Reviewed, UniProtKB/Swiss-Prot A2AV36 (ANM7_DANRE)

Names and origin

Protein names

Recommended name:
    Protein arginine N-methyltransferase 7
    EC=2.1.1.-
Alternative name(s):
    Histone-arginine N-methyltransferase PRMT7
    EC=2.1.1.125
    [Myelin basic protein]-arginine N-methyltransferase PRMT7
    EC=2.1.1.126

Gene names

Name:	prmt7
ORF Names:	ch211-51l3.2

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	683 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3sme2. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3sme2, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo By similarity.
Catalytic activity	S-adenosyl-L-methionine + histone-arginine = S-adenosyl-L-homocysteine + histone-N(omega)-methyl-arginine. S-adenosyl-L-methionine + [myelin basic protein]-arginine = S-adenosyl-L-homocysteine + [myelin basic protein]-N(omega)-methyl-arginine.
Subcellular location	Cytoplasm › cytosol By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the protein arginine N-methyltransferase family. PRMT7 subfamily.
Sequence caution	The sequence AAH44492.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence.

Ontologies

Keywords
Biological process	Differentiation Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation during gametogenesis Inferred from sequence or structural similarity. Source: UniProtKB cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW genetic imprinting Inferred from sequence or structural similarity. Source: UniProtKB histone arginine methylation Inferred from sequence or structural similarity. Source: UniProtKB peptidyl-arginine methylation Inferred from sequence or structural similarity. Source: UniProtKB regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW spliceosomal snRNP assembly Inferred from sequence or structural similarity. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	[myelin basic protein]-arginine N-methyltransferase activity Inferred from electronic annotation. Source: EC histone methyltransferase activity (H4-R3 specific) Inferred from sequence or structural similarity. Source: UniProtKB protein-arginine omega-N symmetric methyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 683	683	Protein arginine N-methyltransferase 7		PRO_0000373904
Experimental info
	Sequence conflict	59	1	V → I in AAH44492. Ref.2
	Sequence conflict	348	1	E → K in AAH44492. Ref.2
	Sequence conflict	393	1	I → M in AAH44492. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	A2AV36-1 [UniParc]. Last modified February 20, 2007. Version 1. Checksum: 33AB7ED83B972589	FASTA	683	76,547
	10 20 30 40 50 60 MKTFCGRANP TTGALDWVEE SEEYDYHQEI ARSCYADMLH DKDRNEKYYE GIRAAVRRVK 70 80 90 100 110 120 ARGERPVVLD IGTGTGLLSM MAVTAGADFC YAIEVFKPMA QAASCIVERN GFSDKIKIIN 130 140 150 160 170 180 KHSTEVTVGP DGDMQERANI LVTELFDTEL IGEGALPSYE HAHMHLVQTG CEAVPHRATI 190 200 210 220 230 240 YAQLVESDML WKWAQMRPID VDGHRLMPPG AVQECAGAPS VCDIQLSQVP TDAFTAISPV 250 260 270 280 290 300 CTMFSVDFSK PVSSAAQSYT VRFKSQTGGR AQVVLSWWDI DMDPEGNIVC TMAPSWSYAD 310 320 330 340 350 360 PHAYPWRDHW MQSVYFLPAE ENVSEGEELM LMVSHDDYSL WYSLTHSEQN DVRVAPFRPC 370 380 390 400 410 420 CTCQAHLVWT RPRFGELNDE QRTESYVSAL RSILKPDSVC LSVSDGSLLP VFAHLLGSKK 430 440 450 460 470 480 VFSLESSGMA KQVIEQVLHT NSLKDGVQLL GIRAEQLSLA DLDGNQISVL MGEPYFSTSL 490 500 510 520 530 540 LPWHSLFFWY CRTAVAQLLQ PDATILPRAA TLYAVAVEFQ DLWRIRFPCG TCEGFDVSPM 550 560 570 580 590 600 DEMIQRSLDF RESWEAEPHP LWEYPCRALT KPCPVMTFDF TQCVPEQPIS SDGAVPFTGR 610 620 630 640 650 660 GRCHGVALWM EYQLTDDISV SMGLTKAVSQ EGACEWNPHR KQGVFFFRSA KETSGDGRED 670 680 LSYSLTFEPH SGDIKMDFSI TES

References

[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-682. Strain: AB.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL929309 Genomic DNA. Translation: CAM14259.1. BC044492 mRNA. Translation: AAH44492.1. Sequence problems.
IPI	IPI00482900.
RefSeq	NP_956797.2.
UniGene	Dr.3855
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSDART00000073609; ENSDARP00000068099; ENSDARG00000051902; Danio rerio. [Genome view]
GeneID	393475.
KEGG	dre:393475.
Organism-specific databases
CTD	393475.
ZFIN	ZDB-GENE-040426-1560. prmt7.
Phylogenomic databases
HOVERGEN	A2AV36.
Gene expression databases
Bgee	A2AV36.
Family and domain databases
InterPro	IPR014644. Arg_N-MeTrfase. [Graphical view]
PIRSF	PIRSF036946. Arg_N-mtase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ANM7_DANRE

Accession

Primary (citable) accession number: A2AV36
Secondary accession number(s): Q803G6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	February 20, 2007
Last modified:	October 13, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project