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Protein

Ras-specific guanine nucleotide-releasing factor RalGPS1

Gene

Ralgps1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor for the small GTPase RALA. May be involved in cytoskeleton organization.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-specific guanine nucleotide-releasing factor RalGPS1
Alternative name(s):
Ral GEF with PH domain and SH3-binding motif 1
Ral guanine nucleotide exchange factor 2
Short name:
RalGEF 2
RalA exchange factor RalGPS1
Gene namesi
Name:Ralgps1
Synonyms:Kiaa0351, Ralgef2, Ralgps1a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1922008. Ralgps1.

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity

  • Note: Associates with membranes through the PH domain.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 585585Ras-specific guanine nucleotide-releasing factor RalGPS1PRO_0000333193Add
BLAST

Proteomic databases

EPDiA2AR50.
MaxQBiA2AR50.
PaxDbiA2AR50.
PRIDEiA2AR50.

PTM databases

PhosphoSiteiA2AR50.

Expressioni

Gene expression databases

BgeeiA2AR50.
ExpressionAtlasiA2AR50. baseline and differential.
GenevisibleiA2AR50. MM.

Interactioni

Subunit structurei

Interacts with the SH3 domains of GRB2, NCK1, PLCG1 and SRC.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000088563.

Structurei

Secondary structure

1
585
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi462 – 47312Combined sources
Beta strandi484 – 4918Combined sources
Beta strandi494 – 50310Combined sources
Helixi508 – 5103Combined sources
Beta strandi516 – 5205Combined sources
Beta strandi525 – 5273Combined sources
Beta strandi537 – 5415Combined sources
Beta strandi547 – 5526Combined sources
Helixi556 – 57015Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DTCX-ray1.70A/B460-585[»]
ProteinModelPortaliA2AR50.
SMRiA2AR50. Positions 44-288, 460-575.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiA2AR50.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini50 – 289240Ras-GEFPROSITE-ProRule annotationAdd
BLAST
Domaini459 – 571113PHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni461 – 585125Required for stimulation of nucleotide exchange by RALABy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi330 – 3334PXXP

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi21 – 266Poly-Ser

Domaini

The PH domain mediates binding to membranes. It is required for efficient GEF activity.By similarity

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Ras-GEF domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IQ6R. Eukaryota.
ENOG410YV9P. LUCA.
GeneTreeiENSGT00810000125349.
HOVERGENiHBG059258.
InParanoidiA2AR50.
OMAiPNWRVPI.
OrthoDBiEOG70KGP7.
PhylomeDBiA2AR50.
TreeFamiTF352150.

Family and domain databases

Gene3Di1.10.840.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00617. RasGEF. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00147. RasGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A2AR50-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYKRNGLMAS VLVTSATPQG SSSSDSLEGQ SCDYASKSYD AVVFDVLKVT
60 70 80 90 100
PEEFASQITL MDIPVFKAIQ PEELASCGWS KKEKHSLAPN VVAFTRRFNQ
110 120 130 140 150
VSFWVVREIL TAQTLKIRAE ILSHFVKIAK KLLELNNLHS LMSVVSALQS
160 170 180 190 200
APIFRLTKTW ALLNRKDKTT FEKLDYLMSK EDNYKRTRDY IRSLKMVPSI
210 220 230 240 250
PYLGIYLLDL IYIDSAYPAS GSIMENEQRS NQMNNILRII ADLQVSCSYD
260 270 280 290 300
HLTTLPHVQK YLKSVRYIEE LQKFVEDDNY KLSLRIEPGS SSPRLVSSKE
310 320 330 340 350
DLAGPSAGSS SARFRRRPTC PDTSVAGSLP TPPVPRHRKS HSLGNNLMCQ
360 370 380 390 400
LSVVESKSAT FPSEKARHLL DDSVLESRSP RRGLTHTSST AITNGLSLGS
410 420 430 440 450
SESSEFSEEM SAGLESRGRL YATLGPNWRV PVRNSPRTRS CVYSPTSPCT
460 470 480 490 500
CTVGSSATVP TMEGPLRRKT LLKEGRKPAL SSWTRYWVVL SGATLLYYGA
510 520 530 540 550
KSLRGTDRKH YKSTPGKKVS IVGWMVQLPD DPEHPDIFQL NNPDKGNVYK
560 570 580
FQTGSRFHAI LWHKHLDDAC KSSRPQVPAN LMSFE
Length:585
Mass (Da):65,475
Last modified:May 20, 2008 - v2
Checksum:i96579853188EC18F
GO
Isoform 2 (identifier: A2AR50-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     56-72: Missing.
     346-417: NLMCQLSVVESKSATFPSEKARHLLDDSVLESRSPRRGLTHTSSTAITNGLSLGSSESSEFSEEMSAGLESR → K

Note: No experimental confirmation available.
Show »
Length:497
Mass (Da):55,978
Checksum:i40706000BB66742C
GO
Isoform 3 (identifier: A2AR50-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     347-416: Missing.

