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Protein

Methyltransferase-like protein 8

Gene

Mettl8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable methyltransferase (By similarity). Isoform 5 overexpression in lung progenitor cells stimulates smooth muscle-specific gene expression and suppresses adipogenic gene expression. Isoform 4 and isoform 7 stimulate adipogenesis.By similarity2 Publications

GO - Molecular functioni

  • histone acetyltransferase activity Source: MGI
  • methyltransferase activity Source: UniProtKB-KW

GO - Biological processi

  • fat cell differentiation Source: MGI
  • histone acetylation Source: MGI
  • skeletal muscle tissue development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Methyltransferase-like protein 8 (EC:2.1.1.-)
Alternative name(s):
Tension-induced/inhibited protein
Gene namesi
Name:Mettl8
Synonyms:Tip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2385142. Mettl8.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

  • Note: Isoform 5 localizes to the nucleus upon cell stretch in embryonic lung progenitor cells.

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 281281Methyltransferase-like protein 8PRO_0000311292Add
BLAST

Proteomic databases

MaxQBiA2AUU0.
PaxDbiA2AUU0.
PRIDEiA2AUU0.

PTM databases

PhosphoSiteiA2AUU0.

Expressioni

Tissue specificityi

Isoform 5 is absent in undifferentiated embryonic lung mesenchymal cells, but expression is induced by stretch. Isoform 4 is expressed in undifferentiated progenitor cells, while its expression is inhibited by stretch. Isoform 2 is absent in embryonic lung but is induced in a fibroblast cell line by stretch. Isoform 7 is expressed in mature adipose tissue.1 Publication

Inductioni

Isoform 5 is induced in stretched embryonic lung mesenchymal cells.1 Publication

Gene expression databases

BgeeiA2AUU0.
CleanExiMM_METTL8.
ExpressionAtlasiA2AUU0. baseline and differential.
GenevisibleiA2AUU0. MM.

Interactioni

Subunit structurei

Interacts with EP300.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000107804.

Structurei

3D structure databases

ProteinModelPortaliA2AUU0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2361. Eukaryota.
ENOG410YAN4. LUCA.
GeneTreeiENSGT00520000055554.
HOVERGENiHBG075344.
InParanoidiA2AUU0.
OMAiLSENFYT.
PhylomeDBiA2AUU0.
TreeFamiTF323232.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR026113. MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR22809. PTHR22809. 2 hits.
PIRSFiPIRSF037755. Mettl2_prd. 1 hit.
SUPFAMiSSF53335. SSF53335. 2 hits.

Sequences (9)i

Sequence statusi: Complete.

This entry describes 9 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A2AUU0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNVIWRSCIC RLRQGKVPHR CQSGVHPVAP LGSRILTDPA KVFEHNMWDH
60 70 80 90 100
MQWSKEEEDA ARKKVEENSA TRVAPEEQVK FESDANKYWD IFYQTHKNKF
110 120 130 140 150
FKNRNWLLRE FPEILPVNQN TKEKVGESSW DQVGSSISRT QGTETHCQES
160 170 180 190 200
FVSPEPGSRG RSAPDPDLEE YSKGPGKTEP FPGSNATFRI LEVGCGAGNS
210 220 230 240 250
VFPILNTLQN IPGSFLYCCD FASEAVELVK SHESYSEAQC SAFIHDVCDD
260 270 280
GLAYPFPDGI LDVVLLVFVL SSIHPDRALF I
Note: Gene prediction based on similarity to human ortholog.
Length:281
Mass (Da):31,670
Last modified:November 13, 2007 - v2
Checksum:i2B6DD620DA56FBFB
GO
Isoform 2 (identifier: A2AUU0-2) [UniParc]FASTAAdd to basket

Also known as: TIP-2

The sequence of this isoform differs from the canonical sequence as follows:
     278-281: ALFI → QVPPCLPNRTCDFYKMSQPPGRGYRPA

Show »
Length:304
Mass (Da):34,287
Checksum:i3E305C84AF58A734
GO
Isoform 3 (identifier: A2AUU0-3) [UniParc]FASTAAdd to basket

Also known as: TIP-4

The sequence of this isoform differs from the canonical sequence as follows:
     278-281: ALFI → MQAVAHRLSR...PSPWTPQSGN

Show »
Length:388
Mass (Da):44,337
Checksum:i330B0C33D6CC0D5A
GO
Isoform 4 (identifier: A2AUU0-4) [UniParc]FASTAAdd to basket

Also known as: TIP-3

The sequence of this isoform differs from the canonical sequence as follows:
     278-281: ALFI → MQAVAHRLSR...SKIWLIIACK

Show »
Length:358
Mass (Da):40,433
Checksum:i0F6F24A7787AAB23
GO
Isoform 5 (identifier: A2AUU0-5) [UniParc]FASTAAdd to basket

