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Protein

Integrin alpha-8

Gene

Itga8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Integrin alpha-8/beta-1 functions in the genesis of kidney and probably of other organs by regulating the recruitment of mesenchymal cells into epithelial structures. It recognizes the sequence R-G-D in a wide array of ligands including TNC, FN1, SPP1 TGFB1, TGFB3 and VTN. NPNT is probably its functional ligand in kidney genesis. Neuronal receptor for TNC it mediates cell-cell interactions and regulates neurite outgrowth of sensory and motor neurons.8 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi328 – 336Sequence analysis9
Calcium bindingi394 – 402Sequence analysis9
Calcium bindingi458 – 466Sequence analysis9

GO - Molecular functioni

GO - Biological processi

  • brain development Source: Ensembl
  • cell-matrix adhesion Source: MGI
  • cell projection organization Source: MGI
  • establishment of protein localization Source: BHF-UCL
  • extracellular matrix organization Source: MGI
  • inner ear morphogenesis Source: MGI
  • integrin-mediated signaling pathway Source: UniProtKB-KW
  • kidney development Source: MGI
  • memory Source: MGI
  • mesodermal cell differentiation Source: Ensembl
  • metanephros development Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter involved in smooth muscle cell differentiation Source: BHF-UCL
  • positive regulation of transforming growth factor beta receptor signaling pathway Source: MGI
  • smooth muscle tissue development Source: BHF-UCL
  • substrate adhesion-dependent cell spreading Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Integrin, Receptor

Keywords - Biological processi

Cell adhesion, Differentiation, Neurogenesis

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2129379. Molecules associated with elastic fibres.
R-MMU-216083. Integrin cell surface interactions.
R-MMU-3000178. ECM proteoglycans.

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin alpha-8
Cleaved into the following 2 chains:
Gene namesi
Name:Itga8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:109442. Itga8.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini36 – 1010ExtracellularSequence analysisAdd BLAST975
Transmembranei1011 – 1031HelicalSequence analysisAdd BLAST21
Topological domaini1032 – 1062CytoplasmicSequence analysisAdd BLAST31

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice display renal agenesis and dysgenesis. This is associated with a reduced expression of Gdnf that is similarly found in mice lacking Npnt. Adult mice also display increased susceptibility to glomerular capillary destruction upon mechanical stress. Mice lacking Itga8 also have difficulty balancing associated with structural defects in the inner ear where utricular hair cells lack stereocilia.4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 35Sequence analysisAdd BLAST35
ChainiPRO_000029770936 – 1062Integrin alpha-8Add BLAST1027
ChainiPRO_000029771038 – 905Integrin alpha-8 heavy chainSequence analysisAdd BLAST868
ChainiPRO_0000297711906 – 1062Integrin alpha-8 light chainSequence analysisAdd BLAST157

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi80N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi95 ↔ 105By similarity
Glycosylationi121N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi149 ↔ 170By similarity
Glycosylationi176N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi186 ↔ 199By similarity
Glycosylationi238N-linked (GlcNAc...)Sequence analysis1
Glycosylationi301N-linked (GlcNAc...)Sequence analysis1
Glycosylationi310N-linked (GlcNAc...)Sequence analysis1
Glycosylationi503N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi506 ↔ 517By similarity
Disulfide bondi523 ↔ 579By similarity
Glycosylationi600N-linked (GlcNAc...)Sequence analysis1
Glycosylationi604N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi640 ↔ 646By similarity
Disulfide bondi712 ↔ 725By similarity
Glycosylationi718N-linked (GlcNAc...)Sequence analysis1
Glycosylationi736N-linked (GlcNAc...)Sequence analysis1
Glycosylationi752N-linked (GlcNAc...)Sequence analysis1
Glycosylationi779N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi866 ↔ 923Interchain (between heavy and light chains)By similarity
Glycosylationi895N-linked (GlcNAc...)Sequence analysis1
Glycosylationi922N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi928 ↔ 933By similarity
Glycosylationi1004N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiA2ARA8.
PaxDbiA2ARA8.
PeptideAtlasiA2ARA8.
PRIDEiA2ARA8.

PTM databases

iPTMnetiA2ARA8.
PhosphoSitePlusiA2ARA8.

Expressioni

Tissue specificityi

In brain, expressed in deep cortex, hippocampal CA1, basolateral amygdala and striatum. In kidney, expressed in glomerular mesengium (at protein level).2 Publications

Developmental stagei

Expressed in mesenchymal cells of developing organs such as gut, lung, gonads and nephrogenic cord.2 Publications

Gene expression databases

BgeeiENSMUSG00000026768.
CleanExiMM_ITGA8.
ExpressionAtlasiA2ARA8. baseline and differential.
GenevisibleiA2ARA8. MM.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-8 associates with beta-1.

