A2AQW0 (M3K15_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitogen-activated protein kinase kinase kinase 15
EC=2.7.11.25

Alternative name(s):
MAPK/ERK kinase kinase 15
Short name=MEK kinase 15
Short name=MEKK 15

Gene names

Name:

Map3k15

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1331 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May function in a signal transduction pathway that is activated by various cell stresses and leads to apoptosis By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. UniProtKB Q99683
Cofactor	Magnesium By similarity. UniProtKB Q99683
Enzyme regulation	Contains an N-terminal autoinhibitory domain. Activated by phosphorylation at Thr-816, inhibited by phosphorylation at Ser-928 By similarity. UniProtKB Q99683
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. UniProtKB Q99683 Contains 1 protein kinase domain.

Ontologies

Keywords
Domain	Coiled coil
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	MAPK cascade Inferred from electronic annotation. Source: GOC activation of MAPKK activity Inferred from electronic annotation. Source: GOC
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1331	1331	Mitogen-activated protein kinase kinase kinase 15		PRO_0000307633
Regions
	Domain	656 – 912	257	Protein kinase
	Nucleotide binding	662 – 670	9	ATP By similarity UniProtKB P28523
	Coiled coil	1216 – 1236	21	Potential
	Compositional bias	3 – 86	84	Gly-rich
	Compositional bias	949 – 952	4	Poly-Ser
	Compositional bias	1254 – 1257	4	Poly-Pro
Sites
	Active site	777	1	Proton acceptor By similarity UniProtKB P28523
	Binding site	685	1	ATP By similarity UniProtKB Q99683

Sequences

	Sequence		Length	Mass (Da)
	A2AQW0 [UniParc]. Last modified February 20, 2007. Version 1. Checksum: 6BB38F2B8A59B942	FASTA	1,331	149,327
	10 20 30 40 50 60 MEGGGGSGGG GGPVPAEAPE EAGEPPQGRL PPGPEGAAGL AEPESTGDAA GGEAEGGRGP 70 80 90 100 110 120 RRALRAVYVR SESSQGAAAG GGPEAGALKC LLRACEAEGA HLTSVPFGEL DFGETAVLDA 130 140 150 160 170 180 FYDADVAIVD MSDISRQPSL FYHLGVRESF DMANNVILYY DTDADTALSL KDMVTQKNTA 190 200 210 220 230 240 SSGNYYFIPY TVTPCADYFC CESDAQRRAS EYMQPNWDTI LGPLCMPLVD RFTSLLKDIR 250 260 270 280 290 300 VTSCAYYKET LLNDIRKARE KYQGDELAKE LTRIKFRMDN IEVLTSDIII NLLLSYRDIQ 310 320 330 340 350 360 DYDAMVKLVE TLKMLPTCDL ADQHNIKFHY AFALNRRNST GDREKALQVM LQVLQSCDHP 370 380 390 400 410 420 APDMFCLCGR IYKDIFLDSG CEEDASRDSA IEWYRKGFEL QSSLYSGINL AVLLIVSGQQ 430 440 450 460 470 480 FETSMELRKI GVRLNSLLGR KGSLEKMNNY WDVGQFFTVS MLASDIGKAV QAAERLFKLK 490 500 510 520 530 540 PPVWYLRSLV QNLLLIQRFK KPITEHSPRQ ERLNFWLDII FEATNEVTNG LRFPVLVIEP 550 560 570 580 590 600 TKVYQPSYVS INNEAEERTV SLWHVSPTEM KQIHEWNFTA SSIKGISLSK FDERCCFLYV 610 620 630 640 650 660 HDNSDDFQIY FSTEDQCNRF CSLVKEMLNN GVGSTVELEG EADGDTLEYE YDHDANGERV 670 680 690 700 710 720 VLGKGSYGIV YAGRDLSNQV RIAIKEIPER DIRYSQPLHE EIALHKYLKH RNIVQYLGSV 730 740 750 760 770 780 SENGYIKIFM EQVPGGSLSA LLRSKWGPMK EPTIKFYTKQ ILEGLKYLHE NQIVHRDIKG 790 800 810 820 830 840 DNVLVNTYSG VVKISDFGTS KRLAGINPCT ETFTGTLQYM APEIIDQGPR GYGAPADIWS 850 860 870 880 890 900 LGCTIIEMAT SRPPFHELGE PQAAMFKVGM FKIHPEIPEA LSAEARAFIL SCFEPDPQKR 910 920 930 940 950 960 VTAADLLQEG FLRQVNKGKK NRIAFKPSEG VRSGTGTLAL PSSGELVGSS SSEHGSISPD 970 980 990 1000 1010 1020 SDAQPDAFFE KVQVPKHQLS HLLSVPDESP ALDDRSTALP PEERDPGLFL LRKDSERRAI 1030 1040 1050 1060 1070 1080 LYRILWEEQN QVASNLQECV VQSSEELLLS VSHIKQIIGI LRDFIRSPEH RVMAATISKL 1090 1100 1110 1120 1130 1140 KVDLDFDSSS INQIHLILFG FQDAVNRILR NHLIRPHWMF AMDNIIRRAV QAAVTILIPE 1150 1160 1170 1180 1190 1200 LQAHFEPASE TEGVDKDTEV EGDYPLVDLL SQEVHVTPRG TRPGSVAIQE GQPHQQDPSL 1210 1220 1230 1240 1250 1260 QLSKLRQETN RLWEHLVQKE REYQNLLRLI LDQKTQELYH LQLQYKSNGG TENPPPPDGL 1270 1280 1290 1300 1310 1320 GTDRELIDWL QLQGVDANTI EKIVEEDYTL SDILNDITKE DLRCLRLRGG VLCRLWHAVS 1330 QHRRQMQESS Q

References

[1]

"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL845167, AL929452 Genomic DNA. Translation: CAM22391.1. AL929452, AL845167 Genomic DNA. Translation: CAM21674.1.
IPI	IPI00465657.
RefSeq	NP_001156557.2. NM_001163085.2.
UniGene	Mm.386889.
3D structure databases
ProteinModelPortal	A2AQW0.
SMR	A2AQW0. Positions 616-973.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000033665.
PTM databases
PhosphoSite	A2AQW0.
Proteomic databases
PaxDb	A2AQW0.
PRIDE	A2AQW0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000033665; ENSMUSP00000033665; ENSMUSG00000031303.
GeneID	270672.
KEGG	mmu:270672.
UCSC	uc009utb.2. mouse.
Organism-specific databases
CTD	389840.
MGI	MGI:2448588. Map3k15.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00700000104073.
HOGENOM	HOG000293286.
HOVERGEN	HBG006305.
InParanoid	A2AQW0.
KO	K13986.
OrthoDB	EOG4XPQF3.
Gene expression databases
Bgee	A2AQW0.
Genevestigator	A2AQW0.
Family and domain databases
InterPro	IPR025136. DUF4071. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR013761. SAM/pointed. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF13281. DUF4071. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF47769. SAM_homology. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	MAP3K15. mouse.
NextBio	393369.
SOURCE	Search...

Entry information

Entry name

M3K15_MOUSE

Accession

Primary (citable) accession number: A2AQW0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	February 20, 2007
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program