Also known as: TIP-1

The sequence of this isoform differs from the canonical sequence as follows:
     193-281: VGCGAGNSVF...SIHPDRALFI → EHSRILSLLLRLCL

Show »
Length:206
Mass (Da):23,753
Checksum:i3DC0134B9FF30A17
GO
Isoform 6 (identifier: A2AUU0-6) [UniParc]FASTAAdd to basket

Also known as: TIP-5

The sequence of this isoform differs from the canonical sequence as follows:
     79-154: Missing.
     278-281: ALFI → MQAVAHRLSR...PSPWTPQSGN

Show »
Length:312
Mass (Da):35,341
Checksum:i5364FE73A0F38FB1
GO
Isoform 7 (identifier: A2AUU0-7)

Also known as: TIP-6

Sequence is not available
Length:
Mass (Da):
Isoform 8 (identifier: A2AUU0-8)

Also known as: TIP-7

Sequence is not available
Length:
Mass (Da):
Isoform 9 (identifier: A2AUU0-9)

Also known as: TIP-8

Sequence is not available
Length:
Mass (Da):

Sequence cautioni

The sequence AAH04636.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH57960.1 differs from that shown.Sequence differs from the displayed sequence in the N-terminal region.Curated
The sequence AAZ04166.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAZ04167.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti91 – 911I → T in ABB54392 (PubMed:18710950).Curated
Sequence conflicti91 – 911I → T in AAH04636 (PubMed:15489334).Curated
Sequence conflicti91 – 911I → T in AAH57960 (PubMed:15489334).Curated
Sequence conflicti161 – 1611R → W in AAZ04166 (PubMed:15992539).Curated
Sequence conflicti161 – 1611R → W in AAZ04167 (PubMed:15992539).Curated
Sequence conflicti161 – 1611R → W in ABB54392 (PubMed:18710950).Curated
Sequence conflicti161 – 1611R → W in ABB54393 (PubMed:18710950).Curated
Sequence conflicti161 – 1611R → W in AAH04636 (PubMed:15489334).Curated
Sequence conflicti161 – 1611R → W in AAH57960 (PubMed:15489334).Curated
Sequence conflicti233 – 2331E → K in AAZ04167 (PubMed:15992539).Curated
Sequence conflicti233 – 2331E → K in ABB54392 (PubMed:18710950).Curated
Sequence conflicti233 – 2331E → K in ABB54393 (PubMed:18710950).Curated
Sequence conflicti233 – 2331E → K in AAH04636 (PubMed:15489334).Curated
Sequence conflicti233 – 2331E → K in AAH57960 (PubMed:15489334).Curated
Sequence conflicti238 – 2381A → T in AAZ04167 (PubMed:15992539).Curated
Sequence conflicti238 – 2381A → T in ABB54392 (PubMed:18710950).Curated
Sequence conflicti238 – 2381A → T in ABB54393 (PubMed:18710950).Curated
Sequence conflicti238 – 2381A → T in AAH04636 (PubMed:15489334).Curated
Sequence conflicti238 – 2381A → T in AAH57960 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei79 – 15476Missing in isoform 6. 1 PublicationVSP_037111Add
BLAST
Alternative sequencei193 – 28189VGCGA…RALFI → EHSRILSLLLRLCL in isoform 5. 1 PublicationVSP_029515Add
BLAST
Alternative sequencei278 – 2814ALFI → QVPPCLPNRTCDFYKMSQPP GRGYRPA in isoform 2. 2 PublicationsVSP_029516
Alternative sequencei278 – 2814ALFI → MQAVAHRLSRLLKPGGMLLF RDHGRYDNAQLRFKKGRCLS ENFYVRGDGTRAYFFTKGEI RRMFCEAGLHEKQNLVDHRL QVNRKKQVQMHRVWIQGKFQ KPSPWTPQSGN in isoform 3 and isoform 6. 2 PublicationsVSP_029517
Alternative sequencei278 – 2814ALFI → MQAVAHRLSRLLKPGGMLLF RDHGRYDNAQLRFKKGRCLS ENFYVRGDGPELISLQKGKS AVCSARLDYTKSKIWLIIAC K in isoform 4. 1 PublicationVSP_029518

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ093357 mRNA. Translation: AAZ04166.1. Different initiation.
DQ093358 mRNA. Translation: AAZ04167.1. Different initiation.
DQ230973 mRNA. Translation: ABB54392.1.
DQ230974 mRNA. Translation: ABB54393.1.
AL929228 Genomic DNA. Translation: CAM22148.2.
AL929228 Genomic DNA. Translation: CAM22151.2.
BC004636 mRNA. Translation: AAH04636.1. Different initiation.
BC057960 mRNA. Translation: AAH57960.1. Sequence problems.
CCDSiCCDS50600.1. [A2AUU0-3]
RefSeqiNP_001103982.1. NM_001110512.1. [A2AUU0-6]
NP_663499.2. NM_145524.3. [A2AUU0-3]
XP_006499258.1. XM_006499195.2. [A2AUU0-6]
UniGeneiMm.490343.

