLSQLER RDADSLKLLG AALRKGKQES
410 420 430 440 450
QEGVALPVYV ICKLGNDSQK AVKVLQSLTA VPELDSLTVQ DIVGGLMAWA
460
AKIDGTFPQY
Length:460
Mass (Da):49,375
Last modified:February 20, 2007 - v1
Checksum:i7340FD4B2D9826F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX005039 Genomic DNA No translation available.
CCDSiCCDS50802.1
RefSeqiNP_001153802.1, NM_001160330.1
UniGeneiMm.482182

Genome annotation databases

EnsembliENSMUST00000099071; ENSMUSP00000096670; ENSMUSG00000074576
GeneIDi69372
KEGGimmu:69372
UCSCiuc012cjs.1 mouse

Similar proteinsi

Entry informationi

Entry nameiMOCS3_MOUSE
AccessioniPrimary (citable) accession number: A2BDX3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: February 20, 2007
Last modified: April 25, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health