Protein-protein interaction databases

IntActiA2ARA8. 1 interactor.
MINTiMINT-4105300.
STRINGi10090.ENSMUSP00000028106.

Structurei

3D structure databases

ProteinModelPortaliA2ARA8.
SMRiA2ARA8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati41 – 104FG-GAP 1PROSITE-ProRule annotationAdd BLAST64
Repeati121 – 182FG-GAP 2PROSITE-ProRule annotationAdd BLAST62
Repeati187 – 239FG-GAP 3PROSITE-ProRule annotationAdd BLAST53
Repeati252 – 305FG-GAP 4PROSITE-ProRule annotationAdd BLAST54
Repeati306 – 371FG-GAP 5PROSITE-ProRule annotationAdd BLAST66
Repeati372 – 430FG-GAP 6PROSITE-ProRule annotationAdd BLAST59
Repeati434 – 497FG-GAP 7PROSITE-ProRule annotationAdd BLAST64

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi454 – 456Cell attachment siteSequence analysis3

Sequence similaritiesi

Belongs to the integrin alpha chain family.Curated
Contains 7 FG-GAP repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3637. Eukaryota.
ENOG410XPVZ. LUCA.
GeneTreeiENSGT00760000118782.
HOGENOMiHOG000231603.
HOVERGENiHBG006186.
InParanoidiA2ARA8.
KOiK06584.
OMAiMCIPDLK.
OrthoDBiEOG091G05Z4.
PhylomeDBiA2ARA8.
TreeFamiTF105391.

Family and domain databases

InterProiIPR013517. FG-GAP.
IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
IPR032695. Integrin_dom.
[Graphical view]
PfamiPF01839. FG-GAP. 2 hits.
PF00357. Integrin_alpha. 1 hit.
PF08441. Integrin_alpha2. 1 hit.
[Graphical view]
PRINTSiPR01185. INTEGRINA.
SMARTiSM00191. Int_alpha. 6 hits.
[Graphical view]
SUPFAMiSSF69179. SSF69179. 3 hits.
PROSITEiPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A2ARA8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAGTHCGPP GNRAPPFARL CCVSAALGML WSPACLAFNL DVDKLTVYSG
60 70 80 90 100
PEGSYFGYSL DFYIPDARTA SVLVGAPKAN TSQPDIVEGG AVYYCPWPSE
110 120 130 140 150
RSAQCKQIPF DTTNNRKIRV NGTKEPIEFK SNQWFGATVR AHKGKVVACA
160 170 180 190 200
PLYHWRTLKP NPAKDPVGTC YVAIQNFSAY AEHSPCRNSN ADPEGQGYCQ
210 220 230 240 250
AGFSLDFYKN GDLIVGGPGS FYWQGQVITV SIADIIANYS FKDILRKLAA
260 270 280 290 300
EKQTDVAPAS YDDSYLGYSV AAGEFTGDSQ QELVAGIPRG AQNFGYVSII
310 320 330 340 350
NSTDMTFIQN FTGEQMASYF GYTVVVSDVN NDGMDDILVG APLFMEREFE
360 370 380 390 400
SNPREVGQVY LYLQASALLF QDPQVLTGTE TFGRFGSSVA HLGDLNQDGY
410 420 430 440 450
NDIAIGVPFA GKDQRGKVLI YNGNPRGLHS KPSQVLQGIW GSQTIPSGFG
460 470 480 490 500
FSLRGDADID KNDYPDLLVG AFGKGKVAVY RARPVVTVDA QLLLHPMIIN
510 520 530 540 550
LENKTCQIPE FPTPVACFSV RVCASIAGQS ISNTIALLAE VQLDFLKQKG
560 570 580 590 600
AIKRTLFLHN HQSHFTFPFV MKQQKSLHCQ DFMVYLRDET EFRDKLSPIN
610 620 630 640 650
ISLNYSLDDS TFKDSLEVKP ILNHYRDNVV TEQAHILVDC GEDNLCVPDL
660 670 680 690 700
KLSARPDKHQ IIIGDENHLM LIINARNEGE GAYEAELFVI IPEEADYVGI
710 720 730 740 750
ERNNKGLRPL SCEYKMENVT RMVVCDLGNP MVTGTNFSLG LRFAVPRLEK
760 770 780 790 800
TNMSINFDLQ IRSSNKDNPD SNFERVQINI TAIAQVEIRG VSHPPQIVLP
810 820 830 840 850
IHNWEPEKKP HKEEEVGPLV EHIYELHNIG PSTISDSILD VGWPFSARDE
860 870 880 890 900
FLLYIFHLQT LGPLQCQTNP EINPQDIKPA ASPEDTPELS AFLRNATIPH
910 920 930 940 950
LVRKRDVPVV QLHRQSPARI LNCTNIDCLQ ISCAVGRLGG GESAVLKVRS
960 970 980 990 1000
RLWAHTFLKR KNDHYALASL VSFEVKKMPY KEQPAKLPAG STAVKTSVIW
1010 1020 1030 1040 1050
ATPNVSFSIP LWVIILAILL GLLVLAILTL ALWKCGFFDR ARPPQDEMTD
1060
REQLTSDKTP EA
Length:1,062
Mass (Da):117,556
Last modified:February 20, 2007 - v1
Checksum:iDB1ACA43FADD3AC0
GO
Isoform 2 (identifier: A2ARA8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     282-286: ELVAG → GQRHP
     287-1062: Missing.