Show »
Length:515
Mass (Da):58,033
Checksum:i883D07A31390E943
GO
Isoform 4 (identifier: A2AR50-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     205-243: IYLLDLIYID...NNILRIIADL → MSLSYFIINF...MFPGSSRDHG
     244-585: Missing.

Note: No experimental confirmation available.
Show »
Length:243
Mass (Da):27,500
Checksum:iBAC2A471BA44EE23
GO

Sequence cautioni

The sequence BAC25535.1 differs from that shown. Reason: Frameshift at positions 84 and 123. Curated
The sequence BAC25535.1 differs from that shown. Reason: Erroneous termination at position 158. Translated as Lys.Curated
The sequence BAC97933.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAM22215.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAM25545.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti96 – 961R → L in BAC25535 (PubMed:16141072).Curated
Sequence conflicti100 – 1001Q → H in BAC25535 (PubMed:16141072).Curated
Sequence conflicti108 – 1081E → K in BAC25535 (PubMed:16141072).Curated
Sequence conflicti118 – 1181R → K in BAC25535 (PubMed:16141072).Curated
Sequence conflicti123 – 13412SHFVK…KKLLE → TPCPVKNRPNKLSNN in BAC25535 (PubMed:16141072).CuratedAdd
BLAST
Sequence conflicti138 – 1381L → H in BAC25535 (PubMed:16141072).Curated
Sequence conflicti150 – 1556SAPIFR → IATIFL in BAC25535 (PubMed:16141072).Curated
Sequence conflicti162 – 1621L → P in AAH72656 (PubMed:15489334).Curated
Sequence conflicti165 – 1651R → L in BAC25535 (PubMed:16141072).Curated
Sequence conflicti168 – 1681K → M in BAC25535 (PubMed:16141072).Curated
Sequence conflicti174 – 1741L → P in BAC25535 (PubMed:16141072).Curated
Sequence conflicti183 – 1831N → I in BAC25535 (PubMed:16141072).Curated
Sequence conflicti204 – 2041G → C in BAC25535 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei56 – 7217Missing in isoform 2. 1 PublicationVSP_033482Add
BLAST
Alternative sequencei205 – 24339IYLLD…IIADL → MSLSYFIINFPGNITCSGPN KMFLQFLVEMFPGSSRDHG in isoform 4. 1 PublicationVSP_033483Add
BLAST
Alternative sequencei244 – 585342Missing in isoform 4. 1 PublicationVSP_033484Add
BLAST
Alternative sequencei346 – 41772NLMCQ…GLESR → K in isoform 2. 1 PublicationVSP_033485Add
BLAST
Alternative sequencei347 – 41670Missing in isoform 3. 1 PublicationVSP_033486Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129123 mRNA. Translation: BAC97933.1. Different initiation.
AK017939 mRNA. Translation: BAC25535.1. Sequence problems.
AK076767 mRNA. Translation: BAC36473.1.
AL845277, AL929197 Genomic DNA. Translation: CAM22214.1.
AL845277, AL929197 Genomic DNA. Translation: CAM22215.1. Sequence problems.
AL845277, AL929197 Genomic DNA. Translation: CAM22216.1.
AL845277 Genomic DNA. Translation: CAM22217.1.
AL929197, AL845277 Genomic DNA. Translation: CAM25544.1.
AL929197, AL845277 Genomic DNA. Translation: CAM25545.1. Sequence problems.
AL929197, AL845277 Genomic DNA. Translation: CAM25546.1.
BC072656 mRNA. Translation: AAH72656.1.
CCDSiCCDS15939.1. [A2AR50-1]
CCDS71031.1. [A2AR50-2]
CCDS79779.1. [A2AR50-3]
RefSeqiNP_001277499.1. NM_001290570.1. [A2AR50-2]
NP_001277501.1. NM_001290572.1. [A2AR50-3]
NP_780420.1. NM_175211.5. [A2AR50-1]
XP_006498095.1. XM_006498032.2. [A2AR50-1]
UniGeneiMm.274249.