Genome annotation databases

EnsembliENSMUST00000112179; ENSMUSP00000107800; ENSMUSG00000041975. [A2AUU0-5]
ENSMUST00000112186; ENSMUSP00000107804; ENSMUSG00000041975. [A2AUU0-3]
ENSMUST00000121586; ENSMUSP00000113642; ENSMUSG00000041975. [A2AUU0-1]
ENSMUST00000148876; ENSMUSP00000115855; ENSMUSG00000041975. [A2AUU0-2]
GeneIDi228019.
KEGGimmu:228019.
UCSCiuc008jzw.3. mouse. [A2AUU0-6]
uc008jzx.3. mouse. [A2AUU0-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ093357 mRNA. Translation: AAZ04166.1. Different initiation.
DQ093358 mRNA. Translation: AAZ04167.1. Different initiation.
DQ230973 mRNA. Translation: ABB54392.1.
DQ230974 mRNA. Translation: ABB54393.1.
AL929228 Genomic DNA. Translation: CAM22148.2.
AL929228 Genomic DNA. Translation: CAM22151.2.
BC004636 mRNA. Translation: AAH04636.1. Different initiation.
BC057960 mRNA. Translation: AAH57960.1. Sequence problems.
CCDSiCCDS50600.1. [A2AUU0-3]
RefSeqiNP_001103982.1. NM_001110512.1. [A2AUU0-6]
NP_663499.2. NM_145524.3. [A2AUU0-3]
XP_006499258.1. XM_006499195.2. [A2AUU0-6]
UniGeneiMm.490343.

3D structure databases

ProteinModelPortaliA2AUU0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000107804.

PTM databases

PhosphoSiteiA2AUU0.

Proteomic databases

MaxQBiA2AUU0.
PaxDbiA2AUU0.
PRIDEiA2AUU0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000112179; ENSMUSP00000107800; ENSMUSG00000041975. [A2AUU0-5]
ENSMUST00000112186; ENSMUSP00000107804; ENSMUSG00000041975. [A2AUU0-3]
ENSMUST00000121586; ENSMUSP00000113642; ENSMUSG00000041975. [A2AUU0-1]
ENSMUST00000148876; ENSMUSP00000115855; ENSMUSG00000041975. [A2AUU0-2]
GeneIDi228019.
KEGGimmu:228019.
UCSCiuc008jzw.3. mouse. [A2AUU0-6]
uc008jzx.3. mouse. [A2AUU0-3]

Organism-specific databases

CTDi79828.
MGIiMGI:2385142. Mettl8.

Phylogenomic databases

eggNOGiKOG2361. Eukaryota.
ENOG410YAN4. LUCA.
GeneTreeiENSGT00520000055554.
HOVERGENiHBG075344.
InParanoidiA2AUU0.
OMAiLSENFYT.
PhylomeDBiA2AUU0.
TreeFamiTF323232.

Miscellaneous databases

NextBioi378897.
PROiA2AUU0.
SOURCEiSearch...

Gene expression databases

BgeeiA2AUU0.
CleanExiMM_METTL8.
ExpressionAtlasiA2AUU0. baseline and differential.
GenevisibleiA2AUU0. MM.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR026113. MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR22809. PTHR22809. 2 hits.
PIRSFiPIRSF037755. Mettl2_prd. 1 hit.
SUPFAMiSSF53335. SSF53335. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "TIPs are tension-responsive proteins involved in myogenic versus adipogenic differentiation."
    Jakkaraju S., Zhe X., Pan D., Choudhury R., Schuger L.
    Dev. Cell 9:39-49(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 4 AND 5), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
    Strain: CD-1.
  2. "Effects of the SANT domain of tension-induced/inhibited proteins (TIPs), novel partners of the histone acetyltransferase p300, on p300 activity and TIP-6-induced adipogenesis."
    Badri K.R., Zhou Y., Dhru U., Aramgam S., Schuger L.
    Mol. Cell. Biol. 28:6358-6372(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 6), FUNCTION, INTERACTION WITH EP300, ALTERNATIVE SPLICING (ISOFORMS 7; 8 AND 9).
    Strain: NIH Swiss.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 49-281 (ISOFORM 3).
    Strain: FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiMETL8_MOUSE
AccessioniPrimary (citable) accession number: A2AUU0
Secondary accession number(s): A2AUU3
, Q2XV35, Q2XV36, Q4FCR9, Q4FCS0, Q6PEN9, Q99KI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 13, 2007
Last modified: November 11, 2015
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

According to PubMed:15992539 and PubMed:18710950, contains a SANT domain but this domain is not detected by any prediction tool.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.