Note: No experimental confirmation available.
Show »
Length:286
Mass (Da):31,011
Checksum:iDB7BCE40C46F2921
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti66D → G in BAE22455 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_027364282 – 286ELVAG → GQRHP in isoform 2. 1 Publication5
Alternative sequenceiVSP_027365287 – 1062Missing in isoform 2. 1 PublicationAdd BLAST776

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031326 mRNA. Translation: BAC27348.1.
AK044910 mRNA. Translation: BAC32137.1.
AK135193 mRNA. Translation: BAE22455.1.
AL845313 Genomic DNA. Translation: CAM17354.1.
AF041409 mRNA. Translation: AAC15665.1.
CCDSiCCDS15689.1. [A2ARA8-1]
RefSeqiNP_001001309.1. NM_001001309.3. [A2ARA8-1]
UniGeneiMm.329997.

Genome annotation databases

EnsembliENSMUST00000028106; ENSMUSP00000028106; ENSMUSG00000026768. [A2ARA8-1]
GeneIDi241226.
KEGGimmu:241226.
UCSCiuc008ijk.1. mouse. [A2ARA8-1]
uc008ijl.1. mouse. [A2ARA8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031326 mRNA. Translation: BAC27348.1.
AK044910 mRNA. Translation: BAC32137.1.
AK135193 mRNA. Translation: BAE22455.1.
AL845313 Genomic DNA. Translation: CAM17354.1.
AF041409 mRNA. Translation: AAC15665.1.
CCDSiCCDS15689.1. [A2ARA8-1]
RefSeqiNP_001001309.1. NM_001001309.3. [A2ARA8-1]
UniGeneiMm.329997.

3D structure databases

ProteinModelPortaliA2ARA8.
SMRiA2ARA8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiA2ARA8. 1 interactor.
MINTiMINT-4105300.
STRINGi10090.ENSMUSP00000028106.

PTM databases

iPTMnetiA2ARA8.
PhosphoSitePlusiA2ARA8.

Proteomic databases

MaxQBiA2ARA8.
PaxDbiA2ARA8.
PeptideAtlasiA2ARA8.
PRIDEiA2ARA8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028106; ENSMUSP00000028106; ENSMUSG00000026768. [A2ARA8-1]
GeneIDi241226.
KEGGimmu:241226.
UCSCiuc008ijk.1. mouse. [A2ARA8-1]
uc008ijl.1. mouse. [A2ARA8-2]

Organism-specific databases

CTDi8516.
MGIiMGI:109442. Itga8.

Phylogenomic databases

eggNOGiKOG3637. Eukaryota.
ENOG410XPVZ. LUCA.
GeneTreeiENSGT00760000118782.
HOGENOMiHOG000231603.
HOVERGENiHBG006186.
InParanoidiA2ARA8.
KOiK06584.
OMAiMCIPDLK.
OrthoDBiEOG091G05Z4.
PhylomeDBiA2ARA8.
TreeFamiTF105391.

Enzyme and pathway databases

ReactomeiR-MMU-2129379. Molecules associated with elastic fibres.
R-MMU-216083. Integrin cell surface interactions.
R-MMU-3000178. ECM proteoglycans.

Miscellaneous databases

PROiA2ARA8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026768.
CleanExiMM_ITGA8.
ExpressionAtlasiA2ARA8. baseline and differential.
GenevisibleiA2ARA8. MM.

Family and domain databases

InterProiIPR013517. FG-GAP.
IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
IPR032695. Integrin_dom.
[Graphical view]
PfamiPF01839. FG-GAP. 2 hits.
PF00357. Integrin_alpha. 1 hit.
PF08441. Integrin_alpha2. 1 hit.
[Graphical view]
PRINTSiPR01185. INTEGRINA.
SMARTiSM00191. Int_alpha. 6 hits.
[Graphical view]
SUPFAMiSSF69179. SSF69179. 3 hits.
PROSITEiPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiITA8_MOUSE
AccessioniPrimary (citable) accession number: A2ARA8
Secondary accession number(s): O70304
, Q3UXV8, Q8BRG3, Q8C0H7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: February 20, 2007
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.