Genome annotation databases

EnsembliENSMUST00000042615; ENSMUSP00000048451; ENSMUSG00000038831. [A2AR50-2]
ENSMUST00000091039; ENSMUSP00000088563; ENSMUSG00000038831. [A2AR50-1]
ENSMUST00000131298; ENSMUSP00000118363; ENSMUSG00000038831. [A2AR50-3]
GeneIDi241308.
KEGGimmu:241308.
UCSCiuc008jhm.2. mouse. [A2AR50-1]
uc008jhn.2. mouse. [A2AR50-3]
uc008jho.2. mouse. [A2AR50-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129123 mRNA. Translation: BAC97933.1. Different initiation.
AK017939 mRNA. Translation: BAC25535.1. Sequence problems.
AK076767 mRNA. Translation: BAC36473.1.
AL845277, AL929197 Genomic DNA. Translation: CAM22214.1.
AL845277, AL929197 Genomic DNA. Translation: CAM22215.1. Sequence problems.
AL845277, AL929197 Genomic DNA. Translation: CAM22216.1.
AL845277 Genomic DNA. Translation: CAM22217.1.
AL929197, AL845277 Genomic DNA. Translation: CAM25544.1.
AL929197, AL845277 Genomic DNA. Translation: CAM25545.1. Sequence problems.
AL929197, AL845277 Genomic DNA. Translation: CAM25546.1.
BC072656 mRNA. Translation: AAH72656.1.
CCDSiCCDS15939.1. [A2AR50-1]
CCDS71031.1. [A2AR50-2]
CCDS79779.1. [A2AR50-3]
RefSeqiNP_001277499.1. NM_001290570.1. [A2AR50-2]
NP_001277501.1. NM_001290572.1. [A2AR50-3]
NP_780420.1. NM_175211.5. [A2AR50-1]
XP_006498095.1. XM_006498032.2. [A2AR50-1]
UniGeneiMm.274249.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DTCX-ray1.70A/B460-585[»]
ProteinModelPortaliA2AR50.
SMRiA2AR50. Positions 44-288, 460-575.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000088563.

PTM databases

PhosphoSiteiA2AR50.

Proteomic databases

EPDiA2AR50.
MaxQBiA2AR50.
PaxDbiA2AR50.
PRIDEiA2AR50.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042615; ENSMUSP00000048451; ENSMUSG00000038831. [A2AR50-2]
ENSMUST00000091039; ENSMUSP00000088563; ENSMUSG00000038831. [A2AR50-1]
ENSMUST00000131298; ENSMUSP00000118363; ENSMUSG00000038831. [A2AR50-3]
GeneIDi241308.
KEGGimmu:241308.
UCSCiuc008jhm.2. mouse. [A2AR50-1]
uc008jhn.2. mouse. [A2AR50-3]
uc008jho.2. mouse. [A2AR50-2]

Organism-specific databases

CTDi9649.
MGIiMGI:1922008. Ralgps1.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IQ6R. Eukaryota.
ENOG410YV9P. LUCA.
GeneTreeiENSGT00810000125349.
HOVERGENiHBG059258.
InParanoidiA2AR50.
OMAiPNWRVPI.
OrthoDBiEOG70KGP7.
PhylomeDBiA2AR50.
TreeFamiTF352150.

Miscellaneous databases

ChiTaRSiRalgps1. mouse.
EvolutionaryTraceiA2AR50.
NextBioi384973.
PROiA2AR50.
SOURCEiSearch...

Gene expression databases

BgeeiA2AR50.
ExpressionAtlasiA2AR50. baseline and differential.
GenevisibleiA2AR50. MM.

Family and domain databases

Gene3Di1.10.840.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00617. RasGEF. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00147. RasGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
    Strain: C57BL/6J.
    Tissue: Testis and Thymus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Brain.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiRGPS1_MOUSE
AccessioniPrimary (citable) accession number: A2AR50
Secondary accession number(s): A2AR51
, A2AR52, Q6GQS4, Q6ZQD2, Q8BVR9, Q8C1I2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: March 16, 2